Chassis Parts jukebox : Meta-device : few strains


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Chassis

  • Parts jukebox : Meta-device : few strains

  • Focus of system designs & integration

  • Standardization, composability, abstraction

  • Interface with environment, power supply

  • Interface with computers: CAD, measurements

  • Human practice: Safety & process engineering


Chassis Goals

  • Genome Engineering - Multiplex Automation

  • Accelerated evolution (GE-MASS targeting)

  • Decelerated evolution in production

  • (e.g. Tn- & antimutators)

  • Data > model > CAD for efficient allele replacement

  • Why genome-wide chassis engineering?

  • Codon changes for multi-virus resistance

  • Eliminate transposons (Tn-)

  • Change restriction sites

  • Orthogonal expression systems & compartments



Synergy with Thrusts & Testbeds

  • Parts & Devices – chassis for surface display devices, eliminate restriction sites (CTAG); Construction Optimization Group (COG) meetings; IGEM

  • Human Practices – motivation for genome isolation (via codons), ecological-testing, http://www.ia-sb.eu/

  • Tumor-killing bacterium (TKB) – inherent release hence safety features – metabolic “disabling tech”

  • Microbial Chemical Factory (MCF) – multi-promoter & multi-RBS tuning via GE-MASS; Tn- stability







Why Minigenomes





Despite early success in 1968, assembly of ribosomes under conditions compatible with protein synthesis has been elusive





Improving process yield & safety: trade-off or win-win?

  • What do most biological systems fear?



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