Сборник V международной

THE STUDY OF THE VASORELAXANT EFFECTS OF MATRICARIA 
CHAMOMILLA EXTRACT ON L - TYPE CA2+ -CHANNELS
Omonturdiev S.Z., Inomjanov D.R., Gayibov U.G., Abdullaev A.A.,
Aripov T.F., Usmanov B.P.
Institute o f Bioorganic Chemistry named after A S Sodiqov o f the Academy o f Sciences o f the Republic
o f Uzbekistan,
Institute o f Biophysics and Biochemistry at the National University o f Uzbekistan,
Namangan State University 
e-mail: [e-mail]
Importance. L-type Ca2+-channels are Ca2+transporters. Their activation or opening occurs when 
the membrane potential is -50 mM, in which case they remain in a conductive state for a long time. Their 
naming is also derived from this (long-lasting). Ca2+ ions entering through these channels not only provide 
the action potential, but also participate in the activation of smooth muscle cells’.
Aim. The purpose o f this study was to determine the mechanism o f relaxant effect on the functional 
activity of the aortic blood vessels in rats under Matricaria chamomilla extracts which isolated from an 
endemic one.
Materials and methods. The experiments were performed under isometric conditions, white rats 
(200-250 g) in aortic vascular preparation. Experimental medium (mM): NaCl - 120.4; KCl - 5; NaHCO3 
- 15.5; NaH2PO4 - 1,2; MgCl2 - 1,2; CaCl2 - 2.5; С Д 20 6 - 11.5 (rN = 7.4) (Russia).
Results. It is known that the contractile activity of the aortic preparation caused by KCl (50 mM) is 
determined by the activation of potential-dependent Ca2+-channels located in the plasmalemma o f smooth 
muscle cells. At the same time, the value o f the membrane potential changes with increasing concentration 
of K+ ions in the environment, which, in turn, leads to membrane depolarization and activation of poten­
tial-dependent Ca2+-channels and contraction due to increased concentration o f Ca2+ions in the cytoplasm of 
cells. In this regard, the effect o f Matricaria chamomilla extract on the contraction o f rat aortic preparation 
induced by KCl (50 mM) was studied in experiments. It was found that this extract has a strong relaxant 
effect in the case of concentration range o f 10 - 80 pg / ml. Also, the amplitude o f aortic preparation con­
traction activity at a concentration o f 10 mcg / ml relative to the control 81,8 ± 2.9%.