"Frontmatter". In: Plant Genomics and Proteomics

particular MS technology applied), and the databases ar
e sear
ched to identify the pr
otein. The actual amino
acid sequence is important for those species for which ther
e is little genomic or cDNA
sequence. 
Pr
otein
abundance fr
om sample 1; 
Pr
otein abundance fr
om sample 2.

---

proteins interact. Therefore, an important aspect of proteomics is the isola-
tion of protein complexes and their subsequent characterization to under-
stand the in vivo interactions. 
P
ROTEIN
I
SOLATION
The overall performance of proteome analysis depends on the initial steps,
namely, the isolation of the proteins and the separation tool applied to these
isolated proteins. Plants are differentiated organisms, and many of the spe-
P
R O T E O M I C S
1 2 3
Sample source
Leaves
Roots
Stressed tissue
Sample preparation
Cell disruption
Removal of inhibiting compounds
Solubilization of proteins
Isoelectric Focusing
Separating proteins on the basis of pI
Immobilized pH gradients
Range of pH values
Electrophoresis
SDS gel electrophoresis
Separating proteins on the basis of
molecular weight
Staining
Comassie
Silver
Fluorochromes
Image analysis
Gel comparison
Quantification of
expression levels
Molecular weight and pI
Protein spots from 2DE isolated
Protein degradation
Mass Spectrometry
Peptide mass fingerprinting
Peptide sequencing
Database search
Protein
identification
Bioinformatics
Sequence identified
Known function
Gene identified
Sequence identified
Function unknown
Sequence novel