Investigating physiological and biochemical
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Muhammad Abdul Qayyum UAF 2015 Soil Env Sciences
3.1.3.2. Ionic Tolerance
Data regarding Na + , K + and Na + /K + ratio in the leaves of linseed genotypes was subjected to cluster analysis for similar ranking as done for growth parameters. But linseed genotypes showed different degree of Na + and K + ion accumulation in leaves and hence different ranking regarding ionic parameters. However, it was noted that most of the genotypes which were ranked salt tolerant in growth parameters, also maintained the same ranking regarding ionic parameters. Only two genotypes M-319 and L×10-77 which expressed tolerance regarding growth parameters, ranked moderately tolerant in ionic parameters. On the whole, most of the linseed genotypes were ranked moderately tolerant to salinity stress while few genotypes showed sensitivity to salt stress in terms of Na + , K + and Na + /K + ratio in leaves at vegetative 61 stage of growth (Table 3.1.3). The genotypes containing very high Na + /K + ratio were considered salt sensitive while others containing fairly low Na + /K + ratio were ranked salt tolerant (Fig 3.1.2). Most of the genotypes which produced relatively higher biomass showed low Na + /K + ratio in leaves under saline conditions. Table 3.1.3. Ranking of sixty linseed genotypes for their relative salt tolerance in terms of ionic parameters (Na + , K + , Na + /K + ratio) in a cluster analysis (Ward’s minimum variance analysis) Genotypes NaCl (mM) Cluster group ranking a Sum b Genotypic ranking c Tolerance degree NO-303, E-316, 11-2, 97007, L×10-3, NM-9, 637-72 100 1 2 1 Tolerant 200 1 NM-2,ASTLIA-9, 97001, NM-10, NM-6, RL-70, 336153-538-2FR, DRM-311, L×10 -165, L×10 -10-39, 98004, 100 1 3 2 Tolerant 200 2 NM-12, NM-1, L×10 -101, M-6-A, 100 1 4 3 Moderate 200 3 L-9-20-9, 100 1 5 4 Moderate 200 4 97019, NM-14, L×10 -10, 100 2 3 2 Tolerant 200 1 2×10-7, 4506-13, NL-31, 6173-8-33, L×10-77, L×10-10-10, 166-L×10, L×10-11, 100 2 4 3 Moderate 200 2 M-319, NM-4, 6601-6, 230-27-680-4, L×10 -54, 97017, L×10 -207, 100 2 5 4 Moderate 200 3 62 E28-1, M-303, L×10-163 100 3 4 3 Moderate 200 1 NM-92-17, NM-3, E-96, L×10-29 100 3 5 4 Moderate 200 2 L×10-7-1, L×10-164, S-907, L×10-162, F-18-1, C-99-3-115 100 3 6 5 Sensitive 200 3 L×10-103, L×10-168 100 3 7 6 Sensitive 200 4 LA-10-127-1 100 4 6 5 Sensitive 200 2 0173-13-10, 6148-51-12 100 4 7 6 Sensitive 200 3 GP98-3-45 100 4 8 7 Sensitive 200 4 a Cluster groups were obtained from Ward’s minimum variance cluster analysis on the means of the salt tolerance indices in seedling ionic parameters (see Material and Methods). Genotypes were divided into four cluster groups at 100 mM NaCl and four cluster groups at 200 mM NaCl. The cluster group rankings were obtained from cluster means (data not shown) in the order from the highest to the lowest cluster means. b Sums were obtained from the cluster group ranking by adding the ranking numbers at the two salt levels in each genotype. c Genotypes were finally ranked based on the sums with the smallest sum being the most relatively tolerant |
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