Сборник V международной

STUDY OF THE TEMPERATURE RESISTANCE OF «SALMONELLA 
POLYVALENT BACTERIOPHAGE» - “MEDIPHAG”
Jumaniyazova M.B.
Institute o f Microbiology o f the Academy o f Sciences o f the Republic o f Uzbekistan
[e-mail], tel. [phone]
Relevance of the topic. Bacteriophage titer stability is an important marker in the treatment o f dis­
ease in medicine. The conditions that allow phage stability to be maintained vary greatly depending on the 
particular phage.
Aim. Evaluation of the thermal stability o f «Salmonella polyvalent bacteriophage» - “MediPhag” 
(Series No. 010520; Release date 05.2020; Valid until 04.2022) produced by LLC “AZIA IMMUNO 
PREPARAT”
Materials and methods. In the study of the temperature resistance of the bacteriophage, 5 ml of 
phage filtrate was added to the test tubes and kept in a water bath at 30° C to 60° C for a separate half-hour. 
Bacteriophage stored in the refrigerator at 5-10 ° C was used as a control option. The Gracia method was 
used to compare the titers of the phages in all the solutions.
Salmonella m oscow  №14
Salmonella enteritidis N27
Salm onella paratyphi В
Salm onella newport №33
ха р о р а т узгариш и (30 -60 )
ха р о р а т у зга р иш и (30 -60 )
харорат узгариши (30 -60 )
харорат узгариши (30 -60 )
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The results obtained. The temperature resistance of the lytic activity o f Salmonella polyvalent 
bacteriophage was studied separately with Salmonella enteritidis, Salmonella moscow, Salmonella java, 
Salmonella paratyphi B, Salmonella typhimurium, Salmonella newport, Salmonella agama strains. during 
the study it was found that the average amount o f lytic activity varied from 7.2 * 109 ± 0.3 * 109 to 4.8 * 
109 ± 0.8 * 109.
Conclusions. We found that the stability o f Salmonella polyvalent bacteriophage gradually decreas­
es with increasing temperature. This feature of bacteriophages is important in transporting them to the 
desired location. This therapeutic bacteriophage is registered in Uzbekistan under the number R№ DV / M 
02782/10/19.