Сборник V международной

candidate genes is human canceronida, including the TP53 gene known as the “tumor suppressor gene”, 
“genome keeper” or “cell keeper o f growth and division”, which is located on - 17p13.1 chromosome. 
The question of the study of the formation and development of this disease is currently a topical prob­
lem of modern medicine. Various endogen and exogen changes are important in improving breast cancer 
treatment. Also, the study of biochemical analysis o f peripheral blood is becoming a reliable criterion for 
assessing human health.
The purpose of the work. 
To analyze the association o f biochemical and TP53 gene rs17884159 
polymorphism in the development of breast cancer.
Materials and methods. 
For the study, 54 women were diagnosed with breast cancer. The results 
of biochemical analysis BA88A (Mindray, Kitay) in the semi-automatic biochemical analyzer were deter­
mined using the test-kits prepared for biochemical analysis of HUMAN (Human GMBH; Germany) from 
the peripheral blood o f patients. Quantitative indicators of the general blood test were checked in the he­
matological analyzer Mindray (China) or Sysmex. The studied samples were separated from the peripheral 
blood by DNA using “Amplipraym ribo-prep” (OOO “Nekst Bio”, Russia) and “Diatom TM DNA Prep 
100” (laboratory izogen, Russia). The amount and quality of DNA was checked using a NanoDrop 2000 
(Thermo Fisher Scientific, USA) spectrophotometer. The C/T polymorphism o f the TP53 gene was tested 
using the Sintol genetic test kit according to the the manufacturer’s instructions. The polymer chain reaction 
was performed in a Rotor-Gene Q amplifier Gene Q (QIAGEN Hilden, Germany). Statistical analysis of 
the obtained results were carried out using statistical computer programs “WinPEPI 2016, Version 11.65”