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Journal of Chemistry of Goods and Traditional Medicine J Chem Good Trad Med, Volume 2, Issue 3, 2023 Traditional Medicine Journal of Chemistry of Goods and Traditional Medicine, Vol. 2, Iss. 3 131 HLILI - (SOLANUM TUBEROSUM) ANALYSIS OF F FLAVONOIDS AND VITAMINS IN PLANTS FOOT (TUSSILAGO FARFARA), CELERY (APIUM GRAVEOLENS), POTATO (SOLANUM TUBEROSUM) Andijon davlat tibbiyot instituti Tibbiy kimyo DOI: https://doi.org/10.55475/jcgtm/vol2.iss3.2023.199 Ushbu maqolada ko ka (tussilago farfara), karafs (apium graveolens), kartoshka (solanum tuberosum) o Technologies- taqqaslash natijalari keltirilgan. Tadqiqot qilingan barcha namunalar tarkibida vitamin B 6 , B 12 hamda flavonoidlardan rutin, kversitin, digidrokversitinga boy bo lganligi uchun suyak, yurak qon tomir, kamqonlik kasalliklarini davolashda yordam beruvchi yangi oziq-ovqat qo shilmalarini yaratish taklif etildi. - - - (solanum 6 , B 12 - Abstract. In this article, flavonoids and vitamins contained in plants -foot (tussilago farfara), celery (apium graveolens) and potatoes (solanum tuberosum) were studied using a high-performance liquid chromatograph Agilent Technologies-1200, and the results of their comparison are presented. Since all the samples studied contain vitamins B 6 , B 12 , as well as flavonoids rich in rutin, quercitin, dihydroxitin, it was proposed to create new dietary supplements that can help in the treatment of bone diseases, cardiovascular diseases and anemia. ko ka (tussilago farfara), (apium graveolens), kartoshka (solanum tuberosum), flavonoidlar, vitaminlar. - Key words. foot (Tussilago farfara), celery (Apium graveolens), potatoes (Solanum tuberosum), flavonoids, vitamins. Kirish. Xalq tabobatida kasalliklarni davolashda shifobaxsh xususiyatlarga ega o simliklar va hayvonlarning tarkibiy qismlari hamda minerallardan foydalanib kelinmoqda [1] Ushbu tabiiy vositalar inson Journal of Chemistry of Goods and Traditional Medicine J Chem Good Trad Med, Volume 2, Issue 3, 2023 Traditional Medicine 132 Journal of Chemistry of Goods and Traditional Medicine, Vol. 2, Iss. 3 1200 ga yaqin turlari dorivorlik xususiyatlariga ega. Hozirgi paytda Respublikamizda 112 turdagi ib, ularning 80% ni tabiiy negizida olib boriladi. Karafs yoki selder. (selderey paxuchiy) apium graveolens L, Petroselinum sativum hoffm L. Avstraliya, Yangi Zelandiya, RF da - Iyulda gullab, avgust- -ket joylashgan. Barg -yumolaq shaklli pista. Gullaganda bargi terib olinib, salqin ab olib, tuproqdan tozalab, suv bilan yuvib, ochiq yerda quritiladi [13]. hosil qiladi, da (optimal - - Efir moyi tarkibida limonen, selinen, sedinolid, shuningdek tarkibi jihatidan gvayanolga yaqin zotiljam, bronxial as jonlantiradigan vosita tarikasida ishlatib kelinadi [3, 29]. Selder ildizlarida 10 dan 20% gacha quruq moddalar, shu jumladan 2-4% shakar, 1-2,5% xom 1% shakar, 2-3% xom protein [14]. Ildizlari ham, barglari ham askorbin kislota (barglarda bu vitaminning 110 mg% gacha), Selderning barcha qismlari birinchi va ikkinchi ovqatlarga, salatlarga, ichimliklarga, adi. Ildizpoya ham quritilgan holda ishlatiladi. Poyalarini buyraklar, Karafs. Selder. (selderey paxuchiy) apium graveolens L, Petroselinum sativum hoffm L. 3 mahal 50 ml dan ovqatdan yarim soat oldin ichiladi. [1, 487-bet] -navbat joylashgan, toq patsimon. Gullari Journal of Chemistry of Goods and Traditional Medicine J Chem Good Trad Med, Volume 2, Issue 3, 2023 Traditional Medicine Journal of Chemistry of Goods and Traditional Medicine, Vol. 2, Iss. 3 133 oq, och pushti, pushti- - -avgustda gullaydi. martda) va kechki kartosh kalar (may oxiri iyunda) ekiladi. Kartoshkani Rossiyada 18- - asrning birinchi yarmida ekila boshlangan. Kartoshkaning B, B1, PP, C - ham borligi aniqlangan [19]. i machexa. Tu - - tuk, oqish yuqori tomoni esa, yashil, tuksiz [8]. uchmali pista. -may oylarida gullaydi. Mevasi may- sh