Kniga na apstrakti book of abstrcts

Accumulation of toxic metals in various mushroom species 
from different regions of the Republic of Macedonia 
Olga Kirovska Cigulevska
1
; Biljana Bauer Petrovska
2
; Mitko Karadelev
3
1
JZO ZZZ Skopje, Treta Mak. Brigada 18; 
2
Faculty of Pharmacy, University of “Ss Ciryl and Methodius“, Vodnjanska 17; 
3
Institute of Biology, faculty of Natural Sciences and Mathematics, 
University of “Ss Ciryl and Methodius“, Gazi Baba bb, P.O.Box 162; 1000 Skopje, R Macedonia
The fact that mushrooms can accumulate metals from toxical aspect motivate us for this investigation, from
a point of establishing of the situation with the accumulation of toxic metals in the ecologicaly pure areas – high
mountanious places.
Acumaulation of toxic metals was analyzed in eleven mushroom species collected from different moun-
tanous regions of the Republic of Macedonia. Determination of the concentration of Pb, Cd, Zn and Cu was made
with the aid of atomic absorption spectrometry on the instrument Optima-QES 2000.
Results expressed on dry mass basis indicate that the content of Cu in the analysed mushroom samples ranged
from 0.081 mg/kg in Calocybe gambosa (Fr.) Donk to 0.7 mg/kg in Amanita caesarea (Scop.) Pers. mg/kg, with a
mean of 0.37 mg/kg. Zn values between 0.12 mg/kg in Lactarius deliciosus Fr. and 0.85 mg/kg in Auricularia auric-
ular-judae (Bull.:St.Anm.), were obtained. Obtained low concentrations of toxic metals Cu and Zn in the investigat-
ed samples indicated that this mushroom can be used as an additional supplement of these essential elements in
human nutrition.
Cd and Pb were found in 54 % of the investigated samples. The estimated Pb concentration ranged from
0.02 mg/kg in Tricholoma terreum (Schaeff.) Quel.to 0.08 mg/kg in Agaricus campester (L.)Fr.. In samples were Cd
was detected the concentrations were low and varied from 0.01 mg/kg in Amanita caesarea (Scop.) Pers. to 0.08
mg/kg in Boletus edulis Bull. In the samples where Cd and Pb were detected the average values were lower than the
maximum concentrations imposed by regulations.
All of the investigated toxic metals are present in extremely low concentrations in the investigated mush-
rooms samples. This proved the ecological purity of the regions where the mushrooms were collected. 
Macedonian pharmaceutical bulletin 53 (1,2) 334-335 (2007)
PP - 172
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Akumulirawe na toksi~ni metali vo razni vidovi gabi 
od pove}e regioni vo Republika Makedonija
Olga Kirovska Cigulevska
1
; Biljana Bauer Petrovska
2
; Mitko Karadelev
3
1
JZO ZZZ Skopje, Treta Mak. Brigada 18; 
2
Farmacevtski fakultet, Univerzitet „Sv. Kiril i Metodij“, Vodwanska 17; 
3
Institut za Biologija, Prirodno - matemati~ki fakultet, 
Univerzitet „Sv. Kiril i Metodij“, Gazi Baba bb, P. Fah 162; 1000 Skopje, R Makedonija
Faktot deka gabite mo`at da akumuliraat metali od toksikolo{ki aspekt go pobudi ova istra-
`uvawe so cel da se utvrdi sostojbata so akumuliraweto na toksi~ni metali vo ekolo{ki ~isti reoni -
visoki planinski mesta.
Akumuliraweto na toksi~ni metali e istra`uvano vo edinaeset razli~ni vidovi gabi od pove}e
planinski regioni na Republika Makedonija. Odreduvaweto na koncentracijata na elementite: olovo,
kadmium, cink i bakar e napraveno so metodot na atomska emisiona spektrometrija na instrument Optima-
QES 2000.
Rezultatite od istra`uvaweto iska`ani na suvite gabi poka`uvaat deka sodr`inata na bakar vo
analiziranite primeroci na gabi e vo granici od 0.081 mg/kg vo Calocybe gambosa (Fr.) Donk do 0.7 mg/kg vo
Amanita caesarea (Scop.) Pers. mg/kg, prose~no 0.37 mg/kg. Koncentracijata na cink iznesuva od 0.12 mg/kg vo
Lactarius deliciosus Fr. do 0.85 mg/kg vo Auricularia auricular-judae (Bull.:St.Anm.). Toksi~nite elementi bakar i
cink se prisutni vo niski koncentracii vo istra`uvanite primeroci {to uka`uva na toa deka istite
mo`at da se upotrebat kako dopolnitelen izvor na ovie esencielnite elementi vo ishranata. 
