Bank of Krok testing to module N1

A Diphtheria
B Angina
C Scarlet fever
D Meningococcal nasopharyngitis
E Epidemic parotitis

* A pure culture was isolated from material (membrane from tonsils) of patient with suspected case of diphtheria. Morfologically these microorganisms are:

*Rods with clublike bulges on ends
Spherical shape
Rod-shaped
Spiral form
Rods with pointed ends

* From patient with suspected faucial diphtheria a bacterium with next morphological properties was isolated: Gram-positive rod with bulges onto ends, 3x0.6 µm, arranged as fence in smear. Which microorganism is it?

*Corynebacterium diphtheriae
Corynebacterium pseudodiphthericum
Streptococcus
Actynomyces
Bordetella pertussis

* During identification of Corynebacterium diphtheria culture cultural properties, starch fermentation and hemolytic activity are studied for:

*Indication of biovars
Indication of serovars
Detection of toxigenecity
Phage typing
Bacteriocin detection

b-12 A 4-year-old child presents with general weakness, sore throat and deglutitive problem. After his examination a doctor suspected diphtheria and sent the material to the bacteriological laboratory. In order to determine the diphtheria causative agent the material should be inoculated into the following differential diagnostic medium:

A Blood tellurite agar
B Endo's agar
C Ploskyrev's agar
D Sabouraud's agar
E Levenshtein-Yessen agar
*08* b-12 Inoculum from pharynx of a patient ill with angina was inoculated onto blood-tellurite agar. It resulted in growth of grey, radially striated (in form of rosettes) colonies 4-5 mm in diameter. Gram-positive bacilli with clublike thickenings on their ends placed in form of spread wide apart fingers are visible by microscope. What microorganisms are these?
*Diphtheria corynebacteria
Streptobacilli
Botulism clostridia
Streptococci
Diphtheroids

6. Prophylactic vaccination of a student's group was necessary because of a case of diphtheria. What preparation should be used to develop artificial active immunity?

A. *Diphtheria toxoid.
B. Antidiphtheritic serum.
C. Specific immunoglobulin.
D.Vaccine DPT (triple vaccine).
E. Inactivated vaccine.

*07*08* b-12 45. To determine the toxigenicity of diphtheria causative agents isolated from patients, cultures are inoculated onto a Petri dish with nutrient agar on both sides of the centrally located filter paper strip sodden with antidiphtheric antitoxic serum. After cultures incubation in the agar between separate cultures and filter paper strip the sites of medium opacity are revealed. Which serological reaction has been carried out?

A. Precipitation in gel.
B. Coombs reaction.
C. Agglutination.
D. *Ring-precipitation.
E. Opsonization.

*05* During investigation of preschool institute staff for carriage state C.diphtheriae was isolated from one of kindergarten teachers. Toxigenecity examination has been made and it nas been revealed that this strain did not produce exotoxine. Which reaction was made to detect toxigenecity of diphtheria bacteria?

Complement fixation test
Immunofluorescence test
Agglutination test
*Precipitation in agarose gel
Ring precipitation test

55. Pure culture of diphtheria corynebacteria is isolated from a patient. What serologic test should be used for detecting toxigenicity of the culture?

A. Agglutination.
B. *Precipitation in agar.
C. Complement fixation test.
D. Hemagglutination inhibition.
E. Passive hemagglutination.

102. Corynebacterium diphtheria was obtained from a sick child with diphtheria suspected. What research is necessary to be carried out to be convinced that this microbe causes diphtheria?

A. Agglutination test.
B.Staining by Burri-Giens' method.
C. Inoculation on blood agar.
D.Infecting a rabbit.
E.*Determining of toxigenicity

*07* 154. A girl of 7 with high temperature, complaints of pain in throat and general weakness has been hospitalized to the infectious department. The doctor has suspected diphtheria and has given the instruction to take material from the fauces for isolation of the pure culture of the pathogen. Which point is the most important for confirmation of the diagnosis «diphtheria»?

A. Detecting volutin granules in the causative agent.
B. *Toxigenicity test.
C.Cystinase test.
D. Hemolytic activity of the causative agent.
E.Phage typing.

b-12 From pharynx of a child with suspected diphtheria a pure culture of microorganisms was

isolated. Their morphological, tinctorial, cultural and biochemical properties appeared to be
typical for diphtheria causative agents. What study should be conducted in order to drow a
conclusion that this is a pathogenic diphtheria bacillus?