Bank of Krok testing to module N1

A Direct and indirect immunofluorescence test
B Direct and indirect fluorescence immunoassay
C Hemagglutination inhibition assay
D Radioimmunoassay
E -

b-12 Material taken from a patient with provisional diagnosis "influenza" was referred to a laboratory. For virological examination the hemadsorption reaction was applied. This reaction can be applied for detection of the following viruses:

A Viruses containing hemagglutinins
B All the simple viruses
C All the complex viruses
D DNA-genomic viruses
E Any viruses

178. Influenza virus contains internal antigens — nucleoprotein (NP), polymerase (PI, P2, P3), matrix protein (M), and surface antigens — hemagglutinin (H) and neuraminidase (N). Which of them play the basic role in the creation of the immunity to influenza infection?

A.*Hemagglutinin and neuraminidase.
B.Nucleoprotein antigens.
C.Matrix protein.
D.Polymerase proteins.
E.Neuraminidase and nucleoprotein.

183. A human corpse is sent to a department of morbid anatomy from an infectious hospital. The preliminary diagnosis is influenza. What researches are the most rational for confirmation of this diagnosis?

A.Antibodies detecting during complement fixation test.
B.Electron microscopic detecting of viruses.
C.Detecting intracellular inclusions using light microscopy.
D.Detecting high antibody titers during hemagglutination inhibition test.
E. *Isolation and serologic identification of virus.

184. Infecting of chicken embryos is the basic method of influenza virus isolation. Before introducing the researched material (nasopharyngial swab) into a chicken embryo a sample should be preliminary decontaminated with:

A.Formalin.
B.Alcohol.
C.Eagle's solution.
D.*Streptomycin and penicillin.
E.Ether.

*05*06* 43. There is a flu epidemic in a city. Which preparation could be recommended for the nonspecific prevention of the disease?

A.*Remantadin.
B. Influenza vaccine.
C.Benzylpenicillin sodium salt.
D. Antigrippal immunoglobulin.
E. Antigrippal serum.

194. At a neonatal unit the children's morbidity with respiratory virus infections caused by different groups of viruses has sharply increased. To prevent the spread of infection it was recommended to prescribe human leukocytic interferon. How should it be introduced?

A.Via inhalation.
B.Subcutaneously.
C.*Through nasal meatuses.
D.Orally.
E.Intramuscularly.

27. A doctor in charge of immunization program makes the list of preparations to prevent a season influenza epidemic. Which one of the following is the least allergenic and the most likely to develop active specific immunity?

A.*Subunit vaccine.
B. Live vaccine.
C. Killed vaccine.
D.Donor γ-globulin.
E.α-interferon.

29. For prevention of the seasonal high rate of influenza in educational institutions doctors decided to carry out immunization of children and teenagers. Which preparation should be used for this purpose?

A. Interferon.
D.Oxolin.
B. Normal human immunoglobulin.
C. Remantadin.
E. Vaccine

*A chicken flu virus may cause mass infections among people in all countries of world. In this case it is talking about:

Epidemic
*Pandemic
Epizootic
Superinfection
Reinfection

Parainfluenza virus

* A child complaints of sudden dry (“barking”) cough, rhinitis and increased temperature. A doctor, which has been called to patient, suspects parainfluenza infection. Which result of laboratory assay may confirm diagnosis of this patient?
Hemadsorbing agent revealing in cell culture inoculated with nasopharyngeal washing
*Antibody to parainfluenza virus detection in serum
Mouse death after infection with nasopharyngeal washing
Revealing of specific virions at electron microscopy
Formation of plaques under agar covering after infection of cell culture with nasopharyngeal washing

Measles
* There were some cases of infection among children 2 weeks ago. Based onto clinical appearance and epidemiological history a doctor suspected outbreak of measles. Which variant of laboratory assay can confirm such suspicion?

*Rhinocytoscopy
Immunofluorescence test
Serology test
Chicken embryo infection
Allergy test

Mumps virus

180. A 6-year-old boy has moderate rise of the body temperature and enlarged parotid glands. From the patient's saliva a virus was isolated. This virus can replicate in chicken embryos and tissue cultures, has hemagglutination properties, and causes symplasts formation in cells culture. Which organ is the most likely to be affected with the infection caused by this virus?
A.*Sexual glands.
B.Liver.
C.Lungs.
D.Pharyngeal tonsils.
E.Brain.

Respiratory-syncitial virus

193. During the microscopy of the cellular monolayer infected by the virus-containing material from a patient a laboratory doctor has diagnosed a respiratory syncytial virus infection. What changes are caused by this virus in cell culture?
A.*Symplasts formation.
D.Appearance of Negri bodies.
B.Cluster-shaped destruction of mo­nolayer.
C.Complete destruction of cellular monolayer.
E.Cells proliferation

*After examination of a child at second day of illness a doctor suspected respiratory-syncitial infection. Which variant of laboratory assay may confirm diagnosis during first days of hospitalization?

*Detection of RS-antigens in nasopharyngeal samples
Serology (4-fold rising of antibody titer)
Ig A detection in nasopharyngeal samples
Ig M detection in nasopharyngeal samples
High antibody titers to RS-antigens in patient serum

Rubella virus

* b-12 A pregnant woman was examined for some infections during registration in maternity hospital. IgM antibody to rubella virus was detected into the serum. What does it indicate?
*Primary infection
Chronic process
A woman is healthy
Exacerbation of chronic process
Repeated infection with rubella virus

*A pregnant woman (first trimester of pregnancy), which had contact with rubella case, visited a doctor. Which recommendation may be given by doctor?

Vitamines A,E
Immunoglobulins
Antibiotics
Vitamines
*Induced abortion

Other respiratory viruses

* A patient has headache, general weakness, cough, and fever. Clinical diagnosis ARVI is made. Which from listed viruses can be cause of this infection?
The flu virus
Adenovirus
Respiratory-syncitial virus
Coronavirus
*Any of them

Hepatitis B virus

175. High-sensitivity methods are used for checking the presence of virus hepatitis B antigens in donors' blood. Which test should be used?
A. *Solid-phase enzyme immunoassay. (ELISA)
D. Complement fixation.
B. Immunoelectrophoresis.
C. Indirect hemagglutination.
E. Indirect immunofluorescence.

196. HBs-antigen has been revealed during enzyme immunoassay in serum. What infection can be diagnosed by this antigen detecting?

A. Syphilis.
B.Virus hepatitis A.
C.AIDS.
D. Tuberculosis.
E.*Virus hepatitis B.

*At donor blood assay one from the markers of hepatitis B infection was revealed. What marker detection does not allow to use this blood for hemotransfusion?

*HBs-antigen
HBc-antigen
HAV
Hbe-antibody
Hbe-antigen

b-12 The donor who for a long time didn't donate the blood was investigated with IFA method. Anti-HBs antibodies were revealed. What does positive result of IFA in this case mean?