Methodology article

Authors’ contributions
TJ: study design and supervision, writing of manuscript, critical revision; JG: 
study design, acquisition of data (performing methods LN, EC, ND, spec-
trophotometric and fluorometric analysis, genome sequencing), writing of 
manuscript; ZB: acquisition of data (performing methods K1, K2, CsCl gradient 
ultracentrifugation, electrophoresis, PFGE, FIGE), drafting of manuscript; KZ: 
acquisition of data (performing methods LN, EC, ND), Illumina library prepara-
tion, genome sequencing; IŻ: Prototheca wickerhamii strain isolation and 
culture; RG: study design, analysis and interpretation of data, drafting of manu-
script, critical revision. All authors read and approved the final manuscript.
Author details
1
Department of Applied Microbiology, Institute of Microbiology, Faculty 
of Biology, University of Warsaw, I. Miecznikowa 1, 02-096 Warsaw, Poland. 
2
DNA Sequencing and Oligonucleotides Synthesis Laboratory at the Institute 
of Biochemistry and Biophysics, Polish Academy of Sciences, A. Pawińskiego 
5a, 02-106 Warsaw, Poland. 
3
Department of Clinical Microbiology, Children’s 
University Hospital of Cracow, Kraków, Poland. 
Acknowledgements
Not applicable.
Competing interests
The authors declare that they have no competing interests.
Availability of data and materials
All data generated or analyzed during this study are included in this published 
article.
Consent for publication
Not applicable.
Ethics approval and consent to participate
Not applicable.
Funding
The study was financed by the National Science Centre grants «PRELUDIUM» 
(2013/09/N/NZ2/00248) and «SONATA» (2014/15/D/NZ7/01797).
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Received: 21 March 2017 Accepted: 19 September 2017
Content courtesy of Springer Nature, terms of use apply. Rights reserved.

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Jagielski et al. Plant Methods (2017) 13:77