Quality control methods for

Violet-red bile agar with glucose and lactose 
Procedure. Dissolve 3.0 g of water-soluble yeast extract R, 7.0 g of pancreatic 
digest of gelatin R, 1.5 g of bile salts R, 10.0 g of lactose R, 5.0 g of sodium 
chloride R, 10.0g of glucose hydrate R, 15.0 g of agar R, 30 mg of neutral red R 
and 2.0 mg of crystal violet R in sufficient water to produce 1000 ml. Heat to 
boiling and adjust the pH to 7.2-7.6 using sodium hydroxide (0.05 mol/l) VS. Do 
not heat in an autoclave. 
Xylose, lysine, deoxycholate agar 
Procedure. Dissolve 3.5 g of xylose R, 5.0 g of L-lysine R, 7.5 g of lactose R, 7.5 g of 
sucrose R, 5.0 g of sodium chloride R, 3.0 g of water-soluble yeast extract R, 0.08 
g of phenol red R, 13.5 g of agar R, 2.5 g of sodium deoxycholate R, 6.8 g of 
sodium thiosulfate R and 0.8g of brown ammonium iron(III) citrate R in 
sufficient water to produce 1000 ml. Using sodium hydroxide (0.05 mol/l) VS, 
adjust the pH to 7.2-7.6. Heat just to boiling, cool to 50°C and pour into Petri 
dishes. Do not heat in an autoclave. 
Strains of microorganisms 
The strains of microorganism referred to throughout the text are suitable, but 
others may be used if they have similar properties. The designations of the 
strains and the addresses from which they may be obtained are as follows: 
ATCC 
American Type Culture Collection, 12301 Park Lawn Drive, 
Rockville, MD 20852, USA. 
CIP 
Collection de l'Institut Pasteur, Service de la Collection Nationale 
de Cultures de Microorganismes (CNCM), 25 rue du Docteur 
Roux, F 75015 Paris, France. 
NCIMB 
National Collection of Industrial and Marine Bacteria, 23 St 
Machar Drive, Aberdeen AB24 3RY, Scotland. 

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Quality control methods for medicinal plant materials 
NCPF 
National Collection of Pathogenic Fungi, PHLS Mycology 
Reference Laboratory, Public Health Laboratory, Kingsdown, 
Bristol BS2 8EL, England. 
NCTC
National Collection of Type Cultures, Central Public Health 
Laboratory, 61 Colindale Avenue, London NW9 5HT, England. 

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Quality control methods for medicinal plant materials