Gen om e d iver si ty

2013
d
öll
, S.: Studies on scopolin and scopoletin – stress-induced ac-
cumulation and regulatory elements. (Phd thesis) Martin-
Luther-universität halle-Wittenberg, naturwissenschaftli-
che Fakultät i biowissenschaften, halle/S. (2013) 196 pp.
P
eukert
,  M.: Spatiotemporal distributions of metabolites in-
volved in barley grain development with emphasis on en-
dosperm formation. (Phd thesis) Martin-Luther-universität 
halle-Wittenberg, naturwissenschaftliche Fakultät i biowis-
senschaften, halle/S. (2013) 196 pp.
W
ang
,  d.: identifizierung von Metaboliten der Gerste zur 
charakterisierung der Salzstressabwehr. (bachelor thesis) 
hochschule anhalt (Fh), köthen (2013) 77 pp. (hochschul-
betreuer aus dem iPk: Prof. G. kunze, dr. u. Scholz).
F
ila
,  J., a. M
atros
, S. R
adau
, R.P. z
ahedi
, V. Č
apková
, h.P. M
ock
 &  
d. h
onys
: Revealing phosphoproteins playing role in to-
bacco pollen activated in vitro. Proteomics 12 (2012) 3229-
3250.
J
aniak
,  a., S. P
iórko
, a. M
atros
, h.P. M
ock
, M. k
waśniewski
,  
k. c
hwialkowska
, b. c
hmielewska
 & i. S
zarejko
: a comparative 
analysis of proteins that accumulate during the initial stage 
of root hair development in barley root hair mutants and 
their parent varieties. J. appl. Genet. 53 (2012) 363-376.
M
elonek
, J., a. M
atros
, M. t
rösch
, h.P. M
ock
 & k. k
rupinska
: the core 
of chloroplast nucleoids contains architectural SWib do-
main proteins. Plant cell 24 (2012) 3060-3073.
P
eukert
, M., a. M
atros
, G. L
attanzio
, S. k
aspar
, J. a
badia
 & h.P. M
ock
: 
Spatially resolved analysis of small molecules by matrix-
assisted laser desorption/ionization mass spectrometric 
imaging (MaLdi-MSi). new Phytol. 193 (2012) 806-815.
R
iewe
, d., M. k
oohi
, J. L
isec
, M. P
feiffer
, R. L
ippmann
, J. S
chmeichel
, L. 
W
illmitzer
 & t. a
ltmann
: a tyrosine aminotransferase involved 
in tocopherol synthesis in Arabidopsis. Plant J. 71 (2012) 
850-859.
2013
b
arkla
, b.J., t. c
astellanos
-c
ervantes
, J.L. d
iaz
 
de
 L
eón
, a. M
atros
, h.P. 
M
ock
, F. P
erez
-a
lfocea
, G.h. S
alekdeh
, k. W
itzel
 & c. z
örb
: eluci-
dation of salt stress defense and tolerance mechanisms of 
crop plants using proteomics – current achievements and 
perspectives. Proteomics 13 (2013) 1885-1900.
G
utierrez
-c
arbonell
, e., G. L
attanzio
, R. S
agardoy
, J. R
odríguez
-c
elma
, 
J.J. R
íos
 R
uiz
, a. M
atros
, a. a
badía
, J. a
badía
 & a.F. L
ópez
-M
illán
: 
changes induced by zinc toxicity in the 2-de protein profile 
of sugar beet roots. J. Proteomics 94 (2013) 149-161.
L
attanzio
, G., S. a
ndaluz
, a. M
atros
, J.J. c
alvete
, J. k
ehr
, a. a
badía
, J. 
a
badía
 & a.F. L
ópez
-M
illán
: Protein profile of Lupinus texensis 
phloem sap exudates: Searching for Fe- and zn-containing 
proteins. Proteomics 13 (2013) 2283-2296.
M
atros
, a. & h.P. M
ock
: Mass spectrometry based imaging tech-
niques for spatially resolved analysis of molecules. Front. 
Plant Sci. 4 (2013) 89.
M
uthuramalingam
, M., a. M
atros
, R. S
cheibe
, h.P. M
ock
 & k.J. d
ietz
: 
the hydrogen peroxide-sensitive proteome of the chloro-
plast in vitro and in vivo. Front. Plant Sci. 4 (2013) 54.
