Integral membrana oqsillari faqat detarjenda

Bakterial porinlar
9
Machine Translated by Google

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Hujayradan tashqari domen salyangozning
atsetilxolin bog'lovchi oqsili bilan gomologikdir
10
teshikka va uchtasi atrofga
Unwin, J.Mol.Biol. 346:967-989 (2005)
Har bir kichik birlik #-spiralga hissa qo'shadi
ACh ning bog'lanishi
15o aylanishga olib
keladi va
membrananing o'rtasida eshikni ochadi
Tabiat 423: 949-955 (2003)
Unwin, J.Mol.Biol. 346:967-989 (2005)
Tabiat 423: 949-955 (2003)
Miyazava, Fujiyoshi va Unwin
Miyazava, Fujiyoshi va Unwin
4A o'lchamdagi kristallografiya
4A o'lchamdagi kristallografiya
Elektron tomonidan aniqlangan struktura
Elektron tomonidan aniqlangan struktura
Nikotinik atsetilxolin retseptorlari
Nikotinik atsetilxolin retseptorlari
Machine Translated by Google

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Balki
translokon
bu qarorni
qiladi?
#-spiral
11
!-varaq
Transmembranani bashorat qilish
Topografiya
IN
OUT
IN
OUT
"Ijobiy-ichkarida" qoidasi
Nilsson va von Heijne
62-uya: 1135-1141 (1990)
Machine Translated by Google

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=O
NH
+N=N- _
NH
-NCS
-C=C- _
CF3
C
O
men*
Reaktiv karbenga CF3
faollashuvi
men*
SO3
C
N=N
NOC-(CH2)4-
NH2-oqsil
SCN-
S
SO3
SO3
men*
_
O
_
_
(DIDS)
(sulfo) NHS-BIOTIN
Trifloro metil-3 yodoarildiazirin
Diazotlangan yodo-sulfanilik kislota
Diizotiosiyanostilben-2,2' disulfonat
(125I-TID)
hidrofobik, membranada eriydi
Yuzaki yorliqlash reagentlari
O
=
12
Antikorlar
Yuzaki yorliqlash
IN
Proteoliz
OUT
..
reaktiv
UV
BIOTIN
Transmembran topografiyasini sinash
SO3
-
-
-
-
Machine Translated by Google

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Oltita transmembran uchun dalil
Lehmann va boshqalar
JBC 272: 12047-51 (1997)
presenilinning domen tuzilishi 1
Transmembran topografiyasini sinash