Technology of storage and primary processing of agricultural products

Double fertilization
The process of double fertilization in indoor seed plants was first described by the Russian scientist S.G.Navashin (1898). When the dust tube that enters the ovarian sac collides with the ovarian apparatus, it ruptures and the fluid inside is poured into the ovary. In this case, one sperm fuses with the egg cell nucleus to form a zygote, while the other sperm fertilizes the central nucleus. This process is called double fertilization. Father, an egg cell (zygote) is then Murtaza, the father of the central core of the seed endosperm develops. Fertilized egg cell (zygote) 2p diploid endosperm
Figure 2
the nucleus has a 3p triploid chromosome. in a cotton plant, the addition of the sperm nucleus to the central nucleus occurs 18-23 hours after pollination, and the fertilization of the egg occurs 2-3 hours later. The first division of the triploid endosperm nucleus begins one hour after fertilization , and the development of the zygote by division 3 days later.
Get the job done.
Dust growing on the mother column of the cotton flower
method of counting the number of tubes
The method of counting the number of pollen tubes growing inside the maternal column was developed by I.D. Romanov , which consists of: Collecting the opened cotton flower 24-48 hours after pollination , the maternal column is separated and stored (fixed) in a container with 96 ° alcohol . To prepare the drug, take one column and make several transverse thin incisions using a manual anatomical lesion. 2-3 drops of "Iodine Phenol" paint are dropped on a series of cutters on the subject window . Using a closed-loop microscope on top, you can determine the number of dust tubes growing into the mother’s beak.
The growing tissue in the dust the top of the pipes round apertures in the viewing part of the formation, so this gap is called "vacuum tubes". Dust tube traces appear brown in cross section because they have their own plasma.
6- LABORATORY WORK.