Quality control methods for

MacConkey broth 
Procedure. Dissolve 20.0 g of pancreatic digest of gelatin R, 10.0 g of lactose R, 5.0 
g of dehydrated ox bile R and 10 mg of bromocresol purple R in sufficient water 
to produce 1000 ml. Using sodium hydroxide (0.05 mol/l) VS adjust the pH to 
7.1-7.5. Sterilize in an autoclave at 121°C for 15 minutes. 
Sabouraud glucose agar with antibiotics 
Procedure. Dissolve 10.0g of dried peptone R (meat and casein), 40.0g of glucose 
hydrate R and 15.0g of agar R in sufficient water to produce 1000ml. Using acetic 
acid (~60 g/l) TS adjust the pH to 5.4-5.8. Sterilize in an autoclave at 121°C for 15 
minutes. Immediately before use, add sterile solutions of 0.10g of 
benzylpenicillin sodium R and 0.10g of tetracycline R per litre of medium or 
alternatively, before sterilization, add 0.050g of chloramphenicol R per litre of 
medium. 
Soybean-casein digest medium 
Procedure. Dissolve 17.0g of pancreatic digest of casein R, 3.0g of papaic digest of 
soybean meal R, 5.0g of sodium chloride R, 2.5g of dipotassium hydrogen 
phosphate R and 2.5g of glucose hydrate R in sufficient water to produce 1000 
ml. Using sodium hydroxide (0.05 mol/l) VS adjust the pH to 7.1-7.5. Sterilize in 
an autoclave at 121°C for 15 minutes. 

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Quality control methods for medicinal plant materials 
Tetrathionate bile brilliant green broth 
Procedure. Dissolve 8.6g of dried peptone R, 8.0 g of dehydrated ox bile R, 6.4g of 
sodium chloride R, 20.0g of calcium carbonate RI, 20.0g of potassium 
tetrathionate R and 0.070g of brilliant green R in sufficient water to produce 1000 
ml. Using sodium hydroxide (0.05 mol/l) VS adjust the pH to 6.8-7.2. Heat just 
to boiling; do not reheat. 
Triple sugar iron agar 
Procedure. Dissolve 3.0 g of beef extract R, 3.0 g of water-soluble yeast extract, 
20.0 g of dried peptone R (casein and beef), 5.0 g of sodium chloride R, 10.0 g of 
lactose R, 10,0 g of sucrose R, 1.0 g of glucose hydrate R, 0.3g of brown 
ammonium iron(III) citrate R, 0.3 g of sodium thiosulfate R, 25 mg of phenol red 
R and 12.0 g of agar R in sufficient water to produce 1000 ml. Heat to boiling for 
1 minute with shaking. Using sodium hydroxide (0.05 mol/l) VS adjust the pH 
to 7.2-7.6. Distribute in tubes and sterilize in an autoclave at 121°C for 15 
minutes. Allow to set in an inclined position covered with a butt.