P
eukert
, M., S. W
eise
, M.S. R
öder
 & i.e. M
atthies
: development of 
SnP markers for genes of the phenylpropanoid pathway 
and their association to kernel and malting traits in barley. 
bMc Genet. 14 (2013) e97.
t
rautwein
-S
chult
, a., d. J
ankowska
, a. c
ordes
, P. h
oferichter
, c. k
lein
, 
a. M
atros
, h.P. M
ock
, k. b
aronian
, R. b
ode
 & G. k
unze
: Arxula  
adeninivorans recombinant urate oxidase and its applica-
tion in the production of food with low uric acid content.  
J. Mol. Microbiol. biotechnol. 23 (2013) 418-430.

Abteilung Physiologie und Zellbiologie/
Department of Physiology and Cell Biology
132
z
latina
,  k.: Proteomanalysen des kavernensaftes in Gersten-
samen. (bachelor thesis) Martin-Luther-universität halle-
Wittenberg, naturwissenschaftliche Fakultät i biowissen-
schaften, halle/S. (2013) 45 pp.
z
wies
,  c.: untersuchungen zum Phosphoproteinmuster von 
Gerstenwurzeln bei Salzstress. (bachelor thesis) Martin-Lu-
ther-universität halle-Wittenberg, naturwissenschaftliche 
Fakultät i biowissenschaften, halle/S. (2013) 45 pp.

133
monitoring of cell dynamic processes and the spatial distribu-
tion of macromolecules in plants using sophisticated cell bio-
logical techniques.
Research Report
Pollen embryogenesis is a powerful tool to produce 
haploid and doubled haploid plants. in collaboration with 
the research groups Plant Reproductive biology (J. kumlehn) 
and chromosome Structure and Function (a. houben) we 
continued the search for underlying mechanisms using 
barley as a model plant (P. Pandey). Since gene expression 
is influenced by epigenetic modifications we hypothesized 
that the latter might be a driving force behind induction. by 
means of immunolabeling we first documented the dynamic 
epigenetic modifications during pollen gametogenesis, which 
confirmed active gene expression in the vegetative cell and 
overall silencing in the generative cell (Pandey 2013). Within  
24 h after the start of induction of pollen embryogenesis 
profound redistributions were observed in the chromatin 
modifications h3k9ac, h3k4me2, and h3k27me3 which play 
a profound role in regulating transcriptional activity (Fig. 
40). the results suggest an important role for epigenetic 
modifications in pollen embryogenesis. Since the 
modifications take place before first pollen mitosis, they are 
also the earliest indicators for successful pollen induction.
Research Group: Structural Cell Biology
head:  dr. Michael Melzer
Scientists
IPK financed
Rutten, twan, dr.
Grant Positions
Muszyńska, aleksandra (0,50 industry, since 15.06.2012)
Visiting Scientists/Scholars
daghma, diaa el-din, dr. (self-financed, 07.10.-12.10.2013) 
Marzec, Marec (daad, 11,04.-11.05.2012, 01.06.-25.06.2012, 
23.10.-08.11.2012, 28.11.-21.12.2012, 02.03.-26.03.2013; self-
financed, 15.07.-21.08.2013)
kothari, S. L., Prof. (dFG/inSa-Programme, 09.02.-01.03.2012)
Pandey, Pooja (daad Siemens Fellowship)
Prokhorenko, isabella R., Prof. (self-financed, 11.07.-12.08.2013)
tayyab, Muhammad (Scholarship higher education commis-
sion Pakistan, 08.11.2012-03.08.2013)
Goals
as core facility for light and electron microscopy we perform 
histological and ultrastructural analysis of metabolic and de-
velopmental processes in plants. our studies which comprise 
both own research projects, as well as internal and external 
co-operations, focus mainly on ultrastructural characterization, 
Fig. 40
distribution of histone h3k27me3 (histone3 trimethylated at position lysine 27) in pollen of Hordeum vulgare using flourescence immunolabeling in combination with 
confocal laser scanning microscopy. Shortly after first pollen mitosis florescence signal in gametophytic pollen is restricted to specific domains in both nuclei (A), while 
after induction of embryogenesis histone h3k27me3 labeling is present throughout the cytoplasm but absent from the nuclei (B). arrows indicate the position of nuclei. 
cyt, cytoplasm (P. Pandey, t. Rutten, J. kumlehn, a. houben and M. Melzer).

