SingHealth Core Platforms Equipment (Updated: 31 March 2016) Equipment Brand / Model Capabilites Utilization of equipment Location Contact details Website
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- Equipment Brand / Model Capabilities Utilization of Equipment Location Contact details
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- SingHealth Core Platforms Equipment (Updated: 31 March 2016) Equipment Brand / Model Capabilities
- Equipment Brand / Model Capabilities Utilization of equipment Location Contact details
- SingHealth Translational Immunology and Inflammation Centre (STIIC)
- SingHealth Flow Cytometry Core
- SingHealth Tissue Repository / Advanced Molecular Pathology Lab
- Utilization of Equipment Location Contact details Website
- SingHealth Advanced Bioimaging Core
SingHealth Core Platforms Equipment (Updated: 31 March 2016) Equipment Brand / Model Capabilites Utilization of equipment Location Contact details Website NanoPET/MRI Mediso / NanoScan PET subsystem - High resolution imaging of functional processes in small animals (mice and rats) with the use of radiotracers. MRI subsystem - Imaging of soft tissue morphology. Service Provision NanoCT/SPECT Mediso / NanoScan CT subsystem - High resolution imaging of anatomical structures in small animals (mice and rats) using X-Ray SPECT subsystem - High resolution spatial imaging of anatomical structures in small animals (mice and rats) using gamma rays. Service Provision C-Arm Fluoroscope Siemens / Avantis Real time x-ray imaging of large animals. Service Provision SEMC Academia Basement 1, Main OT
IVIS Spectrum Caliper Lifesciences In-vivo optical imaging for small animals. Trained researchers allowed to use the equipment independently OptoAcoustic scanner iTheraMedical View / MSOT Optical imaging using photoacoustic effect to obtain images of structures in turbid environments. Service Provision Rodent Ultrasound Machine S-Sharp Prospect Real time and non-invasive image acquisition capabilities for in-vivo small animal studies. For visualisation of molecular events or quantification of tumour size. Service Provision SEMC Academia Basement 1, Microsurgery Room Laparascopic System Karl Storz For performing minimally invasive surgery (MIS) through small incisions on large animals. Trained researchers allowed to use the equipment independently SEMC Academia Basement 1, Large Animal Procedure 1 Large Animal Ultrasound Machine GE Logiq S7 For non-invasive image acquisition of large animals like pigs. Service Provision SEMC Academia Basement 1, Main OT
NHP PET-CT Mediso MultiScan LFER PET-CT For high resolution of imaging of lying and awake macaques. Service Provision SEMC Academia Basement 1, Fluoroscopy Lab 7.0 Tesla MicroMRI for Rodents KIV purchase 7.0 Tesla strength magnet for imaging of rodents. Service Provision SEMC Academia Basement 1, MicroMRI, availability KIV. Equipment Brand / Model Capabilities Utilization of Equipment Location Contact details Website LC-MS/MS
Agilent LC with ABSCIEX 5500 QTRAP system HPLC Shimadzu/ Prominence LC Pyrosequencer Qiagen/PyroMark Q24 Quantitative analysis of genetic or epigenetic DNA modifications
SEMC Academia Basement 1, Rodent Bioimaging Dr. Bryan Ogden email: bryan.ogden@singhealth.com.sg Enquiry email: shs.semc@singhealth.com.sg http://research.singhealth.co m.sg/Pages/SingHealthExper imentalMedicineCentre.aspx SEMC Academia Basement 1, Optical Imaging SingHealth Clinical Pharmacology Core Separation, identification and quantification of drug compounds and metabolites All Service provision (Not Available for Researchers' Independent use) 20 College Road, The Academia, Discovery Tower, Level 8, Clinical Pharmacology Core Prof Balram Chowbay Phone: 65767096 Enquiry email: clin.pharm@singhealth.com.sg http://research.singhealth.co m.sg/Pages/clinical- pharmacology.aspx
SingHealth Core Platforms Equipment (Updated: 31 March 2016) Equipment Brand / Model Capabilities Utilization of equipment Location Contact details Website CyTOF 2
DVS Mass Cytometry FACS ARIA III SORP (4 laser) BD Flow Cytometry and Cell sorting Nanostring Nanostring Pathway analysis, Copy Number Variation, gene expression analysis QX100 Biorad
Digital droplet PCR LC480
Roche Real Time PCR analysis Enspire Multimode plate reader Perkin Elmer ELISA reader
LSRFortessa SORP BD / 647800L6 5-laser flow cytometer, detects up to 18 colors. For cell analysis only For trained researchers to use independently FACSAria III BD / 648282-23-010100-X- X-Z
4-laser flow cytometer with cell sorting ability. Detects up to 16 colors. Service provision BD Canto II BD/ 338960 2-laser cytometer, for cell analysis only. Detects up to 6 colors. For trained researchers to use independently BD LSRII (SGH Asset) BD / 33300476 4-laser cytometer, for cell analysis only. Detects up to 13 colors. For trained researchers to use independently autoMACS Pro Cell Separator (rented) Miltenyi Biotec / 130-092- 545
