Bank of Krok testing to module N1

A Study antigenic properties of the causative agent
B To determine sensitivity to antibiotics
C To study sensitivity to bacteriophages
D To study biochemical properties of the causative agent
E To study virulence of the causative agent

*A child with complaints onto general weakness, bloody diarrhea and pain in kidney region has been hospitalized. Body temperature is normal. There are no leucocytes at coproscopy. Indicate, which microorganism could cause such symptoms of disease?

*Enteroinvasive E.coli
Enterohemorrhagic E.coli
Enterotoxic E.coli
Enteropathogenic E.coli
Enteroadhesive E.coli

*A pure culture of E.coli has been isolated from feces of ill person. What is from given later does not correspond to characteristics of this species? E.coli is:

*Toxigenic in case of lysogenization
Representative of large intestine microbiota
Causative agent of purulent infection
Causative agent of cholera-like disoders
Active component of eubiotics

Salmonella typhi

28. After inoculation of feces specimen from a patient with typhoid fever onto Endo medium colonies of different size and color — big red and medium colorless - have grown. Name the functional type of this medium.

A. Selective.
B. Enriched.
C. Special.
D. *Differential-diagnostic.
E. Universal (general purpose).

104. From the blood of a patient there was obtained the culture of typhoid fever pathogen. What cultural properties are characteristic of this pathogen?

A. Hemolysis on blood agar.
B. Formation of red colonies with metallic luster on Endo medium.
C. Formation of colorless colonies on bismuth-sulfite agar medium.
D. *Formation of colorless colonies on Endo medium.
E. Formation of a gentle pellicle on alkaline peptone water.

166. Feces of a restaurant cook without any clinical symptoms of disease are examined with the help of bacteriological methods. On bismuth-sulfite agar little black colonies with metallic sheen have grown. What microorganisms can it be?

A. *Salmonellae.
B. Shigellae.
C. Escherichiae.
D. Staphylococci.
E. Streptococci.

40. A diagnosticum (processed with tannin sheep erythrocytes adsorbed by Vi-antigen of Salmonella typhi) has been used for serologic diagnostics of typhoid carrying. Choose the reaction, in which this diagnosticum will be used.

A. Precipitation.
Hemagglutination
*Indirect (passive) hemagglutination
Complement fixation test.
C. Hemagglutination inhibition reaction.

*07* b-12 In passive hemagglutination test made with erythrocyte thyphoid Vi-diagnosticum antibody in serum dilution 1:80 has been revealed that is higher of diagnostic titer. Such result indicates next:

1. 
   *possible carrying of enteric fever pathogen
   
2. 
   Patient recovery after enteric fever
   
3. 
   Recurrence of enteric fever
   
4. 
   Acute infection of enteric fever
   
5. 
   Incubation period of enteric fever
   

*05* 174. Inspection of school dining-room staff for typhoid carrying has been carried out. There were revealed antibodies to Vi-antigen in the blood serum of the cook. Which test has been used?

A. Complement fixation test.
B. Widal's test.
C. *Indirect hemagglutination test
D. Enzyme immunoassay.
E. Immunofluorescence test.

*02* 82. A patient with typhoid fever suspected was administered blood culture. It is rational because during the first week of enteric fever the following phenomenon is observed:

A. Toxinemia.
B. *Bacteremia.
C. Septicemia.
D. Septicopyemia.
E. Viremia.

* A patient with previous diagnosis “enteric fever” was hospitalized in a hospital. Which nutrient medium from given later may be used for blood culture isolation?

MPB
*Bile broth
Yolk-salt agar
Blood agar
Lowenstein-Jensen agar

121. b-12 A patient (has been feeling ill for 3 days) was hospitalized to an infectious clinic with typhoid fever preliminary diagnosed. What method should be used to confirm the diagnosis?

*Hemoculture. (blood culture)
Biliculture.
Coproculture.
Urine culture.
Roseola culture.

*00*01* 150. A patient with complaints of a three days' fever, general weakness, insomnia, deterioration of appetite referred to a doctor. The doctor has suspected typhoid fever. What method of laboratory diagnostics is the most rational for confirmation of the diagnosis?

A. Myeloculture.
B. Coproculture.
C. Urine culture.
D.Spinal fluid culture.
E. *Hemoculture (blood culture).

91. Pure culture was isolated from a patient with typhoid fever suspected. The culture was identified as S. typhi by morphologic, cultural, and biochemical characteristics. What investigation should be used for final identification of the causative agent?

A. Serodiagnosis.
B. *Seroidentification.
C. Allergic diagnostics.
D. Antibiotic-sensitivity test.
E. Phage typing.

101. Widal's test is used for the serologic diagnostics of typhoid fever. What mechanism of antigens and antibodies interaction underlies it?

A. Hemolysis.
B. Precipitation.
C. Bacteriolysis.
D. *Agglutination.
E. Bacteria immobilization.

142. Blood of a patient with typhoid fever has been sent to a laboratory for detecting antibodies. Which serological reaction should be used?

A. Hemagglutination inhibition.
B. Precipitation.
C. Complement binding.
D. Passive hemagglutination.
E. *Agglutination.

*A serum of patient with suspicious enteric fever has been sent to laboratory. To make serodiagnosis of typhoid fever with Widal test next antigen should be used:

Immune diagnostic serum for causative agent of typhoid fever
*Enteric fever diagnosticum
Alive pure culture of Salmonella
Erythrocytic salmonella diagnosticum
Serum of patient

88. A patient was hospitalized to an infectious department on the 8^th day of the disease complaining of headache, general weakness. Blood was taken for serological examination. Widal's reaction of agglutination appeared to be positive in dilution 1:200 with O-diagnosticum Salmonella typhi. What diagnosis can be made?

A. Cholera.
B. Shigellosis.
C. *Typhoid fever.
D. Leptospirosis.
E. Tuberculosis.

*A blood of patient with primary diagnosis “enteric fever” is examined in serological laboratory. After what time from onset of the most diseases serological method of diagnostics may be effective?

*After a week
After 3 days
After 12 hours
After a month
From onset of disease

*At serology (Widal test) of patient with enteric fever O- and H-agglutinins have been revealed in titers 1:800 and 1:200 respectively. It indicates about:

Recovery period
Having an illness before
State after immunization
*The onset of disease
Impossibility of diagnosis confirmation

b-12 During the repeated Widal's agglutination test it was noticed that the ratio of antibody titers and

O-antigens S.typhi in the patient's serum had increased from 1:100 to 1:400. How would
you interpret these results?