oldida gkli savatchasi bilan bandsiz qirqib olinadi. Bargi yoz oylarida barg n, vitamin S, organik kislotalar, efir moyi, shilliq, oshlovchi va boshqa moddalar bor [9]. shamollash, sil kasalligini davolashda ishlatiladi. Nafas siqish paytida, uning quritilgan barglari papiros kabi chekilganda, juda dorilar ishlab chiqari 12% tanid, glyukozid, saponin, tusilagin, fitostrin, inulin, kraxmal, karotinoid moddalari hamda S 0,12 %efir moyi, tanid va Tajriba qism. Suvda eruvchi vitaminlar miqdori yuqori samarali suyuqlik xromotografiyasi usulida suyuqlik xromatografiyasi usuli yordamida aniqlandi. Tortmadan 5-10 gr miqdorida analitik tarozida tortib bilan jihozlanib, 1 soat davomida intensiv aralashtirib turgan holda qaynatiladi va keyinchalik 2 soat davomida xona haroratida aralashtiriladi. ekstraktsiya - analiz uchun olindi. Suvda eruvchan vitaminlar 1mg/ml kontsentratsiyali ishchi eritmalari tayyorlab olindi. Buning uchun har bitta vitamin standartidan analitik tarozida 50,0 mg aniq tortma tortib olinadi va Journal of Chemistry of Goods and Traditional Medicine J Chem Good Trad Med, Volume 2, Issue 3, 2023 Traditional Medicine 134 Journal of Chemistry of Goods and Traditional Medicine, Vol. 2, Iss. 3 atsetatli bufer sistemalari va atsetonitrildan foydalanilgan. Biz atsetatli bufer sistemasi hamda atsetonitrildan foydalandik. Xromotografiya sharoitlari: -Xromotograf Agilent-1200 (avtodozator bilan jihiozlangan) -Kolonka Exlipse XDB C 18, 5 mkm, 4,6 x150mm -Diod matritsali detektor (DMD), 265nm, 254 nm, 290 nm identifikatsiya qilindi. -Oqim tezligi 1ml/min - Elyuent atsetatli bufer: atsetonitril: 0-5 min 96:4, 6-8 min 90:10, 9-15 min 80:20, 15-17 min 96:4, termostat harorati 250 0 -5 mkl kiritilgan miqdor (vkol) Xromotografga dastlab, ishchi standart eritmalar, keyinchalik tayyorlangan ishchi eritmalar kiritildi. 1-rasm. Standart eritmalar xromatogrammasi. 2-rasm. Selderey bargining suvda eriydigan vitaminlar xromatogrammasi. Journal of Chemistry of Goods and Traditional Medicine J Chem Good Trad Med, Volume 2, Issue 3, 2023 Traditional Medicine Journal of Chemistry of Goods and Traditional Medicine, Vol. 2, Iss. 3 135 3-rasm. Sel`derey urug'ining suvda eriydigan vitaminlar xromatogrammasi. 4-rasm. Ko'ka bargining suvda eriydigan vitaminlar xromatogramasi. 5-rasm. Kartoshka po'stining suvda eriydigan vitaminlar xromatogrammasi. Namunalar tarkibida 6 xil suvda eriydigan vitaminlar borligi aniqlandi va analiz natijalari 1- jadvalda keltirildi. 1-jadval. Analiz natijasida olingan namunalar tarkibidagi vitaminlar miqdori Vitaminlar Karafs bargi Karafs urug'i Kartoshka po'sti Ko'ka bargi Konsentratsiya mkg/gr -1 0,11 0 0,02348 0 -12 6,3473 0,229715 1,726287 0,256434 -6 19,833 1,299861 3,599237 1,226006 Journal of Chemistry of Goods and Traditional Medicine J Chem Good Trad Med, Volume 2, Issue 3, 2023 Traditional Medicine 136 Journal of Chemistry of Goods and Traditional Medicine, Vol. 2, Iss. 3 -9 0,6596 1,440369 0,257036 0,756557 -3) 0,0778 0 0 0 0 0,025844 0,013588 0 Jadvaldan ko'rinib turibdiki, vitamin PP faqat selder bargida oz miqdorda aniqlangan qolgan namunlarda deyarli yo'q. Vitamin C esa faqat selder urug'i va kartoshka po'stida oz miqdorda qolgan namunlarda esa umuman aniqlanmagan. Lekin vitamin B12 va B6 boshqa barcha namunalar tarkibiga nisbatan kontsentratsiyasi sezilarli darajada ko'pligini ko'rishimiz mumkin. B6 vitamini o'zining koenzimlari shaklida aminokislotalarning oldindan aylanishida, triptofan, lipidlar va nuklein kislotalarning metabolizmida, immunitetni saqlashda, markaziy asab tizimidagi qo'zg'alish jarayonlarida ishtirok etadi, eritrotsitlarning normal shakllanishiga, qonda homosisteinning normal darajasini saqlashga yordam beradi [19]. B6 vitaminini etarli darajada iste'mol qilmaslik ishtahaning pasayishi, terining holati buzilishi bilan birga keladi, homosisteinemiya, anemiya rivojlanishi. B12 vitamini aminokislotalarning