Abteilung Physiologie und Zellbiologie/
Department of Physiology and Cell Biology
134
Fig. 41
Structural characterisation of 
cross sections of basal inter-
nodes of wildtype (R1124) and 
'Stabilstroh' (ms135). (A, B) 
Phloroglucinol staining of 150 
µm thick vibratome sections of 
fresh material reveals enhanced 
lignin content (red) and more 
pronounced stalk invaginations 
in ‘Stabilstroh’. (C, D) Scanning 
electron microscopy images 
of straw. (E, F) Fluorescence 
microscopy images of 2 µm 
thick sections of Spurr's resin 
embedded tissue stained with 
basic fuchsine (eX bP 546/12, 
bS Ft 560, eM bP 575-640). (G-J) 
transmission electron micros-
copy images of sclerenchyma 
(G, I) and parenchyma (H, J) 
shows significantly thicker cell 
walls in ms135. cw, cell wall; ML, 
middle lamella; Pa, parenchy-
ma; Sc, sclerenchyma; Si, stalk 
invagination; Vb, vascular bun-
dle (a. Muszyńska, a. börner,  
M. Röder and  M. Melzer). 
wildtype (R1124)
'Stabilstroh' (ms135)

135
under investigation by the research group Plant architecture 
(h. Youssef). SeM analysis of pike development has become an 
essential aspect for identifying the development and extent of 
labile features in plant lines under investigation
in a common project (daad) with the dept. of Genetics, kato-
wice, Poland (i. Szarejko, M. Marzec) we continued working on the 
structural and molecular characterisation of root epidermal 
cells for the identification of new genes involved in root hair de-
velopment in Hordeum vulgare. using histological and cell biolo-
gical methods the system of the cell pattern of barley root epider-
mis was investigated. obtained results showed that root hair dif-
ferentiation starts with an asymmetric expansion of the daughter 
cells arising from a symmetric division. a central element appears 
to be the symplasmic communication between root epidermal 
cells which becomes restricted during cell specialisation. We ob-
served that in mutant lines lacking root hair differentiation this 
symplasmic isolation does not occur. immunolocalisation studies 
of the arabinogalactan-protein (aGP) epitopes (LM2, LM14, and 
Mac207) showed that at least three different classes of aGPs are 
involved in root hair development in barley.
Publications
Peer Reviewed Papers
2012
a
garwal
, R., G. M
aralihalli
, V. S
udarsan
, S.d. c
houdhury
, R.k. V
atsa
, 
h. P
al
, M. M
elzer
 & J.k. S
ainis
: differential distribution of pig-
ment-protein complexes in the thylakoid membranes of 
Synechocystis 6803. J. bioenerg. biomembr. 44 (2012) 399-
409.
a
gueci
, F., t. R
utten
, d. d
emidov
 & a. h
ouben
: Arabidopsis atnek2 
kinase is essential and associates with microtubules. Plant 
Mol. biol. Rep. 30 (2012) 339-348.
c
eccoli
,  R.d., n.e. b
lanco
, M.e. S
egretin
, M. M
elzer
, G.t. h
anke
, R. 
S
cheibe
, M.R. h
ajirezaei
, F.F. b
ravo
-a
lmonacid
 & n. c
arrillo
: Fla-
vodoxin displays dose-dependent effects on photosynthe-
sis and stress tolerance when expressed in transgenic to-
bacco plants. Planta 236 (2012) 1447-1458.
d
aghma
,  d.S., J. k
umlehn
, G. h
ensel
, t. R
utten
 & M. M
elzer
: time-
lapse imaging of the initiation of pollen embryogenesis in 
barley (Hordeum vulgare L.). J. exp. bot. 63 (2012) 6017-6021.
h
edtke
, b., a. a
lawady
, a. a
lbacete
, k. k
obayashi
, M. M
elzer
, t. R
oitsch
, 
t. M
asuda
 & b. G
rimm
: deficiency in riboflavin biosynthesis 
affects tetrapyrrole biosynthesis in etiolated Arabidopsis tis-
sue. Plant Mol. biol. 78 (2012) 77-93.
h
ensel
, G., S. o
leszczuk
, d.e. d
aghma
, J. z
imny
, M. M
elzer
 & J. k
um
-
lehn
: analysis of t-dna integration and generative segrega-
tion in transgenic winter triticale (x triticosecale Wittmack). 
bMc Plant biol. 12 (2012) 171.
J
unker
, a., G. M
önke
, t. R
utten
, J. k
eilwagen
, M. S
eifert
, t.M. t
hi
, J.P. 
R
enou
, S. b
alzergue
, P. V
iehover
, u. h
ähnel
, J. L
udwig
-M
üller
, L. 
a
ltschmied
, u. c
onrad
, b. W
eisshaar
 & h. b
äumlein
: elongation-
related functions of LeaFY cotYLedon1 during the devel-
opment of Arabidopsis thaliana. Plant J. 71 (2012) 427-442.
