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Christopher A. Cullis - Plant Genomics and Proteomics-J. Wiley & Sons (2004)

Musa, 32
Musa acuminata, 32
Musa balbisiana, 32
Mutagenesis
gene identification via, 78–87
insertional, 80–82, 83, 84–85, 138
via RNA interference, 80, 86–87
via TILLING, 80, 82–85
transposon, 82, 84
Mutagens, 70


2 1 0
I
N D E X
Mutants, of legumes, 139–141. See also
Mutagenesis
MYB gene family, 97
Mycorrhizal fungi, 138–139
NAC gene family, 97
National Biosafety and Biotechnology
Strategy Plan (Zambia), 194
National Center for Biotechnology
Information (NCBI), 168, 173
databases available from (table), 176
National Plant Genome Initiative, xiv,
66
NDR1 gene, 135–136
Near isogenic lines (NILs), in QTL
identification, 152–155, 160
Nitrogen-fixing bacteria, 138–139
Nonnodulating mutants, of legumes,
139
Nonreciprocal translocations, 14
Normalization, of cDNA libraries, 34
Northern blot, in expression profiling,
109–110
Nuclear DNA, in plants, 1–3
Nuclear isolation, isolating source DNA
via, 28
Nucleic acid searches, 183–185
Nucleolar dominance, 16
Nucleosome, 102
Nucleotide sequences, BLAST
®
and,
175, 176
Nucleotide-binding leucine-rich repeat
(NBS-LRR) genes, 133–134, 136
Oligonucleotides. See also Overgo
entries
as BAC library probes, 30, 31
in microarrays, 110, 112
1C values, 1–3
Open reading frame X (ORFX), in
identifying QTLs, 158–160
Organellar “contamination,” 61
Organ-specific promoters, 148
Orion Genomics, 61
Orthologs, 18
“Overgo oligos,” 30, 31
with BAC contigs, 58
Overgo probes, 58
PaCE clustering tool, 179
PAD4 gene, 136
Paracel TranscriptAssembler
TM
, 179
Paralogs, 18
PAT1:b-glucuronidase (PAT1:GUS)
fusion, 95
Pathogens, xi–xii
plant resistance to, 133–138
PBS2 gene, 136
PCR, real-time, 129
PCR amplification, 151
PCYPAC2N vector, 29
Peptides, sequencing of, 38–39, 40, 121,
122
Peronospora parasitica, 135
Pests, 131
PHD file format, 42
Phenotypes, 19
genes and, 147–149
Phenotypic screening, in marker-
assisted selection, 160–164
Phrap software, 42–43
Phred software, 42–43
Physical mapping, 51–53, 64–65
Physical maps, in genome sequencing,
49, 50–51
Pinus pinaster, DNA content of, 2–3
Pita protein, 137
Plant breeding, bioethics of, 189–190
Plant databases, critique of, 41–42. See
also Databases
PlantGDB database, 179, 180
Plant Genome Research Program, 42, 132
Plant genomes. See also Genomes; Plant
genomics
creating complete sequences of, 47–66
structure of, 1–20
Plant genomics. See also Genomics;
Plant genomes
bioethics of, 189–198
informatics for, 171
rationale for, ix–xiv
Plant roots, interactions of, ix, xi
Plants
abiotic interactions of, 141–144
annotation of genomes of, 77–78
for BAC libraries, 32
biotic interactions of, 132–141


I
N D E X
2 1 1
chromosome variation in, 3–7
complex traits of, 147–164
controlling gene expression in, 89–103
differences between other organisms
and, ix–x
disease resistance of, 131, 132–138
environmental interactions of,
131–144
EST numbers of selected (table), 74
features of, ix
gene spaces of, 66
identifying gene families in, 69–70
proteomics of, 124
recognition of pathogens by, 135
sequence databases for, 65
stress among, 131–132
symbiosis among, 132, 138–141
PlantsP database, 181
PlantsP Kinase Classification (PPC), 
181
Plasmid-based vectors, in cloning,
25–27
Plasmids, 126, 127
Polyacrylamide gel electrophoresis
(PAGE), in protein separation, 124
Polymorphisms, genetic maps and, 51.

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