Vo 54 % od ispituvanite primeroci na gabi  doka`ano e prisustvo na olovo i kadmium. Najdenata
sodr`inata na olovo e vo granici od 0.02 mg/kg vo Tricholoma terreum (Schaeff.) Quel. do 0.08 mg/kg vo Agaricus
campester (L.)Fr. Vo primerocite kade be{e pronajden kadmium koncentracijata e niska i se dvi`i vo grani-
ci od 0.01 mg/kg vo Amanita caesarea (Scop.) Pers. do 0.08 mg/kg vo Boletus edulis Bull. Vo site ispituvani prime-
roci najdenite vrednostite na olovo i kadmium bea poniski od maksimum so zakon dozvoleni koncentracii.
Site toksi~ni elementi vo suvite gabi se prisutni vo isklu~itelno niski koncentracii so {to
se potvrduva i ekolo{kata ~istota na regionite kade {to istite se sobrani. 
Macedonian pharmaceutical bulletin 53 (1,2) 334-335 (2007)
PP - 172
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Chemical composition of the essential oils of three Micromeria
species growing in Macedonia and Bulgaria
Emanuela Kostadinova
1
, Kalina Alipieva1, Marina Stefova
2
, Trajce Stafilov
2
, Daniela Antonova
1
, 
Ljuba Evstatieva
3
, Vlado Matevski
4
, Svetlana Kulevanova
5
, Gjoshe Stefkov
5
, Vassya Bankova
1
1
Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria 
2
Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Skopje, R. Macedonia
3
Institute of Botany, Bulgarian Academy of Sciences, Sofia, Bulgaria 
4
Institute of Biology, Faculty of Natural Sciences and Mathematics, Skopje, R. Macedonia
5
Institute of Pharmacognosy, Faculty of Pharmacy, Skopje, R. Macedonia
The essential oils from three species of genus Micromeria Bentham (fam. Lamiaceae) growing wild in
Bulgaria (M. dalmatica and M. cristata) and Macedonia (M. cristata and M. juliana) have been analyzed and dis-
cussed in context with the results obtained for the corresponding Micromeria essential oils from the region. The
obtained essential oils were analyzed by GC-MS and components were identified according to their mass spectra
and linear retention indices.
M. dalmatica was characterized by high quantities of essential oil and oxygenated monoterpenes with p-
menthane skeleton were the main constituents. The two samples of M. cristata from Bulgaria showed significant dif-
ferences in their chemical composition (monoterpenic alcohols prevailed in the sample from Bosnek, whereas
sesquiterpenes dominated in the oil from plants of the Ilindentsi population). Very similar content of total monoter-
penes, oxygenated monoterpenes and sesquiterpenes was found in the essential oil of M. cristata from Alshar,
Macedonia, and from Ilindentsi, Bulgaria, However, these two samples differed substantially in the variety of iden-
tified sesquiterpene compounds (20 compared to 5 and 4 in the other M. cristata oil samples). High content of
sesquiterpenes was also found in the essential oil of M. juliana from Macedonia with the distribution pattern in accor-
dance with the data found for other samples of M. juliana from the region.
Macedonian pharmaceutical bulletin 53 (1,2) 336-337 (2007)
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Hemiski sostav na eteri~nite masla od tri vida 
Micromeria koi rastat vo Makedonija i Bugarija
Emanuela Kostadinova
1
, Kalina Alipieva
1
, Marina Stefova
2
, Traj~e Stafilov
2
, Daniela
Antonova
1
, Quba Evstatieva
3
, Vlado Matevski
4
, Svetlana Kulevanova
5
, 
\o{e Stefkov
5
, Vasja Bankova
1*
1
Institut za organska hemija so Centar za fitohemija, Bugarska akademija na naukite, Sofija, Bugarija 
2
Institut za hemija, Prirodno-matemati~ki fakultet, Skopje, R. Makedonija
3
Institut za botanika, Bugarska akademija na naukite, Sofija, Bugarija
4
Institut za biologija, Prirodno-matemati~ki fakultet, Skopje, R. Makedonija
5
Institut za farmakognozija, Farmacevtski fakultet, Skopje, R. Makedonija
Eteri~nite masla od tri vida od rodot Micromeria Bentham (fam. Lamiaceae) koi rastat vo Bugarija
(M. dalmatica i M. cristata) i Makedonija (M. cristata and M. juliana) se analizirani i rezultatite se diskuti-
rani vo kontekst so rezultatite dobieni za eteri~ni masla od soodvetnite vidovi Micromeria od regionot.