metabolizmi va o'zgarishida muhim rol o'ynaydi. Folat va B12 vitamini qon hosil bo'lishida ishtirok etadigan o'zaro bog'liq vitaminlardir. B12 vitaminining etishmasligi qisman rivojlanishiga olib keladi yoki ikkilamchi folat etishmovchiligi, shuningdek anemiya, leykopeniya, trombotsitopeniya [37]. Vitamin B9 yoki folat etishmovchiligi nuklein kislotalar va oqsil sintezining buzilishiga olib (suyak iligi, ichak epiteliyasi va boshqalar). . Namunadan 5-10 gr miqdorida analitik tarozida tortib olinib, 300 ml hajmdagi yassi kolbaga solinadi. Ustiga 50 ml 70% li etanol eritmasidan qo'shiladi. Aralashma magnit aralashtirgich, teskari sovutkich bilan jihozlanib, 1 soat davomida intensiv aralashtirib turgan holda 70-800 C qaynatiladi va keyinchalik 2 soat davomida xona haroratida aralashtiriladi. Aralashma tindirilib fil`trlab olinadi. Qolgan qismiga 25 ml 70 % etanoldan solib 2 marta qayta ekstraktsiya qilinadi. Fil`tratlar birlashtiriladi va 100 ml o'lchagich kolbaga solinib chizig'igacha 70% etanol bilan to'ldiriladi. Hosil bo'lgan eritma tsentrafugada 6000-8000 oborot/minut tezlikda 20-30 daqiqa davomida aylantiriladi. Hosil bo'lgan eritma ustki qismidan analiz uchun olindi. Xromotografga dastlab, ishchi standart eritmalar, keyinchalik tayyorlangan ishchi eritmalar kiritildi. 6-rasm. Sel`derey urug'i xromatogrammasi. Journal of Chemistry of Goods and Traditional Medicine J Chem Good Trad Med, Volume 2, Issue 3, 2023 Traditional Medicine Journal of Chemistry of Goods and Traditional Medicine, Vol. 2, Iss. 3 137 7-rasm. Selder bargi xromatogrammasi 8-rasm. Kartoshka po'sti xromatogrammasi 9-rasm. Ko'ka bargi xromatogrammasi Namunalar ekstraktlarini tahlil qilish natijasida oltita fenollik birikma-flavonoidlar borligi aniqlandi. Tahlil natijalari 2-jadvalda keltirilgan. Jadval-2. Analiz natijasida olingan namunalar tarkibidagi flavonoidlar miqdori O'simliklar tarkibidagi flavonoidlar miqdori Selder bargi Selder urug'i Ko'ka bargi Kartoshka po'sti Kontsentratsiya mg/gr Apiginin 0 0 0 0 Lyutionin 0,62 0,24 1,12 1.12 Rutin 2,32 1,52 2,65 2.65 Kvertsitin 1,65 0,95 3,25 3.25 Journal of Chemistry of Goods and Traditional Medicine J Chem Good Trad Med, Volume 2, Issue 3, 2023 Traditional Medicine 138 Journal of Chemistry of Goods and Traditional Medicine, Vol. 2, Iss. 3 Digidrokvertsetin 3,97 1,64 2,98 2.98 Senerozid 1,59 0,69 0 0 Ushbu o'simliklardagi flavonoidlar tarkibini tahlil qilish shuni ko'rsatadiki, flavonoidlar rutin, kvertsitin, digidrokvertsetinning mavjudligi va nisbatan yuqori miqdori bu o'simliklarni o'rganishga qiziqishni yanada oshiradi. Lyutionin boshqa namunalarga qaraganda ko'ka o'simligi bargida nisbatan borligi aniqlanildi. Senerozid esa faqat selderda mavjudligi taxlil qilindi. Kvartsetin bir qator biologik faol qo'shimchalar va dorilarning bir qismidir, u muqobil tibbiyotda ham qo'llaniladi. Jadval shuni ko'rsatadiki, rutin (mg/g) kontsentratsiyasi barcha namunalarda ancha yuqori. ishlab chiqarishni rag'batlantiradi va kollagen sintezini faollashtiradi, uning asosida artikulyar xaftaga hosil bo'ladi. Boshqa flavonoidlar bilan bir qatorda rutin qon tomir devorlarini mukammal darajada mustahkamlaydi, ularning elastikligini oshiradi, bu qon tomirlarining o'tkazuvchanligini yaxshilash va qon oqimini tezlashtirish uchun muhimdir. Masalan, artrit, osteoartrit, osteoxondroz bilan qon oqimi patologik sekinlashadi, shuning uchun bo'g'inlar va umurtqa pog'onasining oziqlanishi buziladi. [18] Digidrokersetin suyaklarning mustahkamligi uchun zarur bo'lgan biriktiruvchi to'qimalarda kollagen va elastin ishlab chiqarishni normallashtirishda ishtirok etadi. DGK ning qon oqimini tezlashtirish qobiliyati bo'g'inlar va umurtqa pog'onasining holatini yaxshilaydi, shuningdek harakatlarning amplitudasini oshiradi. Digidrokersetinni radikallarni tanadan chiqarib yuborish, tanadagi sog'lom zarrachalarning ustunligini o'rnatish va immunitetni