Inaktivatsiyaga uchrashi
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Bog'liq6.6 Laboratoriya
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- Uskunalar
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Sinov materiali: tupurik (2 marta suyultirilgan so’lak)
Reaktivlar: 1% kraxmal eritmasi, Lyugol reagenti, distillangan suv, Trommer reaksiyasi uchun kerak bo’lgan reaktivlar: 5%li o’yuvchi natriy, 5%-li mis sulfat (tajriba probirkalariga avval o’yuvchi natriy qo’shiladi, so’ngra tomchilatib mis sulfatdan solinadi va past olovda qaynaguncha qizdiriladi). Uskunalar: pipetkalar, probirkalar, termostat, alkogol lampasi, muzli hammom Ishning bajarilish tartibi: 2 ta probirkaga 1 ml dan suyultirilgan so’lak solinadi. Ulardan bittasi past olovda 2-3 minut davomida qaynatib olinadi. Shundan so’ng har ikkalasiga 1 ml dan kraxmal eritmasidan solib, 10 minut 38°S li termostatga qo’yiladi. Keyin har ikkala probirkadagi suyuqlik ikkiga bo’linib, Trommer va Lyugol reaksiyalari o’tkaziladi. Shuni aytish kerakki, qaynatilgan so’lakli probirkada kraxmalning fermentativ gidrolizi bo’lmaydi. A.Mashg’ulot davomida olingan xulosalar: _________________________________________________________________________ _________________________________________________________________________ _________________________________________________________________________ _________________________________________________________________________ _________________________________________________________________________ B.Laboratoriya ishi bo’yicha nazorat savollari: 1. Ferment tushunchasiga to’liq ta’rif qanday? ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ 2. Fermentlar qanday tabiatli moddalar va necha guruhga bo`linadi? ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ 3. Tempratura ta’sir ettirilgan amilaza fermentiga Trommer va Lyugol reaksiyalarini o’tkazishdagi natijani farqi qanaqa?Ingibitorlar qanday xususiyatiga ega? ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ 4. Aktivatorlar qanday xususiyatiga ega? _________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ Talabaning ism familiyasi _____________________________________________________ Guruhi ___________________Sana: _____________________________ Download 158.36 Kb. Do'stlaringiz bilan baham: 
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