Clin Biochem. 2010 Apr;43(6):549-52. Epub 2010 Feb 2. Study
Download 5.37 Mb.Pdf ko'rish
Clin Biochem. 2010 Apr;43(6):549-52. Epub 2010 Feb 2.
Polymorphisms in Type 2 Diabetes and Obesity Risk in the
Bouhaha R, Baroudi T, Ennafaa H, Vaillant E, Abid H, Sassi R, Vatin V,
Froguel P, Gaaied AB, Meyre D, Vaxillaire M.
Laboratory of Genetics, Immunology and Human Pathologies, Faculty of
Sciences of Tunis, 2092 Tunis, Tunisia. firstname.lastname@example.org
molecules : TNFalpha -308G/A and IL6 -174G/C in order to assess their
eﬀect on type 2 diabetes (T2D) and obesity in the Tunisian popula on.
and 300 healthy controls. Genotyping of IL6 -174G/C (rs1800795) was
performed using Automated Dye Terminator Sequencing and of TNFalpha -
308G/A (rs1800629) using the LightTyper technology.
RESULTS: SNPs IL6 -174G/C and TNFalpha -308G/A are associated neither
with T2D (p=0.89, p=0.34 respec vely) nor with risk for overweight (p=0.86,
p=0.12 respectively) in Tunisian population. Bonferroni correction showed
that the founded associa on of IL6 -174G/C SNP with T2D suscep bility
restricted to overweight pa ents (p(nominal)=0.03, p(corrected)=0.0033) is
likely to be a random result.
CONCLUSION: SNPs IL6 -174G/C and TNFalpha -308G/A are not major
contributors to T2D or obesity risk in our Tunisian popula on.
Int J Obes (Lond). 2010 Feb;34(2):273-9. Epub 2009 Dec 15.
Obesity-Induced Impairment of Endothelium-Dependent
Vasodilation in Tunisian Women.
Miâdi-Messaoud H, Chouchane A, Abderrazek E, Debbabi H, Zaouali-Ajina
M, Tabka Z, Ben-Jebria A.
Physiology and Func onal Tes ng Department 99/UR 08-67, Clinical
Laboratory of Physiology, Medical School of Sousse, Sousse, Tunisia.
OBJECTIVE: It is now well recognized that obesity is a major public health
concern, and its prevalence has tremendously increased worldwide over
the last decades, including Tunisia. As obesity is associated with
cardiovascular diseases, the purpose of this study was to investigate the
effect of obesity on forearm skin blood flow (FSBF) response to
acetylcholine (Ach), an endothelium-dependent vasodilator, in Tunisian
women over a wide range of body mass indices (BMIs).
34+/-6 years, an average height of 162+/-7 cm and an average weight of
78+/-19 kg par cipated in this inves ga on. The mean BMIs of the 60 lean,
50 overweight and 70 obese subjects were 22.1+/-0.3, 27.7+/-0.2 and
38.4+/-0.7 kg m(-2), respec vely.
Doppler flowmeter in response to local infusion of a cumulative dose of
signiﬁcantly greater in lean (1168%+/-78) than in overweight (643%+/-38)
and obese subjects (323%+/-18) (P=0.002; P<0.0001, respec vely),
suggesting a reduction of the endothelium-dependent nitric oxide (NO)
release by obesity. Our regression analysis also revealed that the maximum
FSBF response to Ach (that is, its efficacy) was inversely correlated with
BMI, waist and hip circumferences (r=-0.994, P=0.002; r=-0.2, P<0.0001,
and r=-0.321, P=0.001, respec vely).
CONCLUSION: Our data demonstrate a reduction of skin vasodilatory
reserve in obese patients and suggest a defect of both endothelial-
dependent relaxation and wall compliance associated with obesity.
Nutrition. 2010 Jan;26(1):75-81.
Dietary Intakes of Essential Nutrients among Arab and
Berber Ethnic Groups on Rural Tunisian Island.
Baroudi T, Maiz HB, Abid HK, Benammar-Elgaaied A, Alouane LT.
Laboratory of Genetics, Immunology and Human Pathologies, Faculty of
Sciences of Tunis, El Manar II University, Tunis, Tunisia. Thourbar@yahoo.fr
OBJECTIVE: The dietary intake was investigated and food sources were
identified among Tunisian ethnic groups from Jerba Island in the south of
Berbers and 47 Arabs) aged 32 to 64 y completed a 1-mo qualitative food-
frequency ques onnaire and a single 24-h dietary recall, and dietary intakes
and demographic status were observed from 2006 to 2007.
RESULTS: The prevalence of overweight and obesity was not significantly
associated with Arab men compared with Berber men. Therefore, obesity
was signiﬁcantly associated with Berber women (P<0.001). Height was
signiﬁcantly diﬀerent between Arab and Berber women (P<0.001). There
were no significant differences in energy intake between men and women.