Dobienite eteri~ni masla se analizirani so gasna hromatografija-masena spektrometrija i identifikaci-
jata na komponentite e izvr{ena spored nivnite maseni spektri i linearni retencioni indeksi.
M. dalmatica se karakterizira{e so golemo koli~estvo od eteri~no maslo, vo koe kislorodnite
monoterpeni so mentanski skelet se dominantni komponenti. Dvata primeroka od M. cristata od Bugarija
zna~itelno se razlikuvaa vo hemiskiot sostav (monoterpenskite alkoholi dominiraa vo primerokot od
Bosnek, dodeka seskviterpenite bea dominantni komponenti vo primerokot od Ilindenci). Sli~na
sodr`ina na vkupni monoterpeni, kislorodni monoterpeni i seskviterpeni be{e najdena i vo eteri~noto
maslo od M. cristata od Al{ar, Makedonija, i vo ona od Ilindenci, Bugarija. Sepak, ovie dva primeroci
zna~itelno se razlikuvaa po brojot na identifikuvani seskviterpenski soedinenija (20 sporedeno so 5 i
4 soedinenija vo drugite dve eteri~ni masla od M. cristata). Golemo koli~estvo od seskviterpeni be{e, isto
taka, najdeno i vo eteri~noto maslo od M. juliana od Makedonija, a raspredelbata na seskviterpenskite kom-
ponenti be{e vo soglasnost so onaa za analogni primeroci od regionot.
Macedonian pharmaceutical bulletin 53 (1,2) 336-337 (2007)
PP - 173  
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Pharmacy from XIII to XVI century
Skrbo A., Sober M., Marjanovic A., Djedjibegovic J.
Faculty of Pharmacy, University of Sarajevo, Cekalusa 90, Sarajevo, Bosnia and Herzegovina
For the renaissance of clasicall medicine and pharmacy most responsible are Arabs, Persians, Syrians and Jews.
Great campaign of Arabs begun in 7
th
century. They seized whole Midlle East, Persia, Egypt, and afterwards Spain,
India, Constantinople, Balkan and Hungary all over to Wienna. Arabian doctors and pharmacists, in the period from
VIII to XVI century, had important role in development of pharmacy: They have opened first pharmacies in Europe.
The very first pharmacy was opened in Salerno, than in Cordoba and Seville and afterwards in other european cities.
In Salern’s edict that was brought in 1240. medicine and pharmacy were formaly divided, but pharmacists
and doctors stayed in specific interrelation for centuries. In Europe, first pharmacies and laws about them exsisted
in Venice in 1258. and Florence in 1300. They were opened pattern to arabian pharmacies, first pharmacies with not
only commercial but also with scientific attribute, because first pharmacists were chemists, astrologists, magicians
and merchants.   
A great progress has been made in pharmacy during XVI century. Major role in that progress had Paracelsus
(1493-1541). He pioneered the use of chemicals and minerals in medicine. He was the most notable leader of iatro-
chemistry, that had great influence on medical treatment. Pharmacy at Balkan peninsula arose in late XIII century.
It was under great influence of western medicine at that time, that was reached from Republic of Dubrovnik and oth-
ers cities by the Adriatic coast. During the period of independent Bosnian state mostly traditional medicine and phar-
macy were present. Many barbers were practicing pharmacy, preparing and selling drugs, mostly balsams. Traditional
medicine founded explanation for causes of various illnesses in ocult world of demons, so one of the ways to cure
was divination.
After collapse of independent Bosnian state at the end of XV century, domination of Ottoman Empire begun.
Influence of the East was evident especially in medicine. Ottomans brought rich threasury of Arabian medicine and
pharmacy to Bosnia, with all its prescriptions and literature. Unfortunatelly, it did not improve public health since
almost anyone could practice medicine and pharmacy.
Macedonian pharmaceutical bulletin 53 (1,2) 338 (2007)
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„ATTARI“ and „LJEKARUSE“ – their contribution 
to development of pharmacy
Skrbo A., Sober M., Marjanovic A., Djedjibegovic J.
Faculty of Pharmacy, University of Sarajevo, Cekalusa 90, Sarajevo, Bosnia and Herzegovina
In XVI century in Bosnia and Herzegovina first attar's shops were set up and people who were opened them
were called „attari“. The word „attar' is Arabian-Turkish origin, and the first meaning of this word was „area around
settlements that was used by the field warders“, but the most common meaning is „the man that sells herbs and drugs“.
