elucidate the mechanismus of regulation o f vascular tone, which ensures the maintenance o f a normal level
o f blood pressure.
Objective: Study o f the action o f triazol-D-286 (4-(6-phenyl-7H-[1,2,4]-triazolo-[3,4-b]-[1,3,4]-
thiadiazin-3-yl)- aniline) on the contractile activity of rat aortic smooth muscle cells (SMCs). It has been
shown that the synthetic compound triazole-D-286 has a relaxing effect.
Materials and methods: In this regard, we carried out studies on the effect o f triazol-D-286 on the
contractile activity of SMC of the rat aorta in order to establish the dependence o f the relaxant activity of
triazol-D-286 on its structure . The studies were carried out on the isolated preparations o f the rat aorta, the
contractile activity of the aorta was assessed in the isometric mode using a tension sensor (FT.03, Grass,
USA) and a camera base. The experimental chamber was perfused with oxygenated carbogen (95% O2, 5%
CO2) the Krebs solution at a constant temperature of 37°C.
Results and discussion: The relaxant effect of this triazole was dose-dependent, and with an increase
in its concentration, the suppression degree of the contractions of aortic preparations induced by hyperpo
tassium solutions increased markedly. To further clarify the mechanism of action o f triazole, experiments
were performed on the dependence o f the relaxant effect o f D-286 on the concentration (5-50 pM ) o f Ca2+
ions in the incubation medium. It is known that in solutions containing no Ca2+ ions, hyperkalium solutions
do not cause contractions of aortic preparations, and the cumulative addition o f Ca2+ ions under these condi
tions is accompanied by the development o f contractions that reach the control amplitude at 2.5 mM CaCl2.
In particular, in the presence of 50 pM D-286, the addition o f 2.5 mM CaCl2 to the calcium-free solution
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