Crispr-cas9 and beyond: what’s next in plant genome engineering

Document Outline

• CRISPR-Cas9 and beyond: what’s next in plant genome engineering
  • Abstract
  • Room for improvement: generations of genome engineering tools
    • Meganucleases
    • Zinc finger nucleases
    • TAL effector nucleases
    • CRISPR-Cas nucleases
  • Nothing was the same: the CRISPR-Cas revolution
    • SpCas9 variants
    • SpCas9 orthologs
    • Cas12a (Cpf1)
    • Cms1
    • Cas13a
    • Base editors
    • Prime editors
  • Views: applications of genome editing in plants
    • Homology-directed repair
    • Multiplex editing
    • Protein-coding edits
    • Non-coding edits
    • Off-target edits
  • Conclusions
  • References