“Oziq-ovqat va funksional oziq-ovqat mahsulotlarini ishlab chiqarishda mahalliy xom ashyo resurslaridan foydalanish
muammolari” ilmiy-amaliy anjumani Shahrisabz-2022 yil 22-23 aprel
175
Xloroplast matK yopiq urug’lilarning eng o'zgaruvchan barkod genlaridan biri bo'lib, quruqlik o'simliklari
uchun "barkod" bo'lishi taklif qilingan. O’simlik xloroplast barkod genlarida matK muhim universal nomzod
genlardan hisoblanib, u taxminan 1570 juft nukleotidlardan iboratdir[3].
matK barkod geni amplifikatsiyasi uchun K.Vijayan va C. Tsou [1] tomonidan matK X 5′-
TAATTTACGATCAATTCATTC-3′, matK 5 5′- GTTCTAGCACAAGAAAGTCG-3′ va 390F 5′-
CGATCTATTCATTCAATATTTC-3′,
1326R
5′-TCT
AGCACACGAAAGTCGAAGT-3′
praymerlari tavsiya etilgan.
Olib borilgan tadqiqotlar natijasida Atriplex pratovii o’simligini rbcL barkod geni amplifikatsiyasi
uchun rbcLa-F va rbcLa-R praymeri yordamida 600 juft nukleotiddan, rbcLa-F va rbcLajf634-R
praymeri yordamida esa 650 juft nukleotiddan iborat taxminiy uzunlikdagi PZR mahsuloti borligini
aniqlandi.
Atriplex pratovii o’simligining matK geni matK-1RKIM-F va matK-3FKIM-R praymerlari
yordamida 900 juft nukleotiddan iborat taxminiy uzunlikdagi PZR maxsuloti olindi.
Adabiyotlar.
1. K. Vijayan and C. H. Tsou “DNA barcoding in plants: taxonomy in a new Perspective”
//Current Science december 2010// 1530-1541 p
2. J.Heckenhauer and J.Barfuss “Uneversal Multiplexable matK primers for DNA barcoding of
Angiosperms”//Applications in Plant Sciencess 2016\\ 1-7 p
3. W.Dong, Chao Xu, Changhao Li, Jiahui Sun, Yunjuan Zuo, Shuo Shi, Tao Cheng, Junjie Guo
and Shiliang Zhou “ycf1, the most promising plastid DNA barcode of land plants” //Scientific Reports
2015//1-4 p
4. R. Rajphriyadharshini, J. Weerasena “DNA Barcoding of Medicinal plant: a Systemic Review”
//International Journal of Pharmaceutical Science Invention June 2020 ‖ PP. 6-16 p
5. Jing YU, Jian-HUE, Shi-Liang ZHOU “New universal matK Primers for DNA barcoding
angiosperms” //Journal of Systematics and Evolution 2011//176-181 p
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