Francis William Aston won the 1922 Nobel Prize in Chemistry for his work in mass spectrometry Francis William Aston won the 1922 Nobel Prize in Chemistry for his work in mass spectrometry
A continuous dynode particle multiplier detector.
Teacher in the Lab (TIL). Teacher in the Lab (TIL).
What are they? What are they? Where are they found? Why are they important to fisheries biologists?
Examine relationships between otolith isotope signature and length within same species. Examine relationships between otolith isotope signature and length within same species.Compare signatures between species where lengths overlap.Examine variability in signature within fish of a similar size.Examine variability in signature from 2009 to 2010 in Lutjanus synagris of similar sizes.Examine signature variation in Ocyurus chrysurus in similar size fishes from 2010.
Fish take up trace elements by drinkingCertain elements are incorporated into the matrix of otoliths with growth These elements become fixed within the otolith as layers are laid down
10 L. synagris were selected based on size range and collection date from both 2009 and based on size range from 2010 (there was a single collection date in 2010). The total sample size was 20 fish. 10 L. synagris were selected based on size range and collection date from both 2009 and based on size range from 2010 (there was a single collection date in 2010). The total sample size was 20 fish.10 O. chrysurus were selected from each year 2009 and 2010. There was less overlap in sizes available between these collections. The total sample size was 20 fish.Otoliths were extracted, prepared, and examined by mass spectrograph for stable isotopes of oxygen (18O:16O) and carbon (13C:12C).
Otoliths are removed using acid washed glass instruments under clean hoodAll tissue is removed, otoliths washed in ultra-pure waterOtoliths are dried, stored and then transported to AMOL and run through the mass spectrometer
Red L. synagris Red L. synagris Blue O. chrysurus
Red 2009
Boehlert, G.W. and B. C. Mundy. Roles of Behavioral and Physical Factors in Larval and Juvenile Fish Recruitment to Estuarine Nursery Areas. Southwest Fisheries Center Honolulu Labortarory, National Marine Fisheries Service. http://swfsc.noaa.gov/publications/CR/1988/8815.PDF Boehlert, G.W. and B. C. Mundy. Roles of Behavioral and Physical Factors in Larval and Juvenile Fish Recruitment to Estuarine Nursery Areas. Southwest Fisheries Center Honolulu Labortarory, National Marine Fisheries Service. http://swfsc.noaa.gov/publications/CR/1988/8815.PDFStrydom, Nadine A. 2008. Utilization of shallow subtidal bays associated with warm temperate rocky shores by the late-stage larvae of some inshore fish species, South Africa. South African Institute for Aquatic Biodiversity. http://www.bioone.org/doi/pdf/10.3377/1562-7020-43.2.256
Campana, Steven E. 1999. Chemistry and composition of fish otoliths: pathways, mechanisms, and applications. Marine Ecology Progress Series,188:263-297.Radtke, R.L., W. Showers, E. Moksness, and P. Lenz. 1996. Environmental information stored in otoliths: insights from stable isotopes. Marine Biology, 127: 161-170.
Percent = out of 100 % Percent = out of 100 %Relative abundance = out of one wholeAverage Atomic Mass – the average mass of all the naturally occuring isotopes of an element expressed in atomic mass units.
What is average atomic mass of Lithium if 7.42% exists as 6Li (6.015 g/mol) and 92.58% exists as 7Li (7.016 g/mol)? What is average atomic mass of Lithium if 7.42% exists as 6Li (6.015 g/mol) and 92.58% exists as 7Li (7.016 g/mol)? Two isotopes of silver are found using the mass spectrograph. Silver – 107 has an atomic mass of 106.91 amu and an abundance of 51.86 %. The second isotope has an abundance of 48.14 %, but its atomic mass was unable to be determined. What is the atomic mass and the mass number(identity) of this isotope?
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