Protein intake was not significantly different between ethnic groups. Milk
and dairy products in the Berber group were significantly different from the
Arab group. Intakes of calcium, zinc, iron, and folate were below
recommended nutrient intakes in men and women in the two ethnic
groups. Vitamin E intake was greater in Berbers than in Arabs (P<0.01).
CONCLUSION: Ethnicity was significantly associated with dietary intakes in
the two ethnic groups of Jerba Island.
Ann Endocrinol (Paris). 2009 Dec;70(6):462-7. Epub 2009 Aug 15.
Binge Eating Disorder and Obesity: Epidemiological,
Clinical and Psychopathological Aspects. A Study of an
Obese Population in Sfax (Tunisia).
[Article in French]
Ketata W, Aloulou J, Charfi N, Abid M, Amami O.
Service de psychiatrie B, CHU Hedi-Chaker, Sfax, Tunisie.
OBJECTIVES: To evaluate the prevalence of binge eating disorder (BED) in a
population of obese patients, to appreciate the impact of obesity on BED
through a comparison between the obese group and a control group, and
to assess anxiety, depression and quality of life in obese patients with BED.
PATIENTS AND METHODS: A cross-sec onal study including 60 obese
pa ents and 60 controls. BED was diagnosed using the Binge Ea ng Scale.
Quality of life was assessed by the Quality Of Life, Obesity and Dietetics
Scale, and depression and anxiety symptoms by the Hospital Anxiety and
group (40% versus 8.3%; p < 0.001; OR = 3.5). The average score of BES was
also higher (p < 0.001). Obese pa ents with BED were younger (p = 0.034).
BED was correlated with an early onset of obesity (p = 0.01; OR = 1.12),
depression (p = 0.002), anxiety (p = 0.008) and a poorer quality of life.
BED, which is correlated with a high prevalence of anxiety and depression
and with a poorer quality of life.
Clin Biochem. 2009 Nov;42(16-17):1642-7. Epub 2009 Sep 3.
Gender-Speciﬁc Eﬀect of Pro12Ala Polymorphism in
Peroxisome Proliferator-Activated Receptor Gamma-2
Gene on Obesity Risk and Leptin Levels in a Tunisian
Ben Ali S, Ben Yahia F, Sediri Y, Kallel A, Ftouhi B, Feki M, Elasmi M, Haj-
Taieb S, Souheil O, Sanhagi H, Slimane H, Jemaa R, Kaabachi N.
Research Laboratory LR99ES11, Biochemistry Department, Rabta University
Hospital, Tunis, Tunisia.
Pro12Ala (rs1801282) polymorphism of the peroxisome proliferator-
activated receptor gamma-2 (PPARgamma-2) gene on obesity or body mass
index (BMI) and plasma leptin, insulin, adiponectin and lipid levels in a
sample of the Tunisian population.
control subjects. The Pro12Ala genotype was determined by polymerase
chain reaction followed by a digestion with the restriction of endonuclease
RESULTS: In the whole population, there is no significant difference in
genotype frequencies of the Pro12Ala polymorphism between obese
patients and controls. However, separate analysis by gender revealed that
obese men (but not women) had significantly higher frequency of Pro/Ala
genotypes compared to controls (12.2% vs. 4.1%; chi(2)=6.76, p=0.009). In
comparison to Pro/Pro homozygotes, Ala-allele bearers had a significantly
higher risk of obesity [OR (95% CI)=3.26 (1.28-8.33)]. When obese subjects
were stra ﬁed according to type 2 diabetes status, the associa on with
obesity was only significant in obese non-diabe c pa ents [OR (95%
CI)=3.74 (1.43-9.74), p=0.007]. Addi onally, obese male patients carrying
the Ala-allele had signiﬁcantly higher body mass index (p=0.007) and
plasma lep n levels (p=0.023) compared to those homozygous for Pro-
allele. The signiﬁcant eﬀect of Pro12Ala polymorphism on plasma lep n
levels disappeared after adjustment for age and BMI.
CONCLUSION: The present study provides evidence that the Pro12Ala
polymorphism of the PPARgamma-2 gene is associated with obesity in non-
diabetic men from Tunisian origin.
Clin Biochem. 2009 Jul;42(10-11):1169-73. Epub 2009 Mar 28.
The G1057D Polymorphism of IRS-2 Gene Is Not
Associated with Type 2 Diabetes and Obese Pa ents
among Ethnic Groups in Tunisian Population.
Ouederni TB, Sanchez-Corona J, Flores Martinez SE, Ben Maiz H, Skhiri HA,
Abid HK, Benammar-Elgaaied A.
