Vazirligi urganch davlat universiteti tabiiy fanlar fakulteti " biologiya" kafedrasi "tasdiqlandi"

 
 

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Amaliy mashg‟ulot-2 
Blast dasturi yordamida nukleotid ketma-ketliklarini solishtirish 
BLAST (Basic Local Alignment Search Tool – asosiy lokal tekislanishni 
izlash vositasi) bu kompyuter dasturlaridan biri bo‟lib, u oqsil yoki nuklein 
kislotalar gomologlarini mavjud bazadan izlab topishga xizmat qiladi. Sodda qilib 
tushuntirilganda sizda oqsil yoki nuklein kislotalarning ma‟lum bir ketma-ketligi 
bor. Lekin siz bu nuklein kislota qaysi turga tegishli ekanligini bilmaysiz. BLAST 
sizga mana shu qo‟lingizdagi nukleotidlar ketma-ketligi qaysi turga tegishli 
ekanligini aniqlashga yordam beradi. Albatta u bazada mavjud bo‟lgan millionlab 
namunalarga solishtirish asosida sizga kerakli natijani chiqarib beradi. BLAST 
dasturini 1990-yilda AQSHlik bir guruh tadqiqotchilar ishlab chiqqanlar.
1990-
yildan buyon BLAST NCBI saytida hamma uchun mavjud.
BLAST dasturi online 
rejimda ishlaydigan dasturlardan hisoblanadi. Ya‟ni bu dasturni ishlatish uchun 
avvalo internetga ulangan bo‟lishi kerak. 
1- topshiriq 
1.Quyidagi nukleotid ketma-ketligini taxlil qiling. Ushu ketma-ketlikda 
qaysi nukleotidlar ortiqcha. Ushbu organism turini aniqlang 
ggctggaatagtggggactgccttaagcctccttattcgggccgaactaagccaacccgggtcacttttagga
gatgaccaaatttataatgttattgttactgcccacgcctcgtaataattttctttatagtaatgccaattcttattggagggttc
ggaaactgactcgtcccactgatgattggagcccctgatatggcattcccacgaataaacaatataagcttctgattatta
cctccgtcattcctgttattattagcctcttctggtgttgaggctggcgctggaactgggtgaacagtatatccgccactcg
caggtaatctgcccacgcaggcgcatcagtagacttaacaatcttttcacttcatctggcgggagtatcatcaatcttggg
tgcagtcaactttatcaccacaattattaacatgaaacccccagctatctcccagtaccaaacgccgctatttgtgtgagc
cgtactagtaacagctgtccttctctattgtcactaccagttttagctgccggaattacaatacttcttacagaccgtaatctt
aatactaattcttcgacccggcaggagg 
2. Quyidagi nukleotid ketma-ketligini taxlil qiling. Blast dasturi yordamida 
turni aniqlang 
gctaacttagatgaagggatcaaagcatttttttccagcccccatctttctatgtcagtcctcaagacttgccttttgtggag
aatggaggagtggcagtgttgactagcagaaaagtcttgcacatggacgtccgagggaataaagtaagattggacaa 
tggttcagaaattacttatgataaatgtttaattgcaacaggcggtactccacgtaaccttccagcacttgaaagagcagg
agaagaggtgcaaaagcaaacaactctattcagaaagatagaagatttt aaatcattggagaagatttcaagaaatgtt 
cagtctattgttattattggtggtggattcctgggtagcgaattggcctgtgctttggga 
agacgtgcac 
ggaacacaga 
actcgaggtgattcagatatttccagagaaaggaaacatgggcaagattctaccagaatacctcagcaattggacaact