oshirishning yorqin qobiliyati bilan izohlanadi. Xulosa. Tadqiqotlar davomida yuqoridagi o'simliklardagi flavonoidlar hamda suvda eriydigan vitaminlari o'rganildi va maqolada taxlil qilindi. O'simlikllarni kompleks taxlil qilish orqali xalq tabobatida umurtqa churrasi kasalliklarni oldini olishda foydalaniladigan yangi oziq-ovqat qo'shimchalarini yaratish maqsadga muvofiq. - . - Journal of Chemistry of Goods and Traditional Medicine J Chem Good Trad Med, Volume 2, Issue 3, 2023 Traditional Medicine Journal of Chemistry of Goods and Traditional Medicine, Vol. 2, Iss. 3 139 - - - - - - 1- - - - - - - - - - - Journal of Chemistry of Goods and Traditional Medicine J Chem Good Trad Med, Volume 2, Issue 3, 2023 Traditional Medicine 140 Journal of Chemistry of Goods and Traditional Medicine, Vol. 2, Iss. 3 - - Tussilago farfara - 5- - - - - - - 0,08 - - - - - - - - - - Journal of Chemistry of Goods and Traditional Medicine J Chem Good Trad Med, Volume 2, Issue 3, 2023 Traditional Medicine Journal of Chemistry of Goods and Traditional Medicine, Vol. 2, Iss. 3 141 0- 6- :10, 9- 15- 0 - - Journal of Chemistry of Goods and Traditional Medicine J Chem Good Trad Med, Volume 2, Issue 3, 2023 Traditional Medicine 142 Journal of Chemistry of Goods and Traditional Medicine, Vol. 2, Iss. 3 - - - - -1 0,11 0 0,02348 0 -12 6,3473 0,229715 1,726287 0,256434 -6 19,833 1,299861 3,599237 1,226006 -9 0,6596 1,440369 0,257036 0,756557 -3) 0,0778 0 0 0 0 0,025844 0,013588 0 Journal of Chemistry of Goods and Traditional Medicine J Chem Good Trad Med, Volume 2, Issue 3, 2023 Traditional Medicine Journal of Chemistry of Goods and Traditional Medicine, Vol. 2, Iss. 3 143 - - - - - Journal of Chemistry of Goods and Traditional Medicine J Chem Good Trad Med, Volume 2, Issue 3, 2023 Traditional Medicine 144 Journal of Chemistry of Goods and Traditional Medicine, Vol. 2, Iss. 3 - - - -2. - - 0 0 0 0 0,62 0,24 1,12 1.12 2,32 1,52 2,65 2.65 1,65 0,95 3,25 3.25 3,97 1,64 2,98 2.98 1,59 0,69 0 0 - - Journal of Chemistry of Goods and Traditional Medicine J Chem Good Trad Med, Volume 2, Issue 3, 2023 Traditional Medicine Journal of Chemistry of Goods and Traditional Medicine, Vol. 2, Iss. 3 145 Introduction. In folk medicine, components of plants and animals are used to treat diseases, as well as minerals with healing properties [1.] The fact that these natural remedies do not have a negative effect on the human body has been proven in long-term experiments. On the other hand, the integration of modern medicine and traditional medicine in the treatment of patients is becoming increasingly important to prevent many problems [1,2]. On the territory of Uzbekistan, about 4,500 species of higher plants are distributed in their natural form, of which about 1,200 species have medicinal properties. Currently, 112 types of medicinal plants are allowed to be used in official medicine in our republic, of which 80% are plants growing in a natural state [3,4]. The ancient method of treating diseases is folk medicine, in which the treatment of diseases is carried out on the basis of medicinal plants, animals, minerals and many other natural elements. Below is an analysis of medicinal plants for flavonoids and vitamins used by us in the treatment of hernial diseases of the spine. Celery. Apium Graveolens L, Petroselinum sativum Hoffm L. In nature, wild celery is common in Western Europe, Asia, India, North and South America, Australia, New Zealand, the Russian Federation - mainly in the south of the European part. The plant is grown almost all over the world. In Uzbekistan, it grows on the irrigated lands of Tashkent, Samarkand, Namangan, Andijan, Fergana, Kashkadarya, Surkhandarya, along the banks of canals, streams, at the edges of reservoirs, in wet places [1,3]. Blooms in July, ripens in August-September. A perennial herbaceous plant up to 50 cm tall.. The stem is erect, hollow