Laboratory of Genetics, Immunology and Human Pathologies, Faculty of
Sciences of Tunis, El Manar II University, 2092 Tunis, Tunisia.
BACKGROUND: Type 2 of diabetes is the most common metabolic disorder
and results from the interaction between genetic and environmental
factors. Insulin receptor substrate-2 (IRS-2), one of the major substrates of
the insulin receptor, has a crucial role in insulin signalling and in beta cell
development and survival. While several polymorphisms have been
identified in the IRS-2 gene, the associa on of the Gly1057Asp
polymorphism with type 2 diabetes has been studied in European and
Chinese populations, but the results have been inconsistent.
OBJECTIVES: The aim of this study was to investigate the association of
Gly1057Asp polymorphism in insulin receptor substrate-2 (IRS-2) gene
among pa ents with type 2 diabetes in well deﬁned ethnic groups from
Djerba Island in Southeastern Tunisia.
METHODS: The studied popula on (172 Arabs and 100 Berbers) includes
162 pa ents with type 2 diabetes and 110 healthy controls. BMI was
calculated for each subject. The subjects were unrelated and randomly
selected Arabs and Berbers were equally distributed between controls and
diabe cs. The G1057D polymorphism of the IRS-2 gene was genotyped
using PCR-RFLP assay.
RESULTS: This case/control study indicated that frequency of the IRS-2
Gly1057Asp polymorphism was not signiﬁcantly diﬀerent between the
healthy controls and type 2 diabe c groups, neither between healthy nor
obese subjects, in both ethnic groups. Moreover, this polymorphism is
present at a lower frequency in Djerbian than in neighbouring European
CONCLUSION: These results strongly argue against a major role of the
Gly1057Asp IRS-2 polymorphism in the pathogenesis of type 2 diabetes in
Int J Cardiol. 2009 Jun 12;135(1):130-1. Epub 2008 May 20.
Prevalence of the Metabolic Syndrome among Urban
Schoolchildren in Sousse, Tunisia.
Harrabi I, Bouaouina M, Maatoug J, Gaha R, Ghannem H.
No evidence exists regarding the prevalence of the metabolic syndrome in
adolescents in North African countries. We aimed to estimate the
prevalence of the metabolic syndrome in Tunisian adolescents. A
representa ve sample of 1569 adolescents was enrolled. The prevalence of
the metabolic syndrome was 0.4% with no sta s cal diﬀerence according
to sex (p > 0.05). Overweight/obesity was the most common component of
the metabolic syndrome (13.7%). This informa on will be used to develop
prevention programs by promoting healthy lifestyles in schools.
Clin Biochem. 2009 May;42(7-8):584-8. Epub 2008 Nov 13.
Association of G-2548A LEP Polymorphism with Plasma
Leptin Levels in Tunisian Obese Patients.
Ben Ali S, Kallel A, Ftouhi B, Sediri Y, Feki M, Slimane H, Jemaa R, Kaabachi
Biochemistry Department, Research Laboratory LR99ES11, Rabta Hospital,
OBJECTIVES: The aim of this study was to examine the association of the G-
2548A polymorphism of the human lep n gene (LEP) with body mass index
(BMI), plasma leptin, insulin, and lipid parameters in a sample of Tunisian
DESIGN AND METHODS: Two hundred and twenty nine obese patients
(BMI>or=30 kg/m(2)) were screened and compared to 251 normal weight
subjects (BMI<25 kg/m(2)). The human lep n gene promoter G-2548A
genotype was determined by polymerase chain reaction followed by a
digestion with the restriction of endonuclease CfoI.
significantly lower leptin levels than homozygous for -2548G allele (14.28+/-
9.10 ng/mL vs. 18.27+/-12 ng/mL, p<0.001 respec vely) adjusted for BMI
and gender. In obese patients but not control, subjects carrying the -2548A
allele exhibited lower lep n levels than those with GG genotype (16.96+/-
8.27 ng/mL vs. 21.37+/-11.72 ng/mL, p=0.001 respec vely) adjusted for
BMI and gender. In this group, carriership of the -2548A allele was
identified, by multiple linear regression models, as significant independent
predictor for leptin levels variability. Separate analyses by gender revealed
that only in obese women, the -2548A allele was found to be associated
with lower lep n levels independently of BMI (p=0.004).
is associated with lower leptin levels in Tunisian obese women
Rev Epidemiol Sante Publique. 2009 Apr;57(2):87-92. Epub 2009 Apr 3.
Prevalence of Conventional Cardiovascular Risk Factors in
the Great Tunis Population
Elasmi M, Feki M, Sanhaji H, Jemaa R, Haj Taeib S, Omar S, Mebazaa A, El
Ati J, Hsairi M, Kaabachi N.