gagaaagtaaggaaagagggtgttaatgtcatgcccaatacagttgtgaaatctgcaaaatatcaagataagaaagtta
ttctgaaacttaaggatgggcaaacaataaagactgatcatg 
3. Quyidagi nukleotid ketma-ketligini taxlil qiling. Blast dasturi yordamida 
turni aniqlang 
cggccgcagcgatgggcagtgagcctgtggggaagccgacggggcctaaggagcgcctcggggagac
ggcagcggggacccggcctcaattctgagtgcggagggggcgcgcgagcgcggcgcaccaagcgagttgctggc
cagttcttgaggggcgagcggcccgtgtggtccgcatcccgatcccggggctccgtgacccggccctgagcatctcg
tgggggtcaaggagcgtcttccgaggccgcccggggcccagcgctgccgccaccgagccccggggcgccccctg
tatggccgagctgccccctcgccggccgcccgggcgccgcagcggctgaggtcgctgggatcgcccggccgccg
cccccatcgccggcccctgcatgcccggcgcccgggtcgcagcccacctggacgctctgggccccctggtctccta
cgtgcagccaccgctgctcccctctatgttctacgtgggcctgttcttcgtcaatgtgctgatcctctactacgccttcctc

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atggaatacatcgttctcaatgtgggcctcgtctttctgcccgaggacttggaccaggcgctggtggaccttggtgtact
ctcagaccctggctctggcctttacgatgcagactcagagctcgacgtcttcgatggatatttggaataaggtcttgatta
ctgttccccacttgccttccgccatccacaactcttgacctggaccagccgcctgtatcatgccgctgccactggctggg
ggcaccatctggacagggatgggaccccaggaagaggcccgcctgctcgtgccaacaaccaggttcctgccccaa
cccaggcgtctgccaggagaagtgaggactcagtgccgagatggggagttgccagatcctccgaacgcctcgcctg
ccacccagctctccgctgctctggacctggcttgtgacttgccacctttgtgatggatgtttacaggaacccaaccagac
gtcacccacagccgcacctgcctcccccactcaacctccgggaaggacacggagagctgaggtggcaaacggact
catgcttaggcttttcccgtcagcattgtttgatgctggttgaccttaagcctgggaagacttcagacccaagactgttgat
catagtttcagggtgtcactctggagggctatgccagggcccaggacagggtttaacttctccaggttgacagaactga
gatactgcactggggaaggccatgctgtgtctggctagaaatctatttttggaagtgtgagtggtgtgtaaagtgtgaatg
gtgtagatccttgaatttccataaacctgtaaaagcaatacctagaggctgacttatttcattaaaaaaaaaatgtaagcaa 
4. Quyidagi nukleotid ketma-ketligini taxlil qiling. Blast dasturi yordamida 
turni aniqlang 
Caaccaaaacaagcattccattcgtatgcaaaccaaaacgccaagtacttaattactatctttaaaacaaaaaa
acccataaaaattgcgcacaaacatacaaatatgcgaccccaaaaatttaaccattaaaaacaaaaaatttaatatattat
agccctatgtacgtcgtgcattaactgctagtccccatgcatataagcatgtacatattattattaatattacatagtacatatt
attattgatcgtacatagcacatatcatgtcaaataactccagtcaacatgcgtatcaccaccattagatcacgagcttaat
taccatgccgcgtgaaaccagcaacccgcttggcagggatccctcttctcgctccgggcccataaatcgtgggggttt
ctattgatgaactttaacaggcatctggttcttacttcaggaccatctcatctaaaatcgcccactctttccccttaaataaga
catctcgatggactaatgactaatcagcccatgctcacacataactgaggtttcatacatttggtattttttaatttttgggga
tgcttggactcagccatggccgtcaaaggccctaacacagtcaaatcaattgtagctggacttcatggaactcatgatcc
ggcacgacaatc 
5.