Lodging, the state of permanent displacement of the tillers 
from their upright position, is a main concern in crop produc-
tion as it decreases grain quality and increases the costs of 
harvesting.  Lodging resistance is the most important objec-
tive in rye breeding. in a joint project with dieckmann Gmbh & 
co. kG, Monsanto Saaten Gmbh and the research groups Gene 
and Genome Mapping (M. Röder), and Resources Genetics and 
Reproduction (a. börner) we focus on the molecular genetic 
and histological characterisation of the trait 'Stabilstroh' 
(a. Muszyńska). this recently identified genotype of rye has the 
best lodging resistance and simultaneously is characterised by 
the longest tillers among the German collection of rye hybrids. 
to identify the genetic traits histological and ultrastructural 
studies were performed on the basal internodes of segregating 
F
2
 population (‘304/1’) and its parental lines (see Fig. 41, p. 134). 
‘Stabilstroh’ was found to have a significantly higher sclerenchy-
ma/parenchyma ratio while both sclerenchyma cell walls and 
inner periclinal cell walls of the epidermis are clearly thickened 
in comparison to the wildtype. 'Stabilstroh' is also characterised 
by a high number of pronounced stalk invaginations, an impor-
tant factor in enhancing mechanical stability. these parameters 
affecting mechanical stability of tillers are currently being used 
to find Quantitative Trait Loci (QTLs) underlying these dis-
tinct morphological characters.
to gain deeper insight into determinants of salt tolerance we 
continued our immunological studies of the localisation of 
horculin in roots of the salt-tolerant line Morex and the salt-
sensitive line Steptoe in a joint project of the department of 
Physiology and cell biology.
to improve the understanding of the regulation of biologi-
cal processes in plants is a major aspect of numerous internal 
and external cooperations. together with the research group 
Molecular Plant nutrition (M.R. hajirezaei) the morphological 
characterisation of Petunia hybrida shoot tip cuttings ex-
posed to various nutrient supplies was continued with a strong 
emphasis on hormone effect, carbohydrate metabolism and 
adventitious root formation. 
in close collaboration with the research group Quantitative 
Genetics (i. Lermontova) the distribution and dynamics of  
the CENH3 binding proteins KNL2 and SIM3 were investi-
gated in root tip cells of Arabidopsis thaliana. using YFP-tagged 
constructs and life cell imaging knL2 was found located to 
centromeres throughout the cell cycle until prophase. during 
mitosis knL2 circulated in the cytoplasm. after the nuclear 
membranes reformed, knL2 first reentered the nucleoplasm 
followed by the centromeres. the distribution of SiM3, which 
is also involved in cenh3 assembly, resembles that of knL2, ex-
cept for the absence of an accumulation at centromeres. both 
proteins are highly mobile with recovery times after bleaching 
being about five times less for SiM3 than those for knL2. 
the six-rowed spike phenotype, which has the potential to 
significantly increase grain yield in barley, is under the control 
of at least five different genes. intermediate forms, which show 
a mixture of both six- and two-rowed phenotype have pointed 
to the existence of yet another factor, the lab-gene, which is 

Abteilung Physiologie und Zellbiologie/
Department of Physiology and Cell Biology
136
k
oppolu
,  R., n. a
nwar
, S. S
akuma
, a. t
agiri
, u. L
undqvist
, M. P
our
-
kheirandish
, t. R
utten
, c. S
eiler
, a. h
immelbach
, R. a
riyadasa
, h.M. 
Y
oussef
, n. S
tein
, n. S
reenivasulu
, t. k
omatsuda
 & t. S
chnurbusch
: 
Six-rowed spike4 (Vrs4) controls spikelet determinacy and 
row-type in barley. Proc. natl. acad. Sci. u.S.a. 110 (2013) 
13198-13203.
L
ermontova
,  i., M. k
uhlmann
, S. F
riedel
, t. R
utten
, S. h
eckmann
, M. 
S
andmann
, d. d
emidov
, V. S
chubert
 & i. S
chubert
: Arabidopsis ki-
netochoRe nuLL
2
 is an upstream component for cenh3 
deposition at centromeres. Plant cell 25 (2013) 3389-3404.
M
arzec
, M., M. M
elzer
 & i. S
zarejko
: asymmetric growth of root 
epidermal cells is related to the differentiation of root hair 
cells in Hordeum vulgare L., J. exp. bot. 64 (2013) 5145-5155.
