Quality control methods for medicinal plant materials
20. Culture media and strains of microorganisms
Culture media
The following media are satisfactory, but other media may be used if they have
similar nutritive and selective properties for the microorganisms to be tested.
Baird-Parker agar
Procedure. Dissolve 10.0 g of
pancreatic digest of casein R, 5.0 g of beef extract R,
1.0g of water-soluble
yeast extract R, 5.0
g of lithium chloride R, 20.0 g of agar R,
12.0 g of glycine R and 10.0 g of sodium pyruvate R in sufficient water to
produce 950 ml. Heat to boiling for 1 minute, shaking
frequently and adjust the
pH to 6.6-7.0 using sodium hydroxide (0.5 mol/l) VS. Sterilize in an autoclave at
121°C for 15 minutes, cool to 45-50°C and add 10 ml of a sterile 0.01 g/ml
solution of potassium tellurite R and 50 ml of egg-yolk emulsion.
Brilliant green agar
Procedure. Dissolve 10.0 g of dried peptone R (meat and casein), 3.0 g of water-
soluble yeast extract R, 5.0 g of sodium chloride R, 10.0 g of lactose R, 10.0 g of
sucrose R, 20.0
g of agar R, 0.08 g of phenol red R and 12.5 mg of brilliant green
R in sufficient water to produce 1000 ml. Heat to boiling for 1 minute. Using
sodium hydroxide (0.05 mol/l) VS adjust the pH to 6.7-7.1.
Immediately before
use, sterilize in an autoclave at 121°C for 15 minutes, cool to 50°C and pour into
Petri dishes.
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