Dual Contrast Molecular Imaging Allows Noninvasive Characterization of Myocardial Ischemia/Reperfusion Injury After Coronary Vessel Occlusion in Mice by mri running title
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§ § * * * * * § 1.2 1.15 1.1 1.05 1 0.95 0.9 0.85 0.8 CA-injection Gd-injec c ti ti on o o WT T WT c on on t WT WT WT L L LIB IB IB S S * § § § § § § * * * * * * * * * * * § § 5 1.155 1 1.11 5 1.055 111 5 0 0. 0 5 95 9 5 5 9 0. 0. 9 9 9 5 5 0 0. 85 85 8 5 5 8 8 0. 0. 0 8 8 8 8 by guest on December 22, 2017 http://circ.ahajournals.org/ Downloaded from LIBS-MPIO control-
MPIO pre LIBS-MPIO 15‘ post LIBS-MPIO 22‘ post LIBS-MPIO 37‘ post LIBS-MPIO 30‘ post Gd pre control-MPIO 30‘ post Gd 37‘ post control-MPIO 22‘ post control-MPIO 15‘ post control-MPIO Figure 5
A B re LIBS-MPIO 15‘ post LIBS-MPIO 22‘ post LIBS-MPIO 37‘ post LIBS-MPIO 30‘ post by guest on December 22, 2017 http://circ.ahajournals.org/ Downloaded from
Figure 6 A CA-injection Gd-injection P2Y12 control-MPIO P2Y12 LIBS-MPIO 1.2 1.15 1.1 1.05 1 0.95 0.9 0.85 0.8 TTC in WT TTC in P2Y 12 -/- B
n=5 LGE-Area in % of left ve ntri cl e 0 5 10 15 20 25 p=0.0026 C D 30 40 50 60 70 35 40 45 50 55 % a rea at risk % planim etri c sy stoli c re duction Infarct size in histology Ejection fraction p=0.0087 p=0.0887 P2 P2 P2 Y1 Y1 Y1 2 2 2 co co c nt n ro P2 P2 P2 Y1 Y1 Y1 2 2 2 LI LI LIBS BS BS -M -M - 0.999 0.8555 0.888 LGE -Area in LGE Area in LGE Area in 0 5 10 C C C D D D 70 70 70 55 55 55 d d uc uc ti t n n o I I f t t i i hi t t t l g g g y y y I I f t t i i i hi t t t l In In I fa fa rc rc rc t t t si si s ze ze i i i n n n hi t st st ol ol o og og g g g Ej Ej i ti f t i i Ej Ej i ti f t i i Ej j Ej ec ec e ti ti on on on f a ra ra ct ct c o io io n n n p= p= 0 0 0. 00 00 00 87 87 87 p= p p 0. 0 08 8 87 87 by guest on December 22, 2017 http://circ.ahajournals.org/ Downloaded from CD41-IHC of ischemic areas; x20 CD41/Gr1-IHC of ischemic areas; x20 A B
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n=6 n=13 P2Y 12 -/- p=0.0001 R 2 =0,8976 P2Y 12 -/- p=0.0001 R 2 =0,8976 100 m
100 m Figure 7
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100 0 20 80 60 40 p<0.01 Pl atel ets in i schemi c are a p=0.0004 140 120 100 80 60 40 20 0 p=0.0016 Pl atel et-n eutrophil-conjugate s 5 10 0 15 20 25 30 p=0.0001 R 2 =0.8976 CD CD CD41 41 4 -I C HC HC o o f f f is h ch chem em ic areas; x20 CD41/G r1 r1 -I - HC H H of ischemic are re re as; 2 x2 x20 0 0 E E F F E E E E 100
0 m m 100 m 12 1 1 0 10 10 0 0 0 80 80 60 0 0 01 0 0 01 p< p< 0. 0. 0 01 0 0 P la t el el el et e s n in i i i s s 60 60 40 40 4 20 0 = = p= 0. 0 00 p op op hi hi h l- co c c nj j ug ug ug at at at es es es 15 1 20 20 5 25 25 0 0 30 by guest on December 22, 2017 http://circ.ahajournals.org/ Downloaded from P2Y 12 -/- p<0.0001 R 2 =0.6551 p=0.0289 R 2 =0.7360 A B C D E Figure 8 p=0.0087 R 2 =0.8516 Necrosi s in % of left v entri cl e p=0.0009 5 10 15 20 25 30 Necrosi s in % of left v entri cl e n.s. 30 25 20 15 10 5 0 D D D E E E p= p p= 0. 0. 0 0 R 2 =0 Necrosi s in 5 10 0 5 0 by guest on December 22, 2017 http://circ.ahajournals.org/ Downloaded from Heidt, Peter Bronsert, Christoph Bode, Karlheinz Peter and Constantin von zur Muhlen Wang, Maximilian Mauler, Irene Neudorfer, Marius Menza, Marco Idzko, Andreas Zirlik, Timo Dominik von Elverfeldt, Alexander Maier, Daniel Duerschmied, Moritz Braig, Thilo Witsch, Xiaowei
Print ISSN: 0009-7322. Online ISSN: 1524-4539 Copyright © 2014 American Heart Association, Inc. All rights reserved. is published by the American Heart Association, 7272 Greenville Avenue, Dallas, TX 75231 Circulation published online June 20, 2014; Circulation.
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MOVIE LEGEND Movie 1 In vitro flow chamber adhesion assay showing minimal binding of control-MPIOs to microthrombi. In vitro flow chamber adhesion assays were performed using collagen-coated glass capillaries. Microthrombi were formed by perfusion of whole blood. This video was recorded over a period of 5 min.
In vitro flow chamber adhesion assays were performed using collagen-coated glass capillaries. Microthrombi were formed by perfusion of whole blood. This video was recorded over a period of 5 min. Download 1.2 Mb. Do'stlaringiz bilan baham: 
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