Сборник V международной

Xulosa: Tibbiyotda ishlatiluvch turli xil massaj kremlarini tayyorlashda, Dorivor vositalar ishlab 
chiqarishda, Sochni mustahkamlash uchun shampunlarga muloyimlik berishda, ishlab chiqarishda xom- 
ashyoga efir moylaridan foydalanib ishlab chiqarish taklif etiladi.
SECONDARY METABOLITES FROM AERIAL PART OF SILENE TOMENTELLA
Yusupova U.Yu., Ramazonov N.Sh.
Institute o f the Chemistry o f Plant Substances 
[e-mail]
In the world research work carries out to find alternative source active compound from plants with a 
deep understanding biological impact and to extend exist source o f effective, eco-friendly and economical 
beneficial medicine. In pharmacy, to produce medicine that show complex biochemical effect on human 
body, medical plant is widely used. In recent years, in the field o f chemistry and pharmacology o f plant 
steroids, most of the work is devoted specifically to phytoecdysteroids, as the most original and promising 
group of compounds with metabolic activity. Herbs had been used by all cultures through history. There is 
a growing interest in the use of D-pinitol as a food supplement because o f its reported efficacy in lowering 
blood glucose levels with no side effects and nil toxicity.
Ecdysteroids are one o f the most widespread steroid compounds in nature. They were found in more 
than 90% of world’s animal species, named after arthropods, the species number o f which reaches 1 mil­
lion, as well as in some other invertebrates. The presence o f ecdysteroids were later discovered in many 
types of plants, including flowering ones.
A literature analysis showed that the family Caryophyllaceae comprised the greatest number o f spe­
cies containing ecdysteroids. Several ecdysteroids were isolated from plants o f the genus Silene, e.g., S. 
viridiflora, S. otites, and S. guntensis. Therefore, the endemic plant Silene popovii was studied in a search 
for new raw-material sources containing ecdysteroids. Caryophyllaceae revealed the largest number o f ec- 
dysteroid-containing species belonging to the Silene genera. In the present work the possible hypoglycemic 
activities of the sum of phytoecdysteroids from S. tomentella were analyzed. Besides, the chemical com­
pound of ethyl acetate extract was studied. The dried and ground aerial part o f S. tomentella (1.0 kg) was 
extracted four times with methanol (MeOH) (4 x 6 L) at room temperature. The extract was concentrated 
and diluted with an equal volume o f H2O. The resulting precipitate was filtered off. The MeOH was evap­
orated. The aqueous residue was extracted sequentially with CHCl3 and n-BuOH then with ethyl acetate. 
In our previous paper we worked with ethyl acetate fraction which was chromatographed over silica gel 
with elution by CHCl3-M eOH isolated eight phytoecdysteroids. In the present work we also isolated three 
compounds such as: a-ecdysone (1), D-pinitol (2) and 1,2,3,20-tetraacetate integristerone A (3) (Fig.).
’O H
H
2
CH
OH
O H
H
Isolated compounds from Silene tomentella.
These compounds were isolated from Silene tomentella for the first time.
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