Automated magnetic cell separator Capable of performing automated cell labelling For trained researchers to use independently Equipment Brand / Model Capabilities Utilization of equipment Location Contact details Website Ion Torrent PGM Life Technologies Next-generation sequencing Miseq Next-generation Sequencer Illumina
Next-generation sequencing Equipment Brand / Model Capabilities Utilization of equipment Location Contact details Website Leica Bond III Leica Immunohistochemistry and in-situ hybridization Zeiss Microscope with Imaging System Zeiss Fluoroscence Imaging 20 College Road, The Academia, Discovery Tower, Level 10, Tissue Repository Magdalene Koh Phone:65767116 http://research.singhealth.co m.sg/pages/singhealthtissuer epository.aspx SingHealth Translational Immunology and Inflammation Centre (STIIC) Available for use by trained researchers following a collaborative agreement only. 20 College Road, The Academia, Discovery Tower, Level 8, STIIC Lakshmi Ramakrishna Phone: 65767154 http://research.singhealth.co m.sg/pages/stiic.aspx
20 College Road, The Academia, Discovery Tower, Level 10, Flow Cytometry Core Charlene Foong Phone: 6576 7178 Enquiry Email: flow@singhealth.com.sg http://research.singhealth.co m.sg/Pages/flow- cytometry.aspx POLARIS@SingHealth All Service provision (Not Available for Researchers' Independent use) 20 College Road, The Academia, Discovery Tower, Level 10, POLARIS@SingHealth Ms Tan Gek San / A/Prof Lim Kiat Hon, Tony Enquiry Email: polaris@singhealth.com.sg http://research.singhealth.co m.sg/Pages/polaris.aspx SingHealth Tissue Repository / Advanced Molecular Pathology Lab All Service provision (Not Available for Researchers' Independent use)
SingHealth Core Platforms Equipment (Updated: 31 March 2016) Equipment Brand / Model Capabilities Utilization of Equipment Location Contact details Website Zeiss Elyra super resolution microscope Zeiss ELYRA PS.1 SIM (Structured illumination microscopy) and PALM (photo-activation localisation microscopy) configurable for d-STORM, TIRF, widefield Software: Zen 2012 Laser lines: 405nm, 488nm, 561nm, 642nm Objectives: 63x oil, 100x oil Definite focus system to keep sample in focus Resolution: SIM xy: ~120nm, z: ~250nm PALM xy: 10-30nm, z: ~50nm Nikon STORM super resolution microscope Nikon N-STORM with Andor CSU-W1 spinning disk
STORM (stochastic optical reconstruction microscopy) and spinning disk confocal configurable for d-STORM, TIRF, widefield, brightfield Software: NIS Elements Laser lines: 405/445nm, 488/515nm, 561nm, 640nm Objectives: 10x, 20x, 40x oil, 60x oil, 100x oil Perfect focus system (PFS) Stage top incubator, temperature and humidity controlled Resolution: STORM xy: 10-30nm, z: 50-70nm Leica STED super resolution microscope Leica TCS SP8 STED 3X STED (stimulated emission depletion) and scanning confocal Software: LAS X Laser lines: 405nm, 470-670nm (tunable up to 8 colors) Objectives: 20x, 40x oil, 100x oil Resolution: STED xy: 50-70nm, z: 70-130nm High Content Screening Microscope Molecular Devices ImageXpress Micro High content imaging and analysis for fluorescent plates Software: MetaXpress Light source: Xenon lamp Objectives (all ELWD): 10x, 20x, 40x, 60x Fully enclosed, temperature and humidity controlled Laser capture microdissection microscope Leica LMD 7 UV laser cutting/isolation of cells or tissues for further downstream analysis of DNA, RNA etc Software: Laser micro-dissection V8.0 Light source: LED and mercury lamp Objectives: 6.3x, 10x, 20x, 40x, 63x, 150x Dissection Microscope Leica S8 APO Software: LAS Light source: LED Magnification: adjustable to 8x Brightfield Microscope Zeiss Axio Lab A1 Software: Zen Light source: Halogen lamp Objectives: 5x, 10x, 20x, 40x, 100x oil Phase contrast microscope Nikon Eclipse TS100 For cell culture observation Objectives: 4x, 10x, 20x, 40x Glass Knife Maker Leica EM KMR3 To prepare glass knives for cutting semi- and ultrathin sections on the ultra-microtome Sputter coater Leica EM ACE200 Providing homogenous and conductive metal coatings for SEM and TEM samples Critical Point Dryer Leica EM CPD300 Drying machine for biological EM specimens Staining machine with radioactive shield Leica EM AC20 Automatic contrasting system for ultrathin tissue sections Ultra microtome Leica ULTRACUT EM UC7 Sectioning machine for preparing EM semi- and ultrathin sections Transmission Electron Microscope Jeol JEM 2100 (shared with SGH Pathology) Software: Gatan Digital Micrograph Voltage: 80-200 keV Single and quartet sample holder Piezo goniometer stage Point resolution: 0.23nm 20 College Road, The Academia, Level 1, TEM Room For trained researchers to use independently, service provision also available. 20 College Road, The Academia, Discovery Tower, Level 10, Advanced Bioimaging Core Enquiry Email: bioimaging@singhealth.com.sg http://research.singhealth.co m.sg/Pages/advanced- bioimaging.aspx SingHealth Advanced Bioimaging Core Download 47.2 Kb. Do'stlaringiz bilan baham: |
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