The term „attar“ can also be related to arabian word „itr“ which mean spice. First attars were Jews, Jermenians and
Muslims. The first Jewish attar's shops were opened at Bascarsija in 1565, where herbs, spices, etheric oils and drugs
were sold. All data about drugs from this period gives Materia medica of attar's age, lists of inventory, papers so called
„ljekarusse“ etc. From the lists of drugs that were found in these shops not only the influence from East and West but
also the influence of alchemists and iatrochemists from that age can be seen. Educated people from that age, and after-
wards attars were translating and transcripting old medical books, recording their recipes with translations and in that
manner “jekaruse“ were made. By their origin and languages on which they were written ljekaruse can be divided on
Muslim, Jewish, Serbian and Croatian (Franciscan). They were written in Latin, ladino-Hebrew, Italian, Turkish and
Arabian. In Sarajevo first attars were Jewish. Most important documents from that period are two manuscripts that
were so far in the possession of Jewish municipality. These manuscripts comprise recipes for drugs and medical prepa-
rations, elements of spiritual rituals and romances that were written in Spanish-Hebrew language called „rashi“.
„Ljekaruse“ that were written in Serbian origin from the monks and orthodox priests. These manuscripts contain recipes
from Byzantine Greek and Arabian medicine with elements of spiritual rituals. Most of the manuscripts in Croatian
were written by Bosnian Franciscans and are mostly remained in friaries.
Attars and their manuscripts-“ljekaruse“ had great influence on development of medicine and pharmacy in
Bosnia and Herzegovina, especially on establishment of herbal shops and pharmacies.
Macedonian pharmaceutical bulletin 53 (1,2) 339 (2007)
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340

341
^ETVRTI KONGRES NA FARMACIJATA NA MAKEDONIJA SO ME\UNARODNO U^ESTVO
FOURTH CONGRESS OF PHARMACY OF MACEDONIA WITH INTERNATIONAL PARTICIPATION
A
Acevska L.
328
Adamova E.
67
Ancevska Netkovska K.
246
Anevska M.
67
Anevska-Stojanovska N.
51
Angelova S.
263
Antova D.
118
Antonijevic B.
261
Adamovska E.
109
Ajanovska V.
223
Akimovska M.
239
Aleksic M.
173, 185
Aleksic D.
124, 125
Alipieva K.
336
Alomerovic A.
243
Angeleska S.
90
Angelovska B.
130, 132
Antic Stankovic J.
284
Antonova D.
336
Antunovic M.
124, 125
Apostolski S.
149, 151
Apostolovska S.
226
Arica B.
50
Arsic D.
318, 319
Arsic I.
73, 320
Arsova-Sarafinovska Z.
162, 166, 244  
Atik Bekkara F.
311
Atanackovic M.
316, 
Atanackovic J.
185
Avdic N.
189
B
Babic Z.
80
Babunovska H.
209, 211, 213, 223, 231
Bagasovska E.
265
Banev S.
162, 166
Bankova V.
336
Batkovska Borozanova I.
215
Bauer Petrovska B.
278, 280, 296, 330, 332, 334
Becic F.
143
Begovic A.
239
Benhammou N.
311
Betz G.
63, 64, 65, 66, 83
Bjelanovic J.
111
Blagojevic B.
318, 319
Blazevska I. 
109
Bochot A.
35
Bogdanova M.
259, 290, 292, 294
Bogdanov J.
213
Bojo-Omeragic S.
71
Bondzulic B.
317
Boskovic M.
267, 327
Bosnic T.
235, 314, 315
Bozinovska I.
209
Brackovic M.
314
Brboric J.
179, 180
Brezovska K.
23, 147, 149, 151, 205, 207
Brzilova K.
94, 211
Bigovic D.
313
Bilalagic N.
225
Bilensoy E.
2
Butikoski F.
211
Buturovic M.
187
C
C. Yu S.
35
Calis S.
45, 50
Caklovica S.
236
Carevic M.
261
Castle P.
3
Cerar A.
267
Cemerikic M.
126
Chakalarovski K.
259
Cornett C.
25
Couladis M.
284, 285
Crevar M.
196
Cruaud O.
43
Cudina O.
179, 180
Curcic M.
261
Curcic Jovanovic M.
262, 286, 326
Cukic N.
203
Cvejic J.
316
Cvetkovski A.
129
Cvetkovic N.
72
D
Dabovic Anastasovska J.
246
Damcevska D.
211
Dameska R.
67
Dastevska Mitevska M.
96
Davceva M.
41, 53
David F.
4
Debarlieva B.
211
Decristoforo C.