inside, branched, starting from the middle part. The leaves of the basal and lower part of the stem are long-stemmed, pinnately dissected. The tops of the stems are three-lobed, trimmed, arranged in a row. The leaf blades are oblong or lanceolate. The flowers are small, white, collected in a complex umbrella. The fruit is a round-shaped pistachio. When flowering, the leaf is collected and dried in a cool place. The aboveground part is mowed when the fruits begin to ripen. The roots are dug out in autumn, cleaned from the soil, washed with water and dried in the open ground [13]. The fruits contain essential oil, flavanoids, fats, the leaf contains essential oil, carotene, vitamins C, B, glycoside apinin and other flavonoids. In the first year it forms leaves and roots, in the second year it blooms. The plant is moisture-loving and frost- frosts up to - The essential oil contains limonene, selinene, sedinolide, as well as phenols, which are similar in composition to guianol. Furocoumarins and a significant amount of fatty oil were found in the Journal of Chemistry of Goods and Traditional Medicine J Chem Good Trad Med, Volume 2, Issue 3, 2023 Traditional Medicine 146 Journal of Chemistry of Goods and Traditional Medicine, Vol. 2, Iss. 3 fruits. The leaves contain carotene, vitamin C and a glycoside called apinin [12]. In Central Asian folk medicine, fiber was used to treat pneumonia, bronchial asthma, hepatitis, abdominal cramps, hiccups, back pain, as well as as a motor agent that removes urine, bile and iodine, stops vomiting, revives the central nervous system [3, 29]. Celery roots contain from 10 to 20% dry matter, including 2-4% sugar, 1-2.5% crude protein, potassium, calcium, phosphorus; leaves contain from 10 to 18% dry matter, including about 1% sugar, 2-3% crude protein [14]. Both roots and leaves are rich in ascorbic acid (the leaves contain up to 110 mg% of this vitamin), carotene (provitamin A), vitamins B1, B2, vitamin PP, potassium, calcium, phosphorus salts [15]. All parts of celery are added to the first and second courses, salads, drinks, sauces, seasonings. The rhizome is also used in dried form. Stems are recommended to be used instead of salt in diseases of the kidneys, gallbladder, osteoporosis, osteochondrosis [16]. Celery contains biologically active substances that have a positive effect on metabolism, hair and skin condition. Antioxidants in the plant slow down aging, essential oils help the work of the intestine and have an antibacterial effect [17]. Celery. Apium Graveolens L, Petroselinum sativum Hoffm L. In cases of osteochondrosis, root juice should be consumed 2 teaspoons 3 times a day. A tablespoon of the root is poured with two glasses of boiling water and infused for 4 hours. Drink 50 ml 3 times a day half an hour before meals. [1, p. 487] Potato. Salanum tuberosum L. Potato is a perennial herbaceous tuberous plant reaching 80 cm in height. The leaves are alternate, five-lobed. The flowers are white, light pink, pink-blue. The fruits are large-large, spherical, green in color. Blooms in June-August. Potatoes are native to South America, from where they were brought to Europe. In Uzbekistan, early (late February March) and late potatoes are sown (late May June). Potatoes began to be planted in Russia at the beginning of the 18th century, and in Uzbekistan - in the first half of the 19th century. The underground potato tuber is edible. This plant is mostly planted everywhere because of its nodes, which are used for food. There are many varieties, nodes are used [10]. Potatoes contain 2% proteins, carbohydrates, fats, citric and malic acids, vitamin B, B1, PP, C and other substances. Compared to other plants, potatoes contain the most starch -17.5%. When many nodules remain in the light for too long, a toxic steroid aocaloid called solanine is formed in them. It was found that potato leaves with niches also contain 6 