Sterilization methods in microbiology Sterilization objects

Chemical control

• 5th grade - integrating indicators. These indicators respond to all critical parameters of the sterilization method. The performance of this class of indicators is compared with inactivation highly resistant microorganisms.

• 6th grade - emulator indicators. These indicators must respond to all control values of the critical parameters of the sterilization method.
• (biological method)

coccoid
rod-shaped
crimped
Morphology of bacteria,
pathogens of human diseases

Staphylococcus

Streptococci

Sarcins

Rod-shaped bacteria

Coryneform bacteria Corinebacterium sp.

Clostridium tetani

Clostridium tetani
Clostridium difficile
Bacillus anthracis

Vibrio bacteria

Twisted bacteria. Spirilla and spirochetes

The main morphological forms of opportunistic fungi, pathogens of human diseases

Pathogen name	Cell shape	Reproduction method
Candida spp.	Yeast cell	Budding
Aspergillus spp.	Septated mycelium
Penicillium spp .	Septated mycelium
Mucor spp .	Nonseptate mycelium
Rhizopus spp.	Nonseptate mycelium

Candida albicans budding cells

The main morphological forms of protozoa, pathogens of human diseases

Pathogen name	Vegetative form cells
Trichomonas vaginalis
Entamoeba histolytica
Balantidium coli
Giardia lamblia
Toxoplasma gondii
Trypanosoma sp.
Leishmania sp.
Plasmodium sp.