P-issn: 2349-8234 jpp 2017; 6(2): 10-16 Received: 01-01-2017 Accepted: 02-02-2017 Kiran a wadkar

 
 
Fig 3: HPTLC fingerprint of Cystone formulation along with marker 
compounds. 
1. CS-Catechin Standard, 2. IR- Isolated from Rhizome of 
berginia ligulata, 3. IF- Isolated from Cystone formulation, 4. 
Cys F- Cystone Formulation Extract. 
 
Fig 4: HPTLC chromatogram of Standard Catechin. 
 
Fig 5: HPTLC chromatogram of Isolated Catechin.
The standard HPTLC chromatogram Standard Catechin and 
Isolated Catechin showed two peaks with Rf value 0.76 and 
0.82. (Figure. 4 and 5)
 
9. Characterization of extract of Cystone formulation by 
HPLC 
The HPLC analysis of standard Catechin and Isolated 
Catechin shows peaks with retention times of 2.773 and 2.773 
min, respectively, (Figure. 6 and 7). 
 
Fig 6: HPLC profile of standard Catechin. 
 
Fig 7: HPLC profile of Isolated Catechin from Formulation
. 
Thus, it can be said that extract contain catechin. It can be the 
possible analytical markers for standardization of Cystone 
formulation. After quantitative estimation of catechin 
specifications can be stated. This should then serve as a 
simple, accurate and routine method of analysis for Cystone 
formulations. 
10. Conclusion 
The spectral data and HPTLC fingerprint of ethanolic extract 
of Cystone formulation could be used as a valuable analytical 
tool in the routine standardization of Cystone formulation to 
check the batch to bath variation. Catechin can be used as one 
of the appropriate analytical markers for standardization of 
Cystone formulation. 
11. Acknowledgements 
This work is financially supported by University Grants 

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Journal of Pharmacognosy and Phytochemistry
Commission, New Delhi under Major Research Project [Grant 
No.42-708/2013(SR)]. Authors are also thankful to Principal 
D. D. Chougule of Appasaheb Birnale college of Pharmacy 
Sangli for providing the necessary facilities to carry out this 
work.