different glycoalkaloids [19]. Tussilago farfara is a perennial herbaceous plant, reaching 5-25 cm in height. In early spring, several unbranched stems grow from the rhizome, forming flowers. The stem is covered with red- brown scaly leaves with an inflorescence at the end. The stem dries up after the fruit ripens. Then long striped basal leaves grow. The basal tubers are rounded or broadly ovate, with unevenly cut serrated edges and long stripes, the underside of the leaf plate is grayish, and the upper side is whitish, green, naked [8]. The flowers are golden yellow, collected in baskets. The fruit is a triple pistachio. Blooms in April-May. The fruits ripen in May-June. Collect a leaf and a blueberry flower. Before flowering, the plant is cut off without strapping with a basket of gcli. In the summer months, the leaf breaks off from half of the leaf strip. The collected flowers and leaves are thinly laid out in cool ground and dried.the plant contains tussilagin and other bitter glycosides, saponins, carotene, vitamin C, organic acids, essential oil, mucus, astringent and other substances [9]. It is common in meadows, along the banks of streams and rivers. Decoction, juice and aphids made from the leaves of the white-tailed beetle are used to treat various diseases: cough, colds, tuberculosis. With shortness of breath, when its dried leaves are smoked like cigarettes, it gives a very good result. [11]. In the preparation of medicines, the leaf of the plant is included in the composition of tea and is used in the manufacture of galenic medicines. This plant is rich in various substances. It Journal of Chemistry of Goods and Traditional Medicine J Chem Good Trad Med, Volume 2, Issue 3, 2023 Traditional Medicine Journal of Chemistry of Goods and Traditional Medicine, Vol. 2, Iss. 3 147 contains 5-12% tannide, glucoside, saponin, tusilagin, phytostrin, inulin, starch, carotenoids, as well as a lot of vitamin C. Also, henbane flower contains 0.08 0.12% essential oil, tanide and taraxanthin dye [30]. Experimental part. The content of water-soluble vitamins was studied by high-performance liquid chromatography. Water-soluble vitamins in the sample were detected using a highly efficient liquid chromatography method. 5-10 grams are taken out of the box on the analytical scales and placed in a flat flask with a volume of 300 ml. 50 ml of 40% ethanol solution is added on top. The mixture is boiled using a magnetic blender, a reverse cooler, stirring intensively for 1 hour, and then stirred at room temperature for 2 hours. The mixture is sifted and filtered. The remaining part was re-extracted 2 times, adding 25 ml of 40% ethanol. The filtrates are combined and placed in a 100 ml volumetric flask, filled with 40% ethanol (5-10%). The resulting solution is rotated in a centrifuge at a speed of 7000 revolutions per minute for 10 minutes. The resulting solution was taken for analysis from above. Working solutions of water-soluble vitamins with a concentration of 1 mg/ml were prepared. To do this, 50.0 mg of a clean box is taken out of each vitamin standard on analytical scales, and 50 ml is dissolved in a measuring flask in 40% ethanol and filled to the mark. In the literature, phosphoric, acetate buffer systems and acetonitrile were used as eluents in the determination of water-soluble vitamins using YuSSX. We used an acetate buffer system, as well as acetonitrile. Chromatography conditions: - Agilent-1200 chromatograph (auto-dispenser) - Column Exlipse XDB C 18, 5 microns, 4.6 x150mm - Detector with diode array (DDA), 265nm, 254 nm, 290 nm. - Flow rate 1 ml/min - Eluent acetate buffer: acetonitrile: 0-5 min 96:4, 6-8 min 90:10, 9-15 min 80:20, 15-17 min 96:4, thermostat temperature 2500 C, - Initially, working standard solutions were introduced into the chromatograph, then prepared working solutions. Figure 1. Chromatogram of standard solutions. Journal