Service de biochimie, hôpital La Rabta de
, 1007 Jebbari,
main cardiovascular risk factors in the population of Great Tunis.
SUBJECTS AND METHODS: This cross-sec onal study included 2483
individuals aged 35 to 70 years dwelling in the Great Tunis region, recruited
between March 2004 and June 2005. The sample was weighted using the
inverse of response rate according to governorate, district and sex.
RESULTS: Obesity and abdominal obesity were observed respec vely in 34
and 48% of subjects. The prevalence of these two factors was par cularly
elevated in females (46 and 69% respec vely). Hypertension was common
(31%), especially in women (36%). Diabetes mellitus and dyslipemia were
found in 15 and 21% of subjects, respectively, without difference according
to sex. More than half of men and 8% of women were current smokers.
dramatically high in the population of Great Tunis. These findings predict a
future expansion of cardiovascular diseases in this population. Profound
changes of lifestyle and dietary habits of Tunisians are needed to reduce
the risk of cardiovascular morbidity and mortality.
Arch Med Res. 2009 Apr;40(3):186-90. Epub 2009 Apr 5.
LEPR P.Q223R Polymorphism Inﬂuences Plasma Lep n
Levels and Body Mass Index in Tunisian Obese Patients.
Ben Ali S, Kallel A, Sediri Y, Ftouhi B, Feki M, Slimene H, Jemaa R, Kaabachi
Biochemistry Department, Research Laboratory LR99ES11, Rabta University
Hospital, Tunis, Tunisia.
BACKGROUND: The leptin receptor (LEPR) plays a crucial role in the
regulation of body weight. Several common polymorphisms have been
described in the human LEPR gene including the p.Q223R polymorphism
(rs1137101). The associa on of this polymorphism with obesity or related
metabolic phenotypes has been controversial. The aim of this study was to
inves gate the impact of the LEPR p.Q223R polymorphism on body mass
index (BMI), plasma leptin and lipid parameters in a sample of the Tunisian
METHODS: The study included 391 obese pa ents and 302 normal weight
subjects. LEPR p.Q223R genotypes were iden ﬁed by the PCR-RFLP analysis.
RESULTS: Obese patients homozygous for RR genotype showed lower leptin
levels than those with other genotypes (p = 0.005) adjusted for age, BMI
and gender. Stratified analysis by gender revealed that obese male patients
carrying the R allele showed signiﬁcantly lower BMI (p = 0.007) and lep n
levels (p = 0.037) than subjects homozygous for the Q allele. In obese
women, the LEPR p.Q223R polymorphism was found associated with lower
lep n concentra ons (p = 0.05). A er adjustment for age and BMI, the
association between the LEPR variant and plasma leptin remained
signiﬁcant only within female pa ents (p = 0.027). A general linear model
including leptin as dependant variable and age, BMI, menopausal status
and genotype as covariates revealed that the LEPR p.Q223R polymorphism
is independently associated with leptin levels in obese women (p = 0.026).
CONCLUSIONS: Our ﬁndings suggest that the LEPR p.Q223R polymorphism
influences plasma leptin levels and BMI in obese patients.
Obes Rev. 2009 Mar;10(2):145-53. Epub 2008 Nov 17.
Prevalence of Obesity and Associated Socioeconomic
Factors among Tunisian Women from Different Living
Beltaïfa L, Traissac P, El Ati J, Lefèvre P, Romdhane HB, Delpeuch F.
Institut National de Nutrition, Tunis, Tunisia.
Adult Tunisian women aged 20-59 (na onal random sample, n = 1849),
were assessed with respect to environmental and socioeconomic factors
associated with obesity (body mass index >or=30 kg m(-2)) and abdominal
obesity (waist circumference >or=88 cm). At the na onal level, prevalence
of obesity and abdominal obesity were, respec vely, 22.6% and 29.2%, but
varied markedly (both P < 0.0001) among living environments classiﬁed as
big ci es (30.2% and 36.6%), other ci es (25.9% and 32.4%), rural clustered
(19.4% and 24.8%) and rural dispersed (9.5% and 16.5%). Adjusted
prevalences of both types of obesity increased with age, parity and
economic level of the household, while educationally, the risk was greatest
in women with intermediate schooling. Differences between the four
environments were accounted for by socioeconomic factors, mostly
household wealth, except for most rural environment; socio-cultural factors
were possibly influential. Observed differences between rural areas
confirmed that finer measures of urbanization are necessary for the drivers
of obesity prevalence at the national level. Obesity was still more prevalent
in wealthy than in poor women, but given the high prevalence in all the
environments, actions are needed at the national level before highly
prevalent obesity extends into those of lower socioeconomic status and
thereby increases health inequities.
Download 5.37 Mb.
Do'stlaringiz bilan baham:
ma'muriyatiga murojaat qiling