Austrian Journal of Technical and
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Scopus, Web of ScienceAustriya-11-12,2019 (2) - копия
Materials and Methods:
Organohalogen compounds. This class includes organochlorines such as chlorendic acidderivatives chlorinated paraffins; organobro mines such as decabromodiphenyl ether (decaBDE), decabromodiphenyl ethane (a replacement for decaBDE), polymeric brominated compounds such as brominated polystyrenes, brominated carbonate oligomers (BCOs), brominated epoxy oligomers (BEOs),tetrabromophthalic tetrabromobisphenol nyhydride, (TBBPA) cyclododecaneand hexabromo (HBCD). Most but not all halogenated flame-retardants are used in conjunction with a synergist to enhance their efficiency. Antimony trioxide is widely used but other forms of antimony such as the pentoxide and sodium antimonate are also used. However, those substances are contained in fodder in the form of complex indigestible conglomerates bound by complex organic compounds — phytates and cannot be digested by birds. One of the solutions of this a variety of physical and chemical factors, such as the composition of the growth medium, critical issue is the introduction into the poultry diet of phytase. 4 phytase availability of phosphorus, calcium, protein and other nutrients is limited. Critical issue is the introduction into the poultry diet of phytase. Phytase is a specific enzyme that initiates the decomposition of phytate — phytic compounds, which contain 70– 90% of the total phosphorus in plant. Due to the inability of poultry to produce own phytase availability of phosphorus, calcium, protein and other nutrients is limited. The use of phytase provides a new qualitative approach to improving fodder efficiency and reducing nutrients consumption per unit of livestock production. In spite of the fact that phytases from several species of bacteria, yeast and fungi have been characterized, commercial production of this enzyme currently focuses on fungi of the Aspergillus because of high productivity [1, 585; 2, 581]. However, great drawback of industrial fungal strains is their low activity, despite the fact that the essential requirement to enzymes is their high activity. The production of phytase have been affected by a variety of physical and chemical factors, such as the composition of the growth medium. Due to the inability of poultry to produce own Effect of Carbon and Nitrogen Sources: Different pure carbon sources (e. g. glucose, sucrose, lactose, maltose, fructose and xylose) with concentration of 1,5% were added to up the fact that the essential.Among the various nitrogen sources such as ammonium sulfate, ammonium phosphate, ammonium nitrate, potassium nitrate, yeast extract, peptone used for phytase production, ammonium nitrate supported phytase production in A. niger with enzymatic activity of 1320 U/g. All other nitrogen sources used had less effect on phytase production with enzymatic activity from 850 U/ml to 1200 U/g. The various concentration of ammonium nitrate (0.25% — 2.0%) have been used for the phytase production in submerged fermentation (Fig. 4). It was found that 0.5% ammonium nitrate has given the highest enzymatic activity (1320 U/g). Thus, among the different nitrogen and carbon sources tested 0.5% ammonium nitrate and 1.0% sucrose were found to be good nitrogen and carbon sources for phytase production. The effect of different concentration of sucrose on phytase production was studied using sucrose in the range of 0.25 to 2% (Fig.2). Sucrose at 1.0% concentration was found to support maximal phytase activity (1250 U/g). Download 1.6 Mb. Do'stlaringiz bilan baham: |
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