Best scientific research 2022

BEST SCIENTIFIC
RESEARCH - 2022 
305 
AN ALTERNATIVE OPTION TO MANUFACTURE BABY TEST 
Kurbanova Mehribonu Bahadir kizi 
Mustafakulov Muhammadjon Abduvalievich 
Hamroyeva Firangiz Nemat kizi 
Jizzakh branch of the National University of Uzbekistan 
Abstract: One of the most commonly used medical diagnostic tests is the pregnancy 
test. Although pregnancy tests look very simple, they are actually used a lot. Everything from 
the outer plastic shell to the immunoassay strip to the paper strip and the antibodies on it 
are mechanically or chemically designed based on a known principle. Determination of 
human chorionic gonadotropin (HGT) in biological fluids (urine) is of great interest for early 
diagnosis of pregnancy, assessment of pregnancy disorders, as a tumor marker, as a 
screening procedure for anti-doping control and many other purposes. For the measurement 
of HGT in urine samples, a simple sandwich-type UltraMicro enzyme is prepared and 
developed based on the coupled immunosorbent assay. Strips coated with MAB 
(monoclonal antibody) directed against HGT are used as the solid phase to ensure the 
specificity of the assay. 
Keywords: Antibody, chorionic gonadotropin, embryo, immunoSorbent, enzyme, 
placental cells. 
Pregnancy tests work by detecting the presence of the hormone human chorionic 
gonadotropin (HGT). HGT is a hormone produced by the cells of the placenta. Its production 
begins from the moment the developing embryo attaches to the uterus, 6-12 days after 
conception [1]. It works on the basis that latex particles coated with anti-HGT antibodies 
react with female urine. If HGT is present in the urine, an agglutination reaction is observed. 
Certain blood or serum beta tests can be used to measure HGT levels down to 1 mIU/ml, 
while urine test strips detect limits between 10 mIU/ml and 100 mIU/ml. Qualitative blood 

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