Quality control methods for

Buffered sodium chloride-peptone solution pH 7.0 
Procedure. Dissolve 3.56 g of potassium dihydrogen phosphate R, 7.23 g of 
disodium hydrogen phosphate R, 4.30 g of sodium chloride R and 1.0 g of dried 
peptone R (meat and casein) in sufficient water to produce 1000ml. Polysorbate 
20 R or polysorbate 80 R may be added, 0.001-0.01 g per ml. Sterilize in an 
autoclave at 121°C for 15 minutes. 
Casein-soybean digest agar 
Procedure. Dissolve 15.0g of pancreatic digest of casein R, 3.0g of papaic digest of 
soybean meal R, 5.0 g of sodium chloride R and 15.0 g of agar R in sufficient 
water to produce 1000 ml. Using sodium hydroxide (0.05 mol/l) VS adjust the 
pH to 7.1-7.5. Sterilize in an autoclave at 121°C for 15 minutes. 
Cetrimide agar 
Procedure. Dissolve 20.0 g of pancreatic digest of gelatin R, 1.4g of magnesium 
chloride R, 10.0 g of potassium sulfate R, 0.3 g of cetrimide R, 13.6 g of agar R 
and 10.0 ml of glycerol R insufficient water to produce 1000 ml. Heat to boiling 
for 1 minute with shaking. Using sodium hydroxide (0.05 mol/l) VS adjust the 
pH to 7.0-7.4. Sterilize in an autoclave at 121°C for 15 minutes. 
Deoxycholate citrate agar 
Procedure. Dissolve 10.0 g of beef extract R, 10.0 g of dried peptone R (meat), 10.0 
g of lactose R, 20.0 g of sodium citrate R, 1.0 g of iron(III) citrate R, 5.0 g of 
sodium deoxycholate, 13.5 g of agar R and 20 mg of neutral red R in sufficient 

---

Quality control methods for medicinal plant materials 
water to produce 1000 ml. Heat gently to boiling for 1 minute, cool to 50°C and 
adjust the pH to 7.1-7.5 using sodium hydroxide (0.05 mol/l) VS. Pour into Petri 
dishes. Do not heat in an autoclave. 
Enterobacteriaceae enrichment broth-Mossel 
Procedure. Dissolve 10.0g of pancreatic digest of gelatin R, 5.0g of glucose 
hydrate R, 20.0g of dehydrated ox bile R, 2.0g of potassium dihydrogen 
phosphate R, 8.0g of disodium hydrogen phosphate R and 15mg of brilliant 
green R in sufficient water to produce 1000ml. Heat to boiling for 30 minutes 
and cool immediately. Using sodium hydroxide (0.05 mol/l) VS adjust the pH to 
7.0-7.4. 
Lactose broth 
Procedure. Dissolve 3.0 g of beef extract R, 5.0 g of pancreatic digest of gelatin R 
and 5.0 g of lactose R in sufficient water to produce 1000 ml. Using sodium 
hydroxide (0.05 mol/l) VS adjust the pH to 6.7-7.1. Sterilize in an autoclave at 
121°C for 15 minutes. 
MacConkey agar 
Procedure. Dissolve 17.0g of pancreatic digest of gelatin R, 3.0g of dried peptone 
R (meat and casein), 10.0g of lactose R, 5.0g of sodium chloride R, 1.5g of bile 
salts R, 13.5g of agar R, 30mg of neutral red R and 1.0mg of crystal violet R in 
sufficient water to produce 1000ml. Using sodium hydroxide (0.05mol/l) VS 
adjust the pH to 6.9-7.3. Heat to boiling for 1 minute with constant shaking then 
sterilize in an autoclave at 121°C for 15 minutes.