104
Della Loggia R.
11, 270
Dervisevic A.
243
Despotovska S.
156, 158, 160, 162, 166, 168
Dinic V.
69
Dimitrovska Manojlovic T. 122
Dimitrova M. J.
107
Dimitrovska A.
25, 175, 197, 199, 201, 203, 
205, 207, 226, 228, 244, 
Dimitrovska J.
233
Dimova D.
69
INDEKS NA AVTORI / 
AUTHOR INDEX
:

342
^ETVRTI KONGRES NA FARMACIJATA NA MAKEDONIJA SO ME\UNARODNO U^ESTVO
FOURTH CONGRESS OF PHARMACY OF MACEDONIA WITH INTERNATIONAL PARTICIPATION
Dimovski A. J.
14, 156, 158, 160, 162, 166, 168
Dizdarevic A.
187
Dizdarevic E.
188
Djarlieva M.
109
Djedjibegovic J.
225, 324, 338, 339
Djekic M.
286
Djono S.
324
Djordjevic A.
267, 268
Djordjev D.
244
Djordjevic K.
140
Djordjevic S.
320
Djordjevic-Milic V.
267, 268
Djukic A.
326
Djukic M.
262
Djukic-Cosic D.
286, 326
Djuric I.
286
Dobojak M.
238
Dolikoska C.
209
Doslov-Kokorus Z.
308
Dragojevic-Simic V.
268
Dragosevic G.
189
Dubrova-Koceva V.
211
Duchene D.
35
Dzambic M.
71
Dzikova S.
162, 166
E
Efremova D.
112
Efremova S.
112, 113
Elezovic A.
287
Elvedina K.
143
Evgenievska G.
213
Evstatieva Lj.
336
F
Fako S.
71
Fernandez C.
12, 100
Filipovic I.
312
Filkova S.
118
Fjalland B.
8
Frola E.
102
Frydenvang K.
29, 30
G
Gadzovska S.
283
Gasperlin M.
36
Gammelgaard B.
22
Gavrilovic J.
308
Georgiev V.
162, 166
Geskovski N.
45, 53, 59
Getov I. N.
107
Gjelstrup Kristensen H.
32
Gjorgjevic B.
323
Gjorgoski I.
290, 292, 294
Gjoseva S.
203
Glavas Dodov M.
24, 57
Gocevac D.
317
Gogusev J.
101
Gojkovic Bukarica Lj.
282
Goracinova K.
24, 38, 41, 43, 45, 47, 50, 51, 
53, 55, 57, 59, 96, 118,
Gosevska R.
265
Gorgevic S.
73
Govedarica B.
267, 268, 327
Grimstrup Madsen K.
22
Grozdanova A.
12, 147, 149, 151
Gruev T.
259, 276
Grujic N.
327
Guric Z.
72, 84
H
Hansen L. S.
27, 28
Hadzidedic S.
63, 83, 182
Hadzidedic S.
64, 71, 76, 78, 79, 80, 81, 82, 
188, 189
Hadzihasanovic M.
238
Hadzi-Petrushev N.
153
Hadzovic E.
64
Hiljadnikova Bajro M.
156, 158, 160, 168
Hincal A. A.
2, 45, 50, 
Hincal F.
250
Homsek I.
72, 73
Hristova O.
219
Hrncic N.
181
I
I. Petrova G.
130
Ibric S.
84
Ilash J.
5
Ilic M.
326
Ilijev V.
196
Ilievska M.
49, 231
Ilioska-Nabrezanec T.
90
Ilioska Zlatanovic S.
231
Injac R.
267, 268, 327
Isovic A.
236
Ivkovic B.
196
J
J. Alma
243
Jadranin M.
317
Jager A.
20, 304, 306
Jagodic D.
76
Jakimova D.
263, 321
Jandric A.
143
Janeva S.
211

343
^ETVRTI KONGRES NA FARMACIJATA NA MAKEDONIJA SO ME\UNARODNO U^ESTVO
FOURTH CONGRESS OF PHARMACY OF MACEDONIA WITH INTERNATIONAL PARTICIPATION
Janevic E. I.
43
Janevic-Ivanovska E.
39
Jankovic I.
179
Jerg-Simanovic D.
235, 315
Johansen N.T.
29, 30, 102
Josefsen K.
20, 304, 306
Josifovski T.
14, 156, 158, 160, 168
Jordanoska S.
57
Jovanovic A.
213
Jovanovic D.
140
Jovanovic M.
189, 262
Jovanovic T.
186
Jovanovska V.
231
Jurukovska S.
120