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Overview, continued
Recommended
Equipment
Below is a checklist for equipment recommendations.
• A jacketed vessel is needed for cooling the yeast during fermentation, especially
during methanol induction. You will need a constant source of cold water (5-10°C).
This requirement may mean that you need a refrigeration unit to keep the water cold.
• A foam probe is highly recommended as antifoam is required.
• A source of O
2
--either air (stainless steel fermenters at 1-2 vvm) or pure O
2
(0.1-
0.3 vvm for glass fermenters).
• Calibrated peristaltic pumps to feed the glycerol and methanol.
• Automatic control of pH.
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