Investigating physiological and biochemical

3.4.2.4 Measurements of oil contents 
To obtain oil contents by solvent extraction, method of Popa et al. (2012) was 
used. The crushed seeds of linseed were extracted with petroleum ether (Merck, 
40-60°C) in a Soxhlet apparatus and the remaining solvent was removed by 
distillation. After extraction, the oil samples were filtered and stored. Extracted oil 
samples had a light yellow color.
3.4.2.5 Statistical analysis 
All the data presented in this study are mean of three replicates. Analysis of 
variance (ANOVA) was performed by using a statistical package, Statistix 8.1. 
Significant differences among treatments were considered at the P ≤ 0.05. 
3.4.3. Results 
3.4.3.1 Effect of salt stress on number of branches in linseed 
 
Number of branches plant
-1
of all the genotypes were significantly affected by the 
application of salinity and number of branches were significantly reduced between 
control and 100 mM NaCl (Table 3.4.1). The maximum number of branches plant
-1
(9.00) were recorded at control while the minimum number of branches plant
-1
(2.67) 
were observed at 200 mM NaCl. There was no significant difference among linseed 

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130 
genotypes regarding number of branches plant
-1
at control but salt stress adversely 
affected the genotypes in the production of branches. Genotypes showed sharp 
decrease in number of branches plant
-1
with increasing levels of salinity. The number 
of branches of salt tolerant genotypes were more in salt tolerant genotypes when 
compared with salt sensitive genotypes and it varied with salt treatment from 8.67 and 
7.67 in 637-72 and NO-303 genotypes respectively. Similarly, salt sensitive 
genotypes S-907 and C-99-3-115 possessed 6.33 and 6.00 number of branches plant
-1
at 100 mM NaCl (Table 3.4.1). Linseed genotypes did not show greater differences in 
number of branches but effect of salinity was more pronounced. At 200 mM NaCl, the 
maximum number of branches were recorded in NO-303 (3.33) followed by 637-72 
and S-907 (3.00) while the minimum number of branches were noted in C-99-3-115 
(2.67).