M
arzec
, M., a. M
uszyńska
 & d. G
ruszka
: the role of strigolactones 
in nutrient-stress responses in plants. int. J. Mol. Sci. 14 
(2013) 9286-304.
M
au
, M., J.M. c
orral
, h. V
ogel
, M. M
elzer
, J. F
uchs
, M. k
uhlmann
, n. 
de
  S
torme
, d. G
eelen
 & t.F. S
harbel
: the conserved chimeric 
transcript UPGRADE-2 is associated with unreduced pollen 
formation and is exclusively found in apomictic Boechera. 
Plant Physiol. 163 (2013) 1640-1659
.
P
andey
, P., a. h
ouben
, J. k
umlehn
, M. M
elzer
 & t. R
utten
: chromatin 
alterations during pollen development in Hordeum vulgare. 
cytogenet. Genome Res. 141 (2013) 50-57.
R
ubtsova
,  M., h. G
nad
, M. M
elzer
, J. W
eyen
 & M. G
ils
: the auxins 
centrophenoxine and 2,4-d differ in their effects on non-
directly induced chromosome doubling in anther culture of 
wheat (T. aestivum L.). Plant biotechnol. Rep. 7 (2013) 247-
255.
z
bierzak
, a.M., S. P
orfirova
, t. G
riebel
, M. M
elzer
, J.e. P
arker
 & P. d
ör
-
mann
: a tiR-nbS protein encoded by Arabidopsis  Chilling 
Sensitive 1 (CHS1) limits chloroplast damage and cell death 
at low temperature. Plant J. 75 (2013) 539-552.
Books and Book Chapters
2013
a
garwal
, R., a. M
atros
, M. M
elzer
 & h.P. M
ock
: blue-native page 
analysis validates heterogeneity in the thylakoids of Syne-
chocystis 6803. in: k
uang
, t., c. L
u
 & L. z
hang
 (eds.): Photosyn-
thesis Research for Food, Fuel and the Future. 15
th
 int. conf. 
Photosynthesis. (Series: advanced topics in Science and 
technology in china). Springer, berlin-heidelberg (2013) 
385-388.
k
asaras
, a., M. M
elzer
 & R. k
unze
: Arabidopsis senescence-associ-
ated protein dMP1 is involved in membrane remodeling of 
the eR and tonoplast. bMc Plant biol. 12 (2012) 54.
M
eyer
,  R.c., h. W
itucka
-W
all
, M. b
echer
, a. b
lacha
, a. b
oudichevs
-
kaia
, P. d
örmann
, o. F
iehn
, S. F
riedel
, M. 
von
  k
orff
, J. L
isec
, M. 
M
elzer
, d. R
epsilber
, R. S
chmidt
, M. S
cholz
, J. S
elbig
, L. W
illmitzer
 
& t. a
ltmann
: heterosis manifestation during early Arabidop-
sis seedling development is characterized by intermediate 
gene expression and enhanced metabolic activity in the 
hybrids. Plant J. 71 (2012) 669-683.
R
adchuk
,  V., J. k
umlehn
, t. R
utten
, n. S
reenivasulu
, R. R
adchuk
, h. 
R
olletschek
, c. h
errfurth
, i. F
eussner
 & L. b
orisjuk
: Fertility in 
barley flowers depends on Jekyll functions in male and fe-
male sporophytes. new Phytol. 194 (2012) 142-157.
S
edzielewska
,  k.a., e. b
öer
, c. b
ellebna
, t. W
artmann
, R. b
ode
, M. 
M
elzer
, k. b
aronian
 & G. k
unze
: Role of the AFRD1 encoded fu-
marate reductase in hypoxia and osmotolerance in Arxula 
adeninivorans. FeMS Yeast Res. 12 (2012) 924-937.
t
hiel
, J., J. h
ollmann
, t. R
utten
, h. W
eber
, u. S
cholz
 & W. W
eschke
: 454 
transcriptome sequencing suggests a role for two-compo-
nent signalling in cellularization and differentiation of bar-
ley endosperm transfer cells. PLoS one 7 (2012) e41867.
t
hiel
, J., d. R
iewe
, t. R
utten
, M. M
elzer
, S. F
riedel
, F. b
ollenbeck
, W. 
W
eschke
 & h. W
eber
: differentiation of endosperm transfer 
cells of barley - a comprehensive analysis at the micro-scale. 
Plant J. 71 (2012) 639-655.