Сборник V международной

Conclusion. The results obtained indicate that, the relaxant effect o f Matricaria chamomilla extract 
may be due to blockade o f potential-dependent Ca2+-channels located in the sarcolemma o f smooth muscle 
cells and a decrease in the influx o f Ca2+ions into their cytoplasm.
DEVELOPMENT OF A METHOD FOR THE DETERMINATION OF 
HYALURONIC ACID IN MEDICINAL PRODUCTS
Khamidov Sh.A., Smanova Z.A.
Mirzo Ulugbek National University o f Uzbekistan 
e-mail: [e-mail]; тел.[phone]
Relevance. Today, in the world, chemical aspects in the pharmaceutical industry are of paramount 
importance, both in the production of medicines and in the process of quality control of intermediate and 
finished products, as well as during the input control of raw materials and materials. Therefore, there is 
an increasing need to find alternative, the most advanced, new physico-chemical methods for determining 
substances in medicines to assess their quality. The development o f the simplest, most accessible, express, 
reliable, universal, budgetary methods of analysis is of great practical importance and is relevant.
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Purpose: development and validation of methods for the analysis o f hyaluronic acid in medicinal 
products.
Materials and methods. In the course o f the work, spectrophotometric and chromatographic 
(HPLC) methods of analysis were used. In the process o f sample preparation, the derivatization method was 
used for analysis. In accordance with the recommendations o f ICH Topic Q2 (R1) Validation o f Analytical 
Procedures: Text and Methodology, validation parameters for the analytical procedure such as accuracy, 
correctness, specificity, linearity and reproducibility were used.
The results obtained. Optimal conditions for the possibility of spectrophotometric determination of 
hyaluronic acid have been selected, while the dependence of the data obtained on various factors (reagent 
concentration, immobilization time, pH of the medium, and others) has been established, at the same time, 
some physico-chemical characteristics of the obtained complexes have been determined and calculated. All 
measured parameters: specificity, linearity, convergence, correctness meet the acceptance criteria. After the 
validation o f the determination of the quantitative content of active substances in medicines, a comparative 
analysis of the methods previously described in the pharmacopoeia with the method developed by us was 
carried out. As a result o f our comparative analysis, it was revealed that the data obtained using the spectro- 
photometric method correspond to all validation parameters and are the most reliable, compared with the 
data obtained by the microbiological method.
Conclusions. For the first time, an affordable and easy-to-perform spectrophotometry method has 
been developed and optimized to confirm the authenticity and determine the quantitative content of hyal­
uronic acid in the composition o f medicines.