Supramolecular complex of monoammonium salt of glycirrizinic acid with norsulfazol

participation in the complexation of the carboxyl and 
hydroxyl groups of MASGA, as well as the primary 
amino group and thiazole nitrogen atom.
H
2
N
S
O
O
N
-
N
S
Na
+
The results of quantitative and qualitative compo-
sition of complex compounds are shown in (table 2).

---

Section 11. Chemistry
80
Table 2. – Qualitative and quantitative determination of the composition of 
the complex compound MASGA: Norsulfazole-Na method by HPLC
Theoretically%
Practically%
Retention time min.
MASGA
Norsulfazol-Na
MASGA
Norsulfazol-Na
91.7
8.3
90.8
8.2
7.509
From the data given in (table No. 2) it can be 
seen that the results obtained coincide with the theo-
retically calculated data, which were confirmed by 
the results of IR and UV spectroscopy.
Experimental part.
For experiments, MASGA of 92% purity 
(by HPLC) obtained by the known method [8] and 
the substance of norsulfazole sodium (2- (p-amino-
benzenesulfamido) thiazole sodium) were used. IR 
spectra were recorded on an IRTracer-100 IR Fourier 
spectrometer (Shimadzu, Japan). UV spectra were 
recorded on a Shimadzu-1280 spectrophotometer 
in quartz cuvettes (
l = 10 mm); aqueous solutions 
of MASGA and norsulfazole were used to prepare 
solutions of the isomolar series (pH 7.2; c = 10
–4
M 
phosphate buffer). HPLC was performed on an Agi-
lent 1200 autosampler and gradient pump.
IR spectrum of HC(ν, cm
–1
): 3228(OH, NH), 
2927(CH), 1714(C = O), 1656(C
11
= O; C = C), 
1593(COO
–
), 1039(COC).
IR spectrum of norsulfazole (ν, cm
–1
): 3184 
(NH), 1440(C = N), 1238(NH), 1122(CHAr), 
677(C-S).