of Chemistry of Goods and Traditional Medicine J Chem Good Trad Med, Volume 2, Issue 3, 2023 Traditional Medicine 148 Journal of Chemistry of Goods and Traditional Medicine, Vol. 2, Iss. 3 Figure 2. Chromatogram of water-soluble vitamins of celery leaf. Figure 3. Chromatogram of water-soluble vitamins of celery seeds. Figure 4. Chromatogram of water-soluble vitamins of the mother-and-stepmother leaf. Journal of Chemistry of Goods and Traditional Medicine J Chem Good Trad Med, Volume 2, Issue 3, 2023 Traditional Medicine Journal of Chemistry of Goods and Traditional Medicine, Vol. 2, Iss. 3 149 Figure 5. Chromatogram of water-soluble vitamins of peels of potato. It was found that the samples contain 6 different water-soluble vitamins, and the results of the analysis are presented in Table 1. Table 1. The amount of vitamins in the samples obtained as a result of the analysis Vitamins Leaves of celery Seeds of Celery Peel of potato Leaves of foalfoot Concentration of mcg/g -1 0,11 0 0,02348 0 -12 6,3473 0,229715 1,726287 0,256434 -6 19,833 1,299861 3,599237 1,226006 -9 0,6596 1,440369 0,257036 0,756557 -3) 0,0778 0 0 0 0 0,025844 0,013588 0 As can be seen from the table, vitamin PP is found only in celery leaf in small quantities, in other samples it is practically absent. Vitamin C, on the other hand, is not found at all in samples that remain in small amounts only in celery seeds and potato skins. But we can see that the concentration of vitamin B12 and B6 is significantly higher in all samples. Vitamin B6 in the form of coenzymes participates in the preliminary amino acid cycle, in the metabolism of tryptophan, lipids and nucleic acids, in maintaining immunity, in the processes of excitation in the central nervous system, contributes to the normal formation of red blood cells, maintaining normal levels of homocysteine in the blood [19]. Insufficient intake of vitamin B6 is accompanied by a decrease in appetite, skin disorders, the development of homocysteinemia, anemia. Vitamin B12 plays an important role in the metabolism and conversion of amino acids. Folic acid and vitamin B12 are interrelated vitamins involved in hematopoiesis. Vitamin B12 deficiency leads to partial development or secondary folic acid deficiency, as well as anemia, leukopenia, thrombocytopenia [37]. Deficiency of vitamin B9 or folic acid leads to disruption of the synthesis of nucleic acids and protein, which ultimately affects the growth and division of cells, especially in rapidly multiplying tissues (bone marrow, intestinal epithelium, etc.). The flavonoids in the sample were determined by liquid chromatography. 5-10 grams are taken out of the sample on analytical scales and placed in a flat flask with a volume of 300 ml. 50 ml of 70% ethanol solution is added on top. The mixture is boiled at 70-800 C with intensive stirring for 1 hour, equipped with a magnetic stirrer, a reverse cooler, and then stirred at room temperature for 2 hours. The mixture is infused and filtered. The remaining part is re-extracted 2 times, adding 25 ml of 70% ethanol. The filtrates are mixed and placed in a 100 ml volumetric flask filled with 70% Journal of Chemistry of Goods and Traditional Medicine J Chem Good Trad Med, Volume 2, Issue 3, 2023 Traditional Medicine 150 Journal of Chemistry of Goods and Traditional Medicine, Vol. 2, Iss. 3 ethanol. The resulting solution is rotated in a centrifuge at a speed of 6000-8000 revolutions per minute for 20-30 minutes. The resulting solution was taken for analysis from above. Initially, working standard solutions were introduced into the chromatograph, then prepared working solutions. Figure 6. Chromatogram of seeds of celery. Figure 7. Chromatogram of leaves of celery Figure 8. Chromatogram of peel of potato Journal of Chemistry of Goods and Traditional Medicine J Chem Good Trad Med, Volume 2, Issue 3, 2023 Traditional Medicine Journal of