Quyidagi nukleotid ketma-ketligini taxlil qiling. Blast dasturi yordamida 
turni aniqlang
Gaagtctatcctggaacgaatggacccgtctggccgcgaccaggtgtatctacgctgcgctccgtctgcagggttgct
gaagacgtcgtgcagcttggcatccttaactgtttccatcagggatctggacgtagcgtccaatctgctgccggccctg
ccg 
gcgagtgttgggtgagagtgcgtgttttttgtgtgtagctgctgcgggtgagtgagtgagwgagagagagagagaga
gagagagagagasaasgaaagaaagttggattttggaacatgtacatgttgagaagtaaagaaagcagatgtt 
tgtaagaaaa ctatttttag cattgaaaat cccctggaaa ggggagggtgcaccgttccc agagatactg 
caataccggg tcgatgcgtg gagtggacgg agcaagcccc 
tattccatct ccctgctcca aaaatcaatt taatatatgg tccccagata agggacgtatca 
6.
Quyidagi nukleotid ketma-ketligini taxlil qiling. Blast dasturi yordamida 
turni aniqlang 
gtggtttgttaaggtggcagagcctggtaa 
ttgcaaaaga 
tctaagccct 
ttaatccagaggttcaactc 
ctctccttaa 
ctaatgcttaactttatccttctctacattattaaccccttgacctttattatccctattctactagccacagccttcct 
aaccctagttgaacgaaaaatcctaggttatatgcaattccgcaaaggcccaaacgtggttggtccatat 
ggtctcctacaacccattgctgacggactc aaactattta ctaaagaacctgtacgaccctccttttcct 
cccaattcctgtttctagctacacctactactgctctaaccttagccctcctaatatgaatgcccctacctcttccacac 
tcaatcctaaacttaaacctaggcttattattcatcctagctatctcaagcttaacagtttacactattttagggtcagg 
ttgagcctcaaactccaaatacgccctcataggagctctccgtgcagtagcacaaaccatctcttacgaa 
gtaacactcgctctaatcctcctagccctaattatctttgtaggcggttttacactccatacatttaatttaagccaaga 
aaccatctga ctaatcatcc ccgcatgacc tcttgccatg atgtggtata 

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7.
Quyidagi nukleotid ketma-ketligini taxlil qiling. Blast dasturi yordamida 
turni aniqlang 
ctttatattcggcgcctgatccggactagtcggagcctgcctaagcatcttaatgcgaatagaactcactcagccagggt 
ccctatttgg cagcgatcag atctttaatg tcctagtaaccgcacacgcc ttcatcataa tcttctttat ggttataccg 
attataatcgggggctttggaaattgactaatcccactaataatcggcacaccagacatggccttcccccgtataaacaa
cataagcttc tgactcctcc cccccgctct tctccttctt ctatcctcct cttatgtcgaagcgggtgctggaacaggat 
gaactgttta tcccccactg tctggaaacc tagtacactcaggcccatcagtagacctagcaattttctccctccacctt 
gccggagcctcatcaattctcggagcaattaacttcatcaccacttgcattaacataaaacctaaatcaatacccatgttta
acttccct ttattcgttt gatctgtcat aatcactgcc atcatactac tcctagcactgcccgttctt gcagcagcaa 
tcactatatt attaacagac cgaaacctga acacctcctt 
8.
Quyidagi nukleotid ketma-ketligini taxlil qiling. Blast dasturi yordamida 
turni aniqlang
taaagacata attggaattg gtttggcagg taattcttag ttgacagtaa tgtgtctaggtaatgttaga ttaatggttt 
cagaacataaacttaaataatctctcgcataacatggatttggccacagtattggattatctcttaatttgactggtcacgag
aaataagcaatycttgttagataagatactaagttactagtttcaagcccatacagtgatggcgtacataactgatctattct
ggcctttggttgttttgtcaagcacataagattaataaagtccattcgttcctctttaaaaggcctctggttaatgtgttctata
cattaaatttataacctggcatttcatgtcttaggcacatatatttagctcttttttctctttctgttttcagacccacatacctgat
gtcaccgattaactgaaactggacctacattcagattgattgatcttgcaagatttgatatggtattatt