Ўзбекистон республикaси олий тaълим, фан ва инновациялар вaзирлиги

Таблица 1. 
Состав жирных кислот (в виде метиловых эфиров)
образцов
,
 ГХ, % от массы кислот 
Жирная кислота 
Содержание 
Миристиновая , 14:0 
0,10 
Пентадекановая, 15:0 
0,09 
Пальмитиновая, 16:0 
12,42 
Пальмитолеиновая, 16:1 
0,12 
Маргариновая, 17:0 
0,10 
Стеариновая, 18:0 
3,85 
Олеиновая, 18:1ω9 
Линоленовая, 18:3 ω3* 
31,06 
Линолевая, 18:2ω6 
51,40 
Арахиновая, 20:0 
0,27 
Эйкозеновая, 20:1 ω9 
0,27 
Бегеновая, 22:0 
0,22 
Лигноцериновая, 24:0 
0,10 
∑
насыщенных ЖК 
17,15 
∑
ненасыщенных ЖК 
82,85 
*Эта пара жирных кислот в использованных условиях ГХ не разделяется и 
выходит одним пиком 
Исходя из вышесказанного, продукты на основе сои содержат много 
незаменимых аминокислот, витаминов, микро- и макроэлементов, которые 
полезны для здоровья человека. 
 
METHODS OF DIAGNOSING MYELOID LEUKEMIYA DISEASE 
Kadirova Z.A., Riskiyev B., Xojiyeva D.K., Saidova M.T., 
Mingnorov Sh.N., Maxamatxo'jayev Х. 
National University of Uzbekistan, Institute of Hematology and Blood 
Transfusion Research. 
[e-mail]
  
In recent years, the prevalence of oncological diseases has been 
increasing. The main reasons for this are changes in climatic conditions, 
environmental pollution, depletion of the ozone layer, exposure to radioactive 
substances and rays, the use of chemical materials and pesticides in agriculture. 

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The problem of oncological diseases is the most central in terms of complexity, 
importance and danger for mankind. Millions of people die from this disease 
every year. 
Chronic myeloid leukemia (CML) is a complex disease that occurs 
worldwide in both developed and underdeveloped countries. The exact causes 
of the disease are still unknown to medicine, but the disease can be caused by 
viral, endogenous, chemical factors and radioactive rays, intoxication, chemical 
factors, genetic disorders, hormonal imbalance, and many cases of viral 
diseases. This disease can occur in young children, women, men and the elderly. 
In the past, effective treatment methods for leukemia were not yet studied, but 
now it is on the list of treatable diseases, and there are treatments that can 
overcome the disease. 
Symptoms of myeloid leukemia may not be noticeable at first. Mainly, 
there are changes in the composition of the blood, in which normo-, erito- and 
megaloblasts increase. In the peripheral blood, anemia, a decrease in the amount 
of platelets, changes in the leukogram, a sudden stop of blood formation, etc. 
are observed. 
Microscopic diagnosis of blood composition in myeloid leukemia usually 
uses a general blood test. Through this analysis, the quantity and quality 
indicators of blood-forming elements are studied. Hemoglobin testing is one of 
the most important and basic laboratory tests. Pathological changes in the 
number of erythrocytes and their morphology are considered an important 
indicator, allowing for diagnostic and prognostic determination of the disease. 
Also, in the blood analysis, the increase in the number of leukocytes and the 
decrease in the number of neutrophils, changes in lymphocytes, thrombocytes 
are also important. 
Today, modern methods of detecting myeloid leukemia have been 
effectively implemented in Uzbekistan, and various scientific and research 
works are being carried out for more perfect study of this disease, timely 
detection and proper treatment. In this case, after preliminary blood tests, 
isolation and quantification of mRNA from the bcr-abl (variant M-bcr) chimeric 
gene and the abl gene using the "AmpliSens" kit of reagents is performed. To 
perform PCR, it is important to first isolate the bcr-abl gene from mRNA in 
clinical material. For this, mRNA is extracted from peripheral blood cells or 
bone marrow (according to the Khomchinsky method). Then the reverse 
transcription reaction is carried out, the amplification process is carried out with 
a mixture of two oligonucleotides. Control of amplification products, i.e. 
detection, is carried out in "real time" mode, the result of bcr-abl cDNA 
amplification is recorded through the fluorescent channel. The main stages of 

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the endogenous internal control assay are RNA isolation, transport, storage, 
isolation, RNA reverse transcription reaction and direct cDNA amplification, 
control, which allows accurate calculation of the amount of mRNA in the bcr-
abl chimeric gene.