Chemistry of Goods and Traditional Medicine, Vol. 2, Iss. 3 151 Figure 9. Chromatogram of leaves of foalfoot Analysis of the extracts of the samples showed the presence of six phenolic compounds- flavonoids. The results of the analysis are presented in Table 2. Table-2. The amount of flavonoids in the samples obtained as a result of the analysis Flavonoids Leaves of celery Seeds of celery Leaves of foalfoot Peel of potato Concentration mg/g Apigenin 0 0 0 0 Lutyonin 0,62 0,24 1,12 1.12 Routine 2,32 1,52 2,65 2.65 Quercitin 1,65 0,95 3,25 3.25 Dihydroquercetin 3,97 1,64 2,98 2.98 Seneroside 1,59 0,69 0 0 Analysis of the content of flavonoids in these plants shows that the presence and relatively high content of flavonoids rutin, quercitin, dihydroquercetin further increases interest in the study of these plants. It was found that luthionine is relatively present in mother-and-stepmother leaves compared to other samples. However, it was analyzed that ceneroside is present only in celery. Quartzetin is a part of a number of biologically active additives and drugs, it is also used in alternative medicine. The table shows that the concentration of rutin (mg/g) in all samples is quite high. It is known that rutin protects the hyaluronic acid molecule from destruction, stimulates the production of synovial lubrication and activates the synthesis of collagen, on the basis of which articular cartilage is formed. Along with other flavonoids, rutin perfectly strengthens the walls of blood vessels, increases their elasticity, which is important for improving vascular permeability and accelerating blood flow. For example, with arthritis, osteoarthritis, osteochondrosis, blood flow is pathologically slowed down, so the nutrition of the joints and spine is disrupted. [18] Dihydroquercetin is involved in normalizing the production of collagen and elastin in connective tissue, which are necessary for bone strength. The ability of DHQ to accelerate blood flow improves the condition of the joints and spine, and also increases the amplitude of movements. The anti-inflammatory effect of dihydroquercetin is explained by its brilliant ability to enhance microcirculation, remove free radicals from the body, establish the predominance of healthy particles in the body and increase immunity. Journal of Chemistry of Goods and Traditional Medicine J Chem Good Trad Med, Volume 2, Issue 3, 2023 Traditional Medicine 152 Journal of Chemistry of Goods and Traditional Medicine, Vol. 2, Iss. 3 Conclusion. In the course of research, flavonoids, as well as water-soluble vitamins in the above-mentioned plants were studied and analyzed in the article. It is advisable to create new food additives used in folk medicine for the prevention of diseases of vertebral hernias through a comprehensive analysis of plant components. References: 1. 2019. 2. 3. - . 4. 3- 2000 5. 6. . Tussilago farfara L. - - // 7. , , , - 520 ISBN 5-87317-163-7. 8. - - Rubiaceae Compositae 2000. 54 61. 179. 411 9. - - 391. ( : ; 2004 19). ISBN 978-5-85270-353-8. 10. - 11. Senecioneae Cass. (Asteraceae zaninowia : 2000. . 63. 581.9 (571.1/5) : 582.998 2010) 12. 2018. -40. 13. - - 2000. - 14. - 2- - 15. -yil. X.X Xalmatov 16. -yil. 17. - . 18. - 2013 259-260- . 19. ISBN 978-5-9986-0234- 20. 2004. 21. 2000. Journal of Chemistry of Goods and Traditional Medicine J Chem Good Trad Med, Volume 2, Issue 3, 2023 Traditional Medicine Journal of Chemistry of Goods and Traditional Medicine, Vol. 2, Iss. 3 153 22. - 2016.- 23. - 24. - 25. - 2000 - 26. . 2000 27. 1999 28. - 29. Muxtorov M. -23 betlar. 30. . ., . . ,1992 . 50- 31. 32. 33. (1990). 34. 35. 36. 37. 38. - 39. 40. - 41. - 42. - 43. Author Biography Saidaxon T. Islomova, Andijan State University Associate Professor of the Department of Medical Chemistry, Doctor of Philosophy in Technical Sciences Download 4.8 Mb. 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