Получение товаров из кожи животных для медицинского применения и определение химического состава getting goods from animal skin for medical use and determination of chemical composition ibrohimjon Rahmonovich Asqarov

Тebl 4.
Qualitative and quantitative analysis of amino acids in collagen (100g)

Amino acid	In literature [17. P. 40]	In the study	Difference
Histidine	2,60	2,63	+0,03
Asparagine	0,42	0,44	+0,02
Aspartate	4,45	4,52	+0,07
Threonine	4,90	4,90	-
Serine	1,87	1,87	-
Glutamine	3,87	3,88	+0,01
Proline	7,19	7,23	+0,04
Glycine	11,82	11,97	+0,15
Valine	33,50	35,12	+1,62
Methionine	10,93	11,00	+0,07
Isoleucine	2,02	1,87	-0,15
Leucine	0,61	0,56	-0,05
Tyrosine	1,36	1,36	-
Phenylalanine	2,66	2,74	+0,08
Hydroxyproline	0,52	0,51	-0,01
Hydroxylysine	1,31	1,34	+0.03
Valine	9,21	10,69	+1,48
Methionine	0,76	0,74	-0,02

From the data presented in Table 4, it can be seen that the amino acid composition and the amount of both collagen proteins are almost the same. There are partial differences in some individual amino acids, which are mainly related to the separation process. This does not greatly affect its properties. It was also found that the most common amino acids in the composition of collagen practically did not differ in the amounts of glycine, proline and hydroxyproline.

Experimental part. Extraction of the aqueous mass of collagen. The first known hydrolysis method was used to obtain an aqueous mass of collagen by alkaline-salt hydrolysis of cattle skin [11. P. 14]. The second time the hydrolysis was carried out as follows. First washed in distilled water until neutralized, sorted; Hydrolysis in a 5% sodium hydroxide solution in a ratio of 1:10 All processes were carried out at a temperature of +21 - +230C.
Determination of the viscosity of the water mass of collagen. To determine the viscosity of collagen in 6% acetic acid 0.01; 0.02; 0.025; 0.03; 0.0375; Prepared 0.05% solutions. The dependence of the viscosity of collagen solutions on the concentration of solutions was determined on a Ubellode viscometer [ƞ]. The definition of intrinsic viscosity is calculated using the following formula:

Initially, the relative viscosity is found; (1)

From here is the relative viscosity; (2)

Intrinsic viscosity was found by the following formula. (3)

Here:
t_n – is the solution flow time
t₀ – is the solvent flow time
Cn – is the concentration of the solution
Amino acid analysis. The amino acid composition of collagen was determined by the Cohen method using HPLC, in the form of PTC derivatives of amino acids [18. S. 1-16].
Conclusion. The most optimal conditions for the isolation of the aqueous mass of collagen from the skin of cattle were found by the method of alkaline-salt hydrolysis without additives, the natural structure of which is preserved. After buffer extraction, a second hydrolysis was performed with 5% NaOH and 80 g/L Na2SO4 for 80 min. The results are validated by specific gravity, solubility and viscosity methods. It was also found that the most common amino acids in the composition of collagen practically did not differ in the amounts of glycine, proline and hydroxyproline.


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AUTHOR BIOGRAPHIES
Ibragim R. Askarov, Andijan State University
Doctor of Chemical Sciences, Professor of the Department of Chemistry, Honored Inventor of the Republic of Uzbekistan, Chairman of the "TABOBAT" Academy of Uzbekistan
Nurillo T. Muydinov, Andijan State University
PhD, Senior Lecturer of the Department of Chemistry