1BC1 backcross hybrids obtained from crossings of monosomic and monotelodisome cotton lines

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Cytogenetic analysis of aneuploid F₁BC₁ backcross hybrids obtained from crossings of monosomic and monotelodisome cotton lines (G.hirsutum L. × G.barbadense L.) × G.hirsutum L.
M.F. Sanamyan¹, Sh.U. Bobokhujayev¹, O.G. Silkova²
1. National University of Uzbekistan named after Mirzo Ulugbek, Tashkent, Uzbekistan
2. Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russian Federation
*Corresponding author email: [e-mail]
E-mail addresses of co-authors: [e-mail], [e-mail]
Annotation. The difficulties of transferring the genes responsible for improving fiber quality from the G.barbadense L. species to the G.hirsutum L. genome using interspecific hybridization are shown. Previously, the influence of certain chromosomes of the species G.barbadense L. on fiber elongation, fiber yield, microneur and other properties in chromosome-substituted lines of the species G.hirsutum L. was revealed. The aim of the article is the cytogenetic analysis ofF₁BC₁ hybrid plants using the analysis of chromosome, spores and pollen fertility.Analysis of chromosome pairing in F₁BC₁ hybrid backcross monosomics in 15 variants of crossings with substitution of chromosomes 2, 4, 6, 7, 12 of the At-subgenome and 17, 18, 21, and 22 of the Dt-subgenome revealed normal chromosome pairing, with the exception of incomplete chromosome pairing in one combination (F₁BC₁(Mo75xF₁104₂)) and the presence of a quadrivalent in another (F₁BC₁(Mo17xF₁685₇)). as well as a high meiotic index and a small number of tetrads with micronuclei, which indicated the regularity of meiotic division. However, a decrease in pollen fertility in some interspecies aneuploid F₁BC₁ hybrids indicated a latent structural variability that was not recorded at the metaphase I stage of meiosis.
The article 10 Figures, 4 Tables, 21 Referenced
Key world: cotton, G. hirsutum L. G. barbadense L., backcross hybrids, substitutions, chromosome pairing, sporades, pollen fertility.