Investigating physiological and biochemical
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Muhammad Abdul Qayyum UAF 2015 Soil Env Sciences
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- S-907 5.80±0.13 5.59±0.24 5.42±0.25 1.78±0.16 2.50±0.29 2.57±0.26 C-99-3-115
Genotypes POD (Unit mg -1 protein) APX (Unit mg -1 protein) Control NaCl (100 mM) NaCl (200 mM) Control NaCl (100 mM) NaCl (200 mM) S-907 5.80±0.13 5.59±0.24 5.42±0.25 1.78±0.16 2.50±0.29 2.57±0.26 C-99-3-115 5.84±0.15 5.62±0.12 5.49±0.28 3.12±0.34 3.96±0.36 4.10±0.37 637-72 6.21±0.15 6.75±0.19 17.11±0.18 5.22±0.17 8.61±0.22 10.54±0.18 NO-303 6.24±0.33 6.84±0.11 7.16±0.20 4.31±0.25 6.67±0.32 8.14±0.17 Each value is an average of 3 replications ± SE 117 Similarly, APX was higher in salt tolerant genotypes (8.14-10.54 unit mg -1 protein) as compared to salt sensitive genotypes (2.57-4.10) at the salt level of 200 mM NaCl. On the basis of percent of control, POD activity in genotypes was in the order as NO-303 > 637-72 > C-99-3-115 > S-907 while for APX activity this order was as 637-72 > NO-303 > C-99-3-115 > S-907. 3.3.3.10 Lipid peroxidation (MDA) contents Significant increase in lipid peroxidation (MDA) was noted in linseed genotypes by the application of salt stress. A wide range of difference was noticed between salt sensitive and salt tolerant genotypes regarding their MDA contents and lipi d peroxidation levels. In salt sensitive genotypes S-907 and C-99-3-115, lipid peroxidation and MDA contents greatly increased with increasing salt concentration when compared with salt tolerant genotypes 637-72 and NO-303. At control, higher MDA contents were found in C-99-3-115 (5.45 nmol g -1 FW) and S-907 (5.11 nmol g -1 FW) while lowest MDA contents were found in NO-303 (4.58 nmol g -1 FW) followed by 637-72 (4.82 nmol g -1 FW). Salt sensitive genotypes showed progressive increase in their MDA concentration and it was 8.50 nmol g -1 FW in S-907 genotype and 8.40 nmol g -1 FW in C-99-3-115 genotype at 100 mM NaCl. On the other hand, salt tolerant genotypes had lower MDA contents (6.37-6.99 nmol g -1 FW) at 100 mM NaCl concentration. Similarly, at 200 mM NaCl, MDA concentrations of NO-303 (8.30 nmol g -1 FW) and 637-72 (8.39 nmol g -1 FW) genotypes were the minimum while that of C-99-3-115 (11.23 nmol g -1 FW) and S-907 (11.70 nmol g -1 FW) was the maximum (Table 3.3.10). Linseed genotypes possessed similar order for MDA contents for both salinity levels and it was S-907 > C-99-3-115 > NO-303 > 637-72 at 200 mM NaCl. 118 Table 3.3.10. Lipid peroxidation (MDA) contents of linseed as affected by salt stress Download 1.66 Mb. Do'stlaringiz bilan baham: |
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