Research of natural honey of group 04 of the commodity nomenclature of foreign economic activity: identification examination and physic-chemical properties

OBJECTS AND METHODS OF RESEARCH
The objects of the study are the classification features of natural and artificial honey. When performing research work on the classification of natural honey according to the commodity nomenclature, an analytical research method was used[9,10]. This method is used to substantiate the necessary norms in the conditions of the current production based on observations and experiments. Based on the data received, relevant standards are being developed. The high complexity of collecting primary information leads to the use of this method for the development of various regulatory materials. To determine proline in honey, a method for its determination has also been developed. According to the methodology, solutions for testing are prepared as follows:
To prepare a standard solution of L-proline, 15.7 mg of L-proline (accurately weighed) are weighed into a 100 ml volumetric flask, then it is dissolved in water, the volume is adjusted to the mark and mixed.
Pour 10 ml of the solution into a 25 ml volumetric flask, dilute to the mark with water and mix. [11].
2 ml of the obtained solution is poured into a volumetric flask with a capacity of 25 ml, 2 ml of a saturated sodium bicarbonate solution, 1 ml of a derivatizing agent are added, mixed well. Derivatize this solution (Place the pre-filtered desired sample in a 60°C water bath for 1 hour with frequent stirring), then cool, bring the volume to the mark with pH 7.0 phosphate buffer, and mix thoroughly[12,13].
To prepare the test solution, 7000 mg of a selected sample of honey is placed in a glass, dissolved in water until dissolved, transferred to a 50 ml volumetric flask, the volume of the solution is adjusted to the mark with water and mixed well.
5 ml of the resulting solution is transferred to a 25 ml volumetric flask, 2 ml of a saturated sodium bicarbonate solution, 1 ml of a derivatizing agent are added, mixed well. Derivatize as indicated in the method, cool, bring the volume to the mark with phosphate buffer pH=7.0 and mix thoroughly.
Conditions for chromatographic analysis:
The test is carried out on an Agilent Technologies 1200 high performance chromatograph or equivalent.
- a column with a size of 4.6 mm x 15 cm, filled with a sorbent Zorbax Eclipse XDB C-18, with a particle size of 5 microns, or similar;
- wavelength 361 nm;
- sample volume 20 µl.
The ratio of mobile phases along the